Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,326,966 (C)6→5 coding (858/1185 nt) atoB → acetyl‑CoA acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,326,9660C.96.4% 116.9 / ‑3.8 28coding (858/1185 nt)atoBacetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base . (15/12);  total (15/13)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAGAAAACCTTCGTCTTCAGTCAAGACGAATTCCCGAAAGCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAAAACCTGGGCTTTGATTCTGAGAAAGTGAATGTCAACGGCGGGGCCATCGCGCTCGGGCATCCTATCGGTGCCAGTGGTGCTCGTATTCTGGTCACACTATTACATGCCATGCAGGCACG  >  NC_000913/2326732‑2327214
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                        
aaagaaaACCTTCGTCTTCAGTCAAGACGAATTCCCGAAAGCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATggg                                                                                                                                                                                                                                         <  1:196844/251‑1 (MQ=255)
               cTTCAGTCAAGACGAATTCCCGAAAGCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCAc                                                                                                                                                                                                                          >  2:279493/1‑251 (MQ=255)
                       aaGACGAATTCCCGAAAGCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCaaaa                                                                                                                                                                                                                    >  1:58733/1‑249 (MQ=255)
                               atCCCGATAGCGAATTCAACGGCTGAAGAATTAGGTGCATAGCTCCAGGCGTGCGAAAAAGCGGGCACAGTCACCGCTGGGAACGCTTCGGGTATTAAGGTCGGTGCAGCCGCTCCGGTGATTATGGGAGAATCGGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTGGCATTAAAAGTTATGCCAGCGGGGAGGTGCCCCC‑GCATTGATGGGGATGGGGCGAGTACCTGCCACGCAAAGAGCGTTACaa                                                                                                                                                                                                          <  2:345506/250‑1 (MQ=255)
                                     aaaGCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCaaaa                                                                                                                                                                                                                    <  2:281842/235‑1 (MQ=255)
                                     aaaGCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCaaaa                                                                                                                                                                                                                    >  1:281842/1‑235 (MQ=255)
                                                    gCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCCACTGGCg                                                                                                                                                                                     >  2:279605/1‑251 (MQ=255)
                                                           cGTTAGGTGCATTGCGCCAGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGTGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACCTGCGGATATTg                                                                                                                                                                              >  1:142012/1‑251 (MQ=255)
                                                               aGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGGATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTgggggtgcttgattc                                                                                                                                                                          >  2:205699/1‑238 (MQ=255)
                                                                       tGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCATTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAg                                                                                                                                                                   <  1:4333/250‑1 (MQ=255)
                                                                            ccGGGCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTTGCGGATATTTGTCTCATTTAGGGTAAt                                                                                                                                                             >  2:116757/1‑251 (MQ=255)
                                                                                  ttCGGTAAAGCAGGACCAGTAACCGCTGGGAACGCGTCTGGTATTAACGACGGAGCTGCCGCTCTGGTGCTTATGGAAGAATCTGCGCCGCTGGCAGCAGACCTTCCCCCCCTGGCTCGCCTTAAAAGTTATGCCAGCGGTGGAGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCCACTGGCGGGTATTGATCTTATTGAGGCTattgtagc                                                                                                                                                        >  2:23316/1‑243 (MQ=255)
                                                                                             aGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGAGGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATAGTGATCTCATTGAGGCTAATGAAGCATTTGCGGcaca                                                                                                                                            <  2:19198/251‑1 (MQ=255)
                                                                                                aaCAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGtt                                                                                                                                         <  1:230831/251‑1 (MQ=255)
                                                                                                 aCAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTc                                                                                                                                        <  2:142012/251‑1 (MQ=255)
                                                                                                         cGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGt                                                                                                                                <  1:206496/251‑1 (MQ=255)
                                                                                                          gCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGtt                                                                                                                               >  1:141959/1‑251 (MQ=255)
                                                                                                           cTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGAGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGAGTGCCCCC‑GCATTGATGGGTATGCGGCCAGTACCTGCCACGCAAAAAGCGTTACACCTGGCGGGGGTGCAACAGGCGGCTATTGATATCGTTGGGGCTAATGAAGCAATTGCTGCACAAGTCCTTGCCggtg                                                                                                                              >  1:156718/1‑248 (MQ=255)
                                                                                                                  cgcgTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGaaaaa                                                                                                                       <  2:58733/251‑1 (MQ=255)
                                                                                                                     gTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCGGCAACTGGCGGGTATTGGAATCATTGAGGGCCATGAAGCAATTTCTGCGCCGTTCCttttccttggggaaaaac                                                                                                                     >  2:189873/1‑234 (MQ=255)
                                                                                                                      tCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTTGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCCACCGGCGGGTATTTATCTCAATTAGGCTCATAAAGCATTTTCCGCACCGTTCCTTGCCGTTGGGAAAAACCtt                                                                                                                   >  2:194836/1‑250 (MQ=255)
                                                                                                                             ttAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCCAAGTTCCTTGCCGTTGGGAAAAAACTGGGCTTTg                                                                                                            >  1:205201/1‑251 (MQ=255)
                                                                                                                                     ggTGCTGCCGATCTCGTGATTATGGAAGCATCTGCGGAGCTGGCAGCAGGCCTTACCCACCTCGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCCACTGGCGGGTATTTCTTTCATTGCGGGTAACTACGCATTTGCCGCACAGGTCCTTGCCGTTGGGGAAAACCCGGGCTTTTAttttg                                                                                                      >  2:166440/1‑246 (MQ=255)
                                                                                                                                                         tATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGGTATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAAAACCCGGGCTTTGATTCTGAGAAAGTGAATGTCAAcggcgg                                                                                >  2:123047/1‑251 (MQ=255)
                                                                                                                                                                      gcggcgCGTGCAGCAGGGCTAGCCCCCCTGGATCGCATTAAAAGTTATGCCATCTGGGGCGT‑TCCCTCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGGTACAACTGGCGGGGCTGCATCTGGCGGATATGGTTCTCATTGAGGCTAATGAAGCATTTGCTGCACCGTTGCTTTCCGTTGGGAAAAACCTGGGCTTGGATTCTGAGAAAGTGAATGTCAACGGCGGGGCGATcgcg                                                                      <  2:331897/248‑1 (MQ=255)
                                                                                                                                                                                            ccccccTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAAAACCTGGGCTTTGATTCTGAGAAAGTGAATGTCAACGGCGGGGCCATCGCGCTCGGGCATCCTATCGGTGCCAgtg                                             <  1:282332/251‑1 (MQ=255)
                                                                                                                                                                                               cccTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAAAACCTGGGCTTTGATTCTGAGAAAGTGAATGTCAACGGCGGGGCCATCGCGCTCGGGCa                                                              <  1:314467/231‑1 (MQ=255)
                                                                                                                                                                                                       cGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCC‑GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAAAACCTGG‑CTTTGATTCTGAGAAAGTGAATGTCAACGGCGGGGCCATCGCGCTCGGGCATCCTATCGTTGCCAGTGGTGCTCGTAtt                                  <  1:314626/250‑1 (MQ=255)
                                                                                                                                                                                                                                ggcttgcccccGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAAAACCTGGGCTTTGATTCTGAGAAAGTGAATGTCAACGGCGGGGCCATCGCGCTCGGGCATCCTATCGGTGCCAGTGGTGCTCGTATTCTGGTCACACTATTAcatgccatg          <  2:248403/245‑1 (MQ=255)
                                                                                                                                                                                                                                       ccccGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAAAACCTGGGCTTTGATTCTGAGAAAGTGAATGTCAACGGCGGGGCCATCGCGCTCGGGCATCCTATCGGTGCCAGTGGTGCTCGTATTCTGGTCACACTATTACATGCCATGCAGGCAc   <  2:141959/251‑1 (MQ=255)
                                                                                                                                                                                                                                       ccccGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAAAACCTGGGCTTTGATTCTGAGAAAGTGAATGTCAACGGCGGGGCCATCGCGCTCGGGCATCCTATCGGTGCCAGTGGTGCGCGTATTCTGGTCACACTATTACATGCCATGCAGGCAc   <  1:310176/251‑1 (MQ=255)
                                                                                                                                                                                                                                        cccGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAAAACCTGGGCTTTGATTCTGAGAAAGTGAATGTCAACGGCGGGGCCATCGCGCTCGGGCATCCTATCGGTGCCAGTGGTGCTCGTATTCTGGTCACACTATTACATGCCATGCAGGCAcg  <  2:299088/251‑1 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                        
AAAGAAAACCTTCGTCTTCAGTCAAGACGAATTCCCGAAAGCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAAAACCTGGGCTTTGATTCTGAGAAAGTGAATGTCAACGGCGGGGCCATCGCGCTCGGGCATCCTATCGGTGCCAGTGGTGCTCGTATTCTGGTCACACTATTACATGCCATGCAGGCACG  >  NC_000913/2326732‑2327214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑