Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	697,300	(T)6→5	intergenic (‑167/+213)	metT ← / ← asnB	tRNA‑Met/asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	697,300	0	T	.	100.0%	84.5 / NA	19	intergenic (‑167/+213)	metT/asnB	tRNA‑Met/asparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (9/10);  total (9/10)

GTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCG  >  NC_000913/697059‑697548                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                         gtggCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTT‑CCCACCCTAc                                                                                                                                                                                                                                                >  1:26618/1‑251 (MQ=255)     cTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACCGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTT‑CCCACCCTACAGcc                                                                                                                                                                                                                                            >  2:270690/1‑251 (MQ=255)                           gACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTT‑CCCACCCTACAGCCATTCTTTTGTCATACAGGATGa                                                                                                                                                                                                                      <  1:70936/251‑1 (MQ=255)                                                    tctAACCAACTGAGGTCCGAAGCAATCTTTTTTTTCGCGATACCTTATCGGCGTTACGGAGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAACGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTT‑CCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAgtggtg                                                                                                                                                                                             >  2:31748/1‑251 (MQ=255)                                                       aaCCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATTCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTT‑CCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTggg                                                                                                                                                                                           <  2:125837/250‑1 (MQ=255)                                                        aCCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTT‑CCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGaa                                                                                                                                                                                         <  1:112279/251‑1 (MQ=255)                                                                      gTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATGCGTCAATTATTGTTTACTTTGTGTTTTT‑CCCACCCTACAGCCAGTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGTTGGGAAATTAATCTATGaaa                                                                                                                                                                           <  2:225390/251‑1 (MQ=255)                                                                                                             ggggCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTT‑CCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTgg                                                                                                                                    >  2:12575/1‑251 (MQ=255)                                                                                                                      tATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTT‑CCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCgg                                                                                                                           >  2:74436/1‑251 (MQ=255)                                                                                                                             ataGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTT‑CCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAAGTAATCTAGGAAATACTGGGCTACAGTGATGAGTTGTCaaa                                                                                                                                               <  1:226989/224‑1 (MQ=255)                                                                                                                             ataGAGACTTGCAGAGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGCACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTT‑CCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCaaa                                                                                                                                               >  2:226989/1‑224 (MQ=255)                                                                                                                                                      ttttCAAGGAAAATTGCTCGAAAGTGACTTTTTGTTTAGTTTGCGAACAGCGTTGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTT‑CCCGCCCTACAGCCATTCTTTTGTCATACATGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGATGTCAAACAGTGATGTGGCAAACCCGGAACATGTCCTTACTGCATATCAGAATcaaca                                                                                            <  2:349785/250‑1 (MQ=255)                                                                                                                                                      ttttCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTT‑CCCACCCTACATCCATTCTTTTGCCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATcaaca                                                                                            <  2:349790/250‑1 (MQ=255)                                                                                                                                                        ttCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTT‑CCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTTGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAg                                                                                          <  1:310398/250‑1 (MQ=255)                                                                                                                                                                           aaGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTT‑CCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAAc                                                                        >  1:276591/1‑249 (MQ=255)                                                                                                                                                                             gTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTT‑CCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGcc                                                                    <  1:129504/251‑1 (MQ=255)                                                                                                                                                                                                  gAACAGCGTGCCGCTATATTCGTCAATTATTGTTTTCTTTGTGTTTTT‑CCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATTCTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTTAAACAGCCCCGGATTTCACCGGGGCTGtt                                               <  2:287718/251‑1 (MQ=255)                                                                                                                                                                                                          tGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTT‑CCAACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCAttt                                       >  1:334219/1‑251 (MQ=255)                                                                                                                                                                                                                      cGTCAATTATTGTTTACTTTGTGTTTTT‑CCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACg                           >  1:97648/1‑251 (MQ=255)                                                                                                                                                                                                                                           gtgtttttCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAAcc       <  2:276591/248‑1 (MQ=255)                                                                                                                                                                                                                                            tgtttttCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAAcc                                                                                                                              >  1:89993/3‑131 (MQ=255)                                                                                                                                                                                                                                            tgtttttCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAAcc                                                                                                                              <  2:89993/129‑1 (MQ=255)                                                                                                                                                                                                                                               ttttCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACcgcg    <  2:63664/250‑1 (MQ=255)                                                                                                                                                                                                                                                 ttCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAATCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATATGCCGACTGGTGAACACCAACcgcgcg  >  2:258274/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                         GTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCG  >  NC_000913/697059‑697548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑