Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,596,773	(C)6→7	intergenic (‑4331/+1844)	hipB ← / ← lsrK	antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,596,773	1	.	C	100.0%	74.4 / NA	24	intergenic (‑4331/+1844)	hipB/lsrK	antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (17/7);  total (17/7)

TGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCGCTTGCTTTATCGCCCAAAAAAGTAATATTTTGCGATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCCC‑GTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGCCTTCACTCGAGACAT  >  NC_000913/1596550‑1597008                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            tGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCGCTTGCTTTATCGCCCAAAAAAGTAATATTTTGCGATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCCCCGTGGTGATGCTGACATTCCCGACGcc                                                                                                                                                                                                                   >  1:37385/1‑251 (MQ=255)            aTACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCGCTTGCTTTATCGCCCAAAAAAGTAATATTTTGCGATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCAt                                                                                                                                                                                                        >  2:64324/1‑251 (MQ=255)                cGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCGCTTGCTTTATCGCCCAAAAAAGTAATATTTTGCGATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAAcc                                                                                                                                                                                                    <  1:99306/251‑1 (MQ=255)                   tACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCGCTTGCTTTATCGCCCAAAAAAGTAATATTTTGCGATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACCTTCCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCg                                                                                                                                                                                                   >  2:17132/1‑249 (MQ=255)                       aaaaTTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCGCTTGCTTTATCGCCCAAAAAAGTAATATTTTGCGATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGa                                                                                                                                                                                              >  1:191/1‑250 (MQ=255)                               ttcgatactgtcccACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCGCTTGCTTTATCGCCCAAAAAAGTAATATTTTGCGATACGCACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCCCCGTGGTGATGCTGCCATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTtcctcc                                                                                                                                                                                     >  2:50238/12‑251 (MQ=255)                                                         gatgatGTCGCATCCATCAGGTTCAGTGTGCCGATACCGCTTGCTTTATCGCCCAAAAAAGTAATATTTTGCGATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTAc                                                                                                                                                           >  2:132766/1‑251 (MQ=255)                                                                                cAGTGTGCCGATACCGCTTGCTTTATCGCCCAAAAAAGTAATATTTTGCGATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGcc                                                                                                                                     <  1:202720/250‑1 (MQ=255)                                                                                     tgCCGATACCGCTTGCTTTATCGCCCAAAAAAGTAATATTTTGCGATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCAt                                                                                                                                >  2:141119/1‑250 (MQ=255)                                                                                        cGATACCGCTTGCTTTATCGCCCAAAAAAGTAATATTTTGCGATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAAcc                                                                                                                            >  1:357932/1‑251 (MQ=255)                                                                                                            cccAAAAAAGTAATATTTTGCGATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCCCCGTGGTGATGCTGACATTCCCGACTCCGGTTTCTCCATAACCGCGTGAACTTCCTCCGTTGCTGACAACAACCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACAAAGCTTAGTTCCGAGAttcg                                                                                                        >  2:309143/1‑249 (MQ=255)                                                                                                               aaaaaaGTAATATTTTGCGATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTGATTGACCTCCCACATTCCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTGCTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGGGTAGTGa                                                                                                     <  2:242035/251‑1 (MQ=255)                                                                                                                     gTAATATTTTGCGATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACgg                                                                                               <  2:320677/251‑1 (MQ=255)                                                                                                                              tGCGATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTa                                                                                       <  1:174513/250‑1 (MQ=255)                                                                                                                              tGCGATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTAt                                                                                      >  1:329725/1‑251 (MQ=255)                                                                                                                                       aaCTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAAc                                                                               >  2:120774/1‑249 (MQ=255)                                                                                                                                                 cATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTtgcc                                                                   >  2:107721/1‑251 (MQ=255)                                                                                                                                                                                aaCACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATa                                    <  1:201908/251‑1 (MQ=255)                                                                                                                                                                                    ccAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTc                                >  1:194702/1‑251 (MQ=255)                                                                                                                                                                                         ggtggtATAGACATTCTTATTGACCTCCCACATTCCCCCCCGTGGAGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATCACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGa                            >  1:60730/1‑250 (MQ=255)                                                                                                                                                                                           tggtATAGACATTCTTATTGACCTCCCACATTCCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATc                         >  1:257025/1‑251 (MQ=255)                                                                                                                                                                                                           aTTGACCTCCCACATTCCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGCCTTCACTc         >  1:159934/1‑251 (MQ=255)                                                                                                                                                                                                              gACCTCCCACATTCCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGCCTTCACTCgag      <  1:223146/251‑1 (MQ=255)                                                                                                                                                                                                                  tCCCACATTCCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCCTAACCGAGTGAACTTCCTCCGTTGCTGACAACACGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATACCCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGCCTTCACTCGAGACAt  >  1:241854/1‑251 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            TGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCGCTTGCTTTATCGCCCAAAAAAGTAATATTTTGCGATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCCC‑GTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGCCTTCACTCGAGACAT  >  NC_000913/1596550‑1597008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑