Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,584,256	Δ1 bp	coding (2506/3513 nt)	hsdR ←	type I restriction enzyme EcoKI endonuclease subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,584,256	0	T	.	100.0%	178.5 / NA	39	coding (2506/3513 nt)	hsdR	type I restriction enzyme EcoKI endonuclease subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (19/20);  total (19/20)

GCTGCGGTCACGGTTAAACGTGGCCAGACCGATGATACGCTGGAGCTTCGCCACCAGTTGTTCATGGCTGTGCTCGGCAAAACTGCGGCCATCCGCTTCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTTTATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGAATATCGACGCCGGTCGT‑‑CA‑GCAGGTCGACGGTTACCACGATATTGGGCAGCCGCTCTTTATTGAAGCGGGTGATCATGGTCTGCACTTTGCGCGCGTCTTTATCGGCATCACCGGTGATCTTGATGATCGCGTCGTGCTCCAGTTGCGG  >  NC_000913/4584011‑4584505                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                             gCTGCGGTCACGGTTAAACGTGGCCAGACCGATGATACGCTGGAGCTTCGCCACCAGTTGTTCATGGCTGTGCTCGGCAAAACTGCGGCCATCCGCTTCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTTGTTT‑ATTCAc                                                                                                                                                                                                                                                        <  1:298631/251‑1 (MQ=255)    gCGGTCACGGTTAAACGTGGCCAGACCGATGATACGCTGGAGCTTCGCCACCAGTTGTTCATGGCTGTGCTCGGCAAAACTGCGGCCATCCGCTTCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCAcc                                                                                                                                                                                                                                                       <  1:265425/249‑1 (MQ=255)            ggTTAAACGTGGCCAGACCGATGATACGCTGGAGCTTCGCCACCAGTTGTTCATGGCTGTGCTCCGCAAAACTGCGGCCATCCGCTTCGGCGATTTTCTAGGTTTCTGCATAGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCCGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAACGCTGGTTT‑ATTCACCTCCGGGCAt                                                                                                                                                                                                                                              >  1:184488/1‑250 (MQ=255)                                     aCGCTGGAGCTTCGCCACCAGTTGTTCATGGCTGTGCTCGGCAAAACTGCGGCCATCCGCTTCGGTGATTTTATAGGTTTCTGAAGCGGTAATTTCATTGACCAGCGGTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCt                                                                                                                                                                                                                     <  1:275247/250‑1 (MQ=255)                                      cGCTGGAGCTTCGCCACCAGTTGTTCATGGCTGTGCTCGGCAAAACTGCGGCCATCCGCTTCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGt                                                                                                                                                                                                                   <  2:238110/251‑1 (MQ=255)                                          ggAGCTTCGCCACCAGTTGTTCATGGCTGTGCTCGGCAAAACTGCGGCCATCCGCTTCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTc                                                                                                                                                                                                                 <  1:314947/249‑1 (MQ=255)                                                       caGTTGTTCATGGCTGTGCTCGGCAATACTGCGGCCATCCGCTTCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCTGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTACGTGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCAGCt                                                                                                                                                                                                  <  2:262779/251‑1 (MQ=255)                                                                aTGGCTGTGCTCGGCAAAACTGCGGCCATCCGCTTCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACttt                                                                                                                                                                                         <  1:279701/251‑1 (MQ=255)                                                                aTGGCGGTGCTCGGCAAAACTGCGGCCATCCGCTTCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACttt                                                                                                                                                                                         <  2:104568/251‑1 (MQ=255)                                                                  ggctggtcTCGGCAAAACTGCGGCCATCCGCTTCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTGCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCTGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTa                                                                                                                                                                                        <  2:214570/243‑1 (MQ=255)                                                                           cGGCAAAACTGCGGCCATCCGCTTCGGTGATTTTAGAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCTTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATGCACCTCCGGGCATAAGCTCGTTGCGCGACCGGTCATCTGTTCTTACAGAATTCGGCTGCGTACTTTACGCAGGAaca                                                                                                                                                                              <  1:154670/251‑1 (MQ=255)                                                                              cAAAACTGCGGCCATCCGCTTCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACg                                                                                                                                                                            >  2:193234/1‑250 (MQ=255)                                                                                aaaCTGCGGCCATCCGCTTCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGat                                                                                                                                                                          >  2:300702/1‑250 (MQ=255)                                                                                       ggCCATCCGCTTCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTAc                                                                                                                                                                     >  1:181288/1‑248 (MQ=255)                                                                                          cataggctccGGTGATTTTATCGGTTTCTGAATCGGTAATTTCCTTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGCCGCTCTCCAGCGTGCTGTCGATTTCGACACAGTCAGAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCTCGGACTTTCATCTGTTCGTACAGACTGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGAt                                                                                                                                                                 >  2:258402/10‑249 (MQ=255)                                                                                           aTCCGCTTCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCg                                                                                                                                                               >  2:48431/1‑250 (MQ=255)                                                                                             ccGCTTCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACg                                                                                                                                                            <  1:183679/251‑1 (MQ=255)                                                                                                  tCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGaa                                                                                                                                                         <  1:48431/249‑1 (MQ=255)                                                                                                            tataGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGACTGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGAATATCGACGCCgg                                                                                                                                             >  2:88845/1‑251 (MQ=255)                                                                                                                       gAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGAATATCGACGCCGGTCGT‑‑ca‑gc                                                                                                                                  <  2:8256/248‑1 (MQ=255)                                                                                                                             gTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCCGACCAATAGTGTCGACGCTCTCCAGCGCGCTGTAGATATCGACACAGACAACAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCATGGCGCGGCCTTTCATCTGATCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGAATATCGACGCCGGTCGT‑‑CA‑GCAGGTCGACg                                                                                                                         >  1:41496/1‑251 (MQ=255)                                                                                                                              tAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGAATATCGACGCCGGTCGT‑‑CA‑GCAGGTCGACgg                                                                                                                        >  1:265478/1‑251 (MQ=255)                                                                                                                                  ttCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGAATATCGACGCCGGTCGT‑‑CA‑GCAGGTCGACGGTTAc                                                                                                                    >  2:20890/1‑251 (MQ=255)                                                                                                                                           cAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGAATATCGACGCCGGTCGT‑‑CA‑GCAGGTCGACGGTTACCACGATAtt                                                                                                           <  1:300702/251‑1 (MQ=255)                                                                                                                                            aGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGTCCTTTCATCTGCTCGTACAGAATGCGGTTGCGTACTTTACGCAGGAACACGATATTACAGATCGCTGGAATATCGACGTGGGTCGT‑‑CA‑GCAGTCCGACGGTTCCCACGATATTg                                                                                                          >  2:292199/1‑251 (MQ=255)                                                                                                                                                     aGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGAATATCGACGCCGGTCGT‑‑CA‑GCAGGTCGACGGTTACCACGATATTGGGCAGCCGc                                                                                                 >  2:75625/1‑251 (MQ=255)                                                                                                                                                             cTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGAATATCGACGCCGGTCGT‑‑CA‑GCAGGTCGACGGTTACCACGATATTGGGCAGCCGCTCTTTAtt                                                                                         >  2:278630/1‑251 (MQ=255)                                                                                                                                                                cGGGCGCACCACCACCGGACGCAGGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTG‑ATTCACCTCCGGGCATAAGCGCGTTGCGTGGCCTTTCATCTGTTGGTTCTGAATGCTTCTGCGTACTTTACTCAGTTACTCGATATTACAGATCGGCTGAATATGTACGCCGGTCGT‑‑CA‑GTAGTCCGACGGTTACCACGATATTGGGCAGCCGCTCTTTATTGaa                                                                                      <  1:372182/251‑1 (MQ=255)                                                                                                                                                                  ggCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGAATATCGACGCCGGTCGT‑‑CA‑GCAGGTCGACGGTTACCACGATATTGGGCAGCCGCTCTTTATTGAAGc                                                                                    >  1:322757/1‑251 (MQ=255)                                                                                                                                                                                               aCGCTCTCCAGCGTGCTGTAGATATCGACACCGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTCCGCAGGAACACGATATTACAGATCGACGGAATATCGACGCCGGTCGT‑‑CA‑GCAGGTCGACGGTTACCACGATATTGGGCAGCCGCTCTTTATTGAAGCGGGGGATCATGGTCTGCACTTTGcgcgcg                                                       >  1:176809/1‑251 (MQ=255)                                                                                                                                                                                                    ctcCAGCGTGCGGTAGATATCTACACAGTCAAAAATCTTATAGCTGGTTT‑ATTCACCTCCGGGCAGAAGCGCGTTGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGAATATCGACGCCGGTCGT‑‑CA‑GCAGGTCGACGTGTACCACGATATTGGGCAGCCGCTCTTTATTGAAGCGGGTGATCATGGTCTGCACGATGCGCGCGTCt                                                    <  2:184488/249‑1 (MQ=255)                                                                                                                                                                                                                     tatCGACTCAGGCAAAGTTCTTAAAGCTGGTGT‑ATTCCCCTCCGGGCATAAGCTCATTGCGCTGGCTTGCATCTGTTCGGACAGAATGCGGCTGCTTACTGTACGCAGTACCTCGATATTACAGTTCGACGGAATATTGCTGTTTGTTTTTTCAAGCAGAACG‑CGGC‑AT‑ACGATATTGGGCAGCCGCTCTTTATTGAAGCGGGGGATCATGGTCTGCACTTTGCGCGCGTCTTTATCGGCATCACCGGTg                                  <  1:360802/250‑1 (MQ=255)                                                                                                                                                                                                                       tCGACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGAATATCGACGCCGGTCGT‑‑CA‑GCAGGTCGACGGTTACCACGATATTGGGCAGCCGCTCTTTATTGAAGCGGGAGATCATGGTCGGCACTTTGCGAGCGTCTTTATCGGCATCACCGGTGATc                               >  2:62686/1‑251 (MQ=255)                                                                                                                                                                                                                         gACACAGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTCCAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGAATATCGACGCCGGTCGT‑‑CA‑GCAGGTCGACGGTTACCACGATATTGGGCAGCCGCTCTTTATTGAAGCGGGTGATCATGGTCTGCACTTTGCGCGCGTCTTTATCGGCATCACCGGTGATCtt                             >  1:69702/1‑251 (MQ=255)                                                                                                                                                                                                                            acaGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGaa                                                                                                                                                         <  1:26812/127‑1 (MQ=255)                                                                                                                                                                                                                            acaGTCAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGaa                                                                                                                                                         >  2:26812/1‑127 (MQ=255)                                                                                                                                                                                                                                 cAAAAATCTTAAAGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGAATATCGACGCCGGTCGT‑‑CA‑GCAGGTCGACGGTTACCACGATATTGGGCAGCCGCTCTTTATTGAAGCGGGTGATCATGGTCTGCACTTTGCGCGCGTCTTTATCGGCATCACCGGTGATCTTGATGATcg                     >  1:249531/1‑251 (MQ=255)                                                                                                                                                                                                                                           aaaGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGAATATCGACGCCGGTCGT‑‑CA‑GCAGGTCGACGGTTACCACGATATTGGGCAGCCGCTCTTTATTGAAGCGGGTGATCATGGTCTGCACTTTGCGCGCGTCTTTATCGGCATCACCGGTGATCTTGATGATCGCGTCGTGCTc           <  2:137952/251‑1 (MQ=255)                                                                                                                                                                                                                                             aGCTGGTTT‑ATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGAATATCGACGCCGGTCGT‑‑CA‑GCAGGTCGACGGTTACCACGATATTGGGCAGCCGCTCTTTATTGAAGCGGGTGATCATGGTCTGCACTTTGCGCGCGTCTTTATCGGCATCACCGGTGATCTTGATGATCGCGTCGTGCTCCa         <  1:87973/251‑1 (MQ=255)                                                                                                                                                                                                                                                  ggtttATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGAATATCGACGCCGGTCGT‑‑CA‑GCAGGTCGACGGTTACCACGATATTGGGCAGCCGCTCTTTATTGAAGCGGGTGATCATGGTCTGCACTTTGCGCGCGTCTTTATCGGCATCACCGGTGATCTTGATGATCGCGTCGTGCTCCAGt       <  1:108784/247‑1 (MQ=255)                                                                                                                                                                                                                                                     ttATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGAATATCGACGCCGGTCGT‑‑CA‑GCAGGTCGACGGTTACCACGATATTGGGCAGCCGCTCTTTATTGAAGCGGGTGATCATGGTCTGCACTTTGCGCGCGTCTTTATCGGCATCACCGGTGATCTTGATGATCGCGTCGTGCTCCAGTTGCgg  >  1:153152/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                             GCTGCGGTCACGGTTAAACGTGGCCAGACCGATGATACGCTGGAGCTTCGCCACCAGTTGTTCATGGCTGTGCTCGGCAAAACTGCGGCCATCCGCTTCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTTTATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGATATTACAGATCGACGGAATATCGACGCCGGTCGT‑‑CA‑GCAGGTCGACGGTTACCACGATATTGGGCAGCCGCTCTTTATTGAAGCGGGTGATCATGGTCTGCACTTTGCGCGCGTCTTTATCGGCATCACCGGTGATCTTGATGATCGCGTCGTGCTCCAGTTGCGG  >  NC_000913/4584011‑4584505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑