Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	69,400	G→A	L217L (CTG→TTG)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	69,400	0	G	A	100.0%	80.1 / NA	26	L217L (CTG→TTG)	araB	ribulokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/12);  total (14/12)

AGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAAGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAGGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGC  >  NC_000913/69164‑69615                                                                                                                                                                                                                                             |                                                                                                                                                                                                                        aGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAATCCGCCCCAGCttt                                                                                                                                                                                                           <  1:92519/251‑1 (MQ=255)          aaCTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCcac                                                                                                                                                                                                   <  1:46218/250‑1 (MQ=255)                         gTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATGTATGCCCgg                                                                                                                                                                                   <  1:81336/251‑1 (MQ=255)                           tAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGACATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATTGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGAGGCAAGCCGCCCCAGCTTTCGTGCCACCGAGATTTATGCCCGgcg                                                                                                                                                                                 >  2:17225/1‑251 (MQ=255)                             ggCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCt                                                                                                                                                                               >  1:136332/1‑251 (MQ=255)                                tgtgCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGcc                                                                                                                                                                            >  2:271320/1‑250 (MQ=255)                                  ggcgcctgcgccAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAAc                                                                                                                                                                          <  2:63708/250‑1 (MQ=255)                                      cctgcgccAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTcc                                                                                                                                                                      >  2:191692/1‑251 (MQ=255)                                            acAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGGGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTAATCAAAGAAACTGGCTGGCCGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTCTGCCCGGCGCTGGAACGTCCGcgacc                                                                                                                                                                >  2:145171/2‑250 (MQ=255)                                                       catgtGGCAGGCAAACGCGCCGCCGTAAATCACCACGCGGTCAGGCAGGCCGAGGCGCTGCGTGCATTCCGGGCATAAGGTGCCCACCGGAATATCCGCGGTCGAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATGGGGTCCAGCTCAACAAAGAAACTGGGTGGCGGCTAGCCGCCCCGGCTGTCGTGCCACAGAGAGTTATGCCCGGCGCTGCAACGTCCTCGACGAATATCCTgcg                                                                                                                                                     <  2:161859/247‑1 (MQ=255)                                                         tatGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTgcggg                                                                                                                                                   >  2:102096/1‑251 (MQ=255)                                                              cAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGggt                                                                                                                                              <  1:271320/251‑1 (MQ=255)                                                                     ccgcgcgGCCGGAAATCACCACGGTTTCATGCAGGCCGCGACGCTGCGCCAATTCCGGGCAGTAGTTGCCCACCGGAAGATCTGCATTCGAGGTTGCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAGTAAGCTGGCTGGCGGCAAGCCGCTCCTTGTTGGGTGCCATAGAGGTTGATGCCCGGCGCGGCTACGGCTGTGTCGATTATCCAGCGGGGGGGAGGTACCg                                                                                                                                       <  2:128495/250‑1 (MQ=255)                                                                                    tCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTCTGCCCGGCGCGGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAACGCAGAGCTGGc                                                                                                                        >  2:233659/1‑251 (MQ=255)                                                                                                          gagaCGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATAGCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATcc                                                                                                  <  2:9127/251‑1 (MQ=255)                                                                                                                     cccATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCa                                                                                       <  1:137151/251‑1 (MQ=255)                                                                                                                                        gCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCGGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTCTGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGAGGTACCGGAAAGCAGAGCAGGCACCCAGGCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGt                                                                    >  2:224494/1‑251 (MQ=255)                                                                                                                                         cccACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAACTGGCGATTGAGGATCGGGTCCAGCTCATCAACGAACCTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTc                                                                   >  1:214785/1‑251 (MQ=255)                                                                                                                                                       ggCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACAt                                                     <  1:157619/251‑1 (MQ=255)                                                                                                                                                                ggggtCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCATAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGAttt                                            <  2:246115/248‑1 (MQ=255)                                                                                                                                                                      aGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGTGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGccc                                      >  2:183445/1‑251 (MQ=255)                                                                                                                                                                       gTGAACAGCGGGGAAGTCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCGGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGTAGGATTTTTGCCCa                                     <  2:112200/251‑1 (MQ=255)                                                                                                                                                                            cAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAAcc                                >  1:95784/1‑251 (MQ=255)                                                                                                                                                                                ggggAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGCGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGAGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCAACGCTGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGACCAGAACCATTc                            >  1:231594/1‑251 (MQ=255)                                                                                                                                                                                                 aTTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATaccgc           >  2:210297/1‑249 (MQ=255)                                                                                                                                                                                                          cGGGTCCAGCTCTTCACAGCACCTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGCGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGCGTGGTCCCGGAAAGCAGCGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGCTTTTTGCCCAGAACCATTCGCTGGAATACATACCGCCAATCTAGc  >  2:24817/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                        AGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAAGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAGGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGC  >  NC_000913/69164‑69615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑