Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,602,017	(C)7→6	coding (200/2019 nt)	hyfB →	hydrogenase 4 component B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,602,017	0	C	.	100.0%	110.3 / NA	24	coding (200/2019 nt)	hyfB	hydrogenase 4 component B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (13/11);  total (13/11)

GGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCT  >  NC_000913/2601777‑2602258                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  ggCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC‑TTTTGAAttt                                                                                                                                                                                                                                         <  1:93326/250‑1 (MQ=255)   cGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGTCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC‑TTTTGAATTTGcc                                                                                                                                                                                                                                      <  1:370755/251‑1 (MQ=255)              cccTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTACTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC‑TTTTGAATTTGCCGATTTAACCCt                                                                                                                                                                                                                           <  2:84413/251‑1 (MQ=255)                             aGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC‑TTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCct                                                                                                                                                                                                            >  2:302542/1‑251 (MQ=255)                               gAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC‑TTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCctct                                                                                                                                                                                                          <  1:374540/251‑1 (MQ=255)                                                     gCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC‑TTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGtt                                                                                                                                                                                      <  1:365395/249‑1 (MQ=255)                                                           attaACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC‑TTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTAGCTCCtt                                                                                                                                                                              <  2:205379/251‑1 (MQ=255)                                                              aaCCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC‑TTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCt                                                                                                                                                                           >  1:90982/1‑251 (MQ=255)                                                                     tCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC‑TTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtggtg                                                                                                                                                                    >  2:49893/1‑251 (MQ=255)                                                                      cGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC‑TTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtggtgg                                                                                                                                                                   >  2:321051/1‑251 (MQ=255)                                                                         tGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC‑TTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTACTTGCTGGTGGTGGttt                                                                                                                                                                >  1:21183/1‑251 (MQ=255)                                                                                ctATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTCCTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATAATCAGCGGACTTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC‑TTTTGAATTTGCCGATTTAACCCTGCGAATGGCTAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctct                                                                                                                                                         >  1:349772/1‑251 (MQ=255)                                                                                      gTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTAGTGTAACTTTAGTCGCCCGTTTTGCCCCCC‑TTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTAt                                                                                                                                                     <  2:252149/249‑1 (MQ=255)                                                                                       tttGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCCGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGCTTTGCCTCCC‑TTTTGAATTTGCCGATTTAACTCTTCGAATGGATCGCCTCTCGGCTTTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTct                                                                                                                                                  >  2:282527/1‑250 (MQ=255)                                                                                                                 aCTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAAATACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC‑TTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAggg                                                                                                                        >  1:294704/1‑251 (MQ=255)                                                                                                                                      tATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC‑TTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATggg                                                                                                   >  1:324515/1‑251 (MQ=255)                                                                                                                                                           aTCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC‑TTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTAGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTcat                                                                              >  1:182588/1‑251 (MQ=255)                                                                                                                                                                 ggtggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC‑TTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTcatcgcatc                                                                        <  2:103406/251‑1 (MQ=255)                                                                                                                                                                 ggtggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC‑TTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTcatcgcatc                                                                        <  2:324515/251‑1 (MQ=255)                                                                                                                                                                                             ggAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC‑TTTTGAATTTGCCGATTTAACCCTGCGAATGTATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGTTGATGGACAAc                                            <  1:83384/251‑1 (MQ=255)                                                                                                                                                                                                               gCTGGTGTAACTTTAGTCGCCCCTTTTGCCCCCC‑TTTTGAATTTGCCGCTTTAACCCTGCGAATGGATCGCCTCTCGGCATTTATGGGGCTGGTTATCTCCTTGCTGGCGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGCATACGAGGCGAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCCTCGATGGTTGCCCTGCTGGTGATGGACAACGACTTTTGGTTCATCGTg                          >  2:38882/1‑251 (MQ=255)                                                                                                                                                                                                                       aaCTTTAGTCGCCCGTTTTGCCCCCC‑TTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTg                   <  1:288476/250‑1 (MQ=255)                                                                                                                                                                                                                                 gCCCGTTTTGCCCCCC‑TTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCATCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCg        >  1:153494/1‑251 (MQ=255)                                                                                                                                                                                                                                 gCCCGTTTTGCCCCCC‑TTTTGAATTTGCCGATTTAACCCTGAGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGCCTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCg        >  2:318168/1‑251 (MQ=255)                                                                                                                                                                                                                                        tttgccccccTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCt  >  1:223165/5‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  GGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCT  >  NC_000913/2601777‑2602258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑