Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,942,635	(T)7→6	intergenic (‑68/‑61)	gcvA ← / → gcvB	DNA‑binding transcriptional dual regulator GcvA/small regulatory RNA GcvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,942,635	0	T	.	100.0%	85.1 / NA	19	intergenic (‑68/‑61)	gcvA/gcvB	DNA‑binding transcriptional dual regulator GcvA/small regulatory RNA GcvB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (9/10);  total (9/10)

GGAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCC  >  NC_000913/2942408‑2942881                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       ggAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTT‑AATCTGAGCCATTATAAATTGTcc                                                                                                                                                                                                                                <  1:220323/251‑1 (MQ=255)                       aTCCGCAAGAGACTTGATTTGATGACTTACTGCTGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTT‑AATCTGAGCCATTATAAAGTGTCCGTTGAGCTTCTACCAGCAAATa                                                                                                                                                                                                          <  2:246187/251‑1 (MQ=255)                                         ttgatGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTT‑AATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACt                                                                                                                                                                                         <  2:21905/250‑1 (MQ=255)                                               aCTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTT‑AATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTg                                                                                                                                                                                    >  1:290221/1‑249 (MQ=255)                                                                 cacaAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAACTGGCGTGCTGCGGCATCAAAAACTCGTCAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTT‑AATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTTAGCCGGCACCAAAAGttttt                                                                                                                                                                >  2:110935/1‑250 (MQ=255)                                                                             ttctGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATGTAGCGGTGGTAAGCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACGATGTAACCTATTAGTTTTTT‑AATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGa                                                                                                                                                                             <  1:288276/226‑1 (MQ=255)                                                                             ttctGCTCTGCGAGTGAAACTTAAATGGCGTGCTGCGGCTTCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACCTGGCTATTATATTTTAAAGTTAACTGAATTTAACACTTAGATCACACTATGTAACCTATTAGTTTTTT‑AATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGa                                                                                                                                                                             >  2:288276/1‑226 (MQ=255)                                                                              tctGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTT‑AATCTGAGCCATta                                                                                                                                                                                                                                          <  1:157991/164‑1 (MQ=255)                                                                              tctGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTT‑AATCTGAGCCATta                                                                                                                                                                                                                                          >  2:157991/1‑164 (MQ=255)                                                                                                 ttAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTT‑AATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCtt                                                                                                                                >  2:227956/1‑251 (MQ=255)                                                                                                                     tCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTT‑AATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGGTGTGAtgttgtgttg                                                                                                            >  2:12713/1‑251 (MQ=255)                                                                                                                      cAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTT‑AATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGAtgttgtgttg                                                                                                            <  2:91421/250‑1 (MQ=255)                                                                                                                                                                             ttGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTT‑AATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCtttttt                                                      <  1:18191/249‑1 (MQ=255)                                                                                                                                                                                               aaCAATTAGATCACACTATGTAACCTATTAGTTTTTT‑AATCTGAGCCATTATAAAtt                                                                                                                                                                                                                                    <  2:226737/57‑1 (MQ=255)                                                                                                                                                                                               aaCAATTAGATCACACTATGTAACCTATTAGTTTTTT‑AATCTGAGCCATTATAAAtt                                                                                                                                                                                                                                    >  1:226737/1‑57 (MQ=255)                                                                                                                                                                                                     tAGATCACACTATGTAACCTATTAGTTTTTT‑AATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCctgtctg                            >  1:216886/1‑251 (MQ=255)                                                                                                                                                                                                        aTCACACTATGTAACCTATTAGTTTTTT‑AATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTcc                         <  1:256064/251‑1 (MQ=255)                                                                                                                                                                                                                aTGTAACCTATTAGTTTTTT‑AATCTGAGCGATTATAAATTGTCCGTTGCGCTTCTACCAGCAAATAACTATAGTGGCGACACTTCCTGAGCCGGAACGGAACGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTAAATTTACCCTGTCTGTCCATAGTGAt                 >  2:114088/1‑251 (MQ=255)                                                                                                                                                                                                                  gTAACCTATTAGTTTTTT‑AATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTa               <  2:216886/251‑1 (MQ=255)                                                                                                                                                                                                                              gttttttAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCg    <  1:283478/250‑1 (MQ=255)                                                                                                                                                                                                                                tttttAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGTTTAATGTAGCACCGcc  >  2:80271/1‑251 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       GGAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCC  >  NC_000913/2942408‑2942881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑