Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	69,323	A→G	T242T (ACT→ACC)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	69,323	0	A	G	100.0%	88.0 / NA	26	T242T (ACT→ACC)	araB	ribulokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/14);  total (12/14)

GCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAAGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAGGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACAT  >  NC_000913/69114‑69564                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  gCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAgg                                                                                                                                                                                                            <  2:236589/249‑1 (MQ=255) gCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGa                                                                                                                                                                                                           <  2:95234/250‑1 (MQ=255)        cGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTCTCCGTGAACAGCGGGGACGGCAAATGGCGATTGAGGATCGGGTcc                                                                                                                                                                                                   >  2:10892/1‑251 (MQ=255)                           ggCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAActgg                                                                                                                                                                                >  1:97976/1‑251 (MQ=255)                            gCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAActggc                                                                                                                                                                               <  1:39059/251‑1 (MQ=255)                             cAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAActggct                                                                                                                                                                              <  1:47677/251‑1 (MQ=255)                               aTCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAActagctgg                                                                                                                                                                            >  1:112200/1‑251 (MQ=255)                                                   aGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAATCCGCCCCAGCttt                                                                                                                                                        <  1:92519/251‑1 (MQ=255)                                                            aaCTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCcac                                                                                                                                                <  1:46218/250‑1 (MQ=255)                                                                           gTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATGTATGCCCgg                                                                                                                                <  1:81336/251‑1 (MQ=255)                                                                             tAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGACATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATTGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGAGGCAAGCCGCCCCAGCTTTCGTGCCACCGAGATTTATGCCCGgcg                                                                                                                              >  2:17225/1‑251 (MQ=255)                                                                               ggCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCt                                                                                                                            >  1:136332/1‑251 (MQ=255)                                                                                  tgtgCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGcc                                                                                                                         >  2:271320/1‑250 (MQ=255)                                                                                    ggcgcctgcgccAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAAc                                                                                                                       <  2:63708/250‑1 (MQ=255)                                                                                        cctgcgccAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTcc                                                                                                                   >  2:191692/1‑251 (MQ=255)                                                                                              acAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGGGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTAATCAAAGAAACTGGCTGGCCGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTCTGCCCGGCGCTGGAACGTCCGcgacc                                                                                                             >  2:145171/2‑250 (MQ=255)                                                                                                         catgtGGCAGGCAAACGCGCCGCCGTAAATCACCACGCGGTCAGGCAGGCCGAGGCGCTGCGTGCATTCCGGGCATAAGGTGCCCACCGGAATATCCGCGGTCGAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATGGGGTCCAGCTCAACAAAGAAACTGGGTGGCGGCTAGCCGCCCCGGCTGTCGTGCCACAGAGAGTTATGCCCGGCGCTGCAACGTCCTCGACGAATATCCTgcg                                                                                                  <  2:161859/247‑1 (MQ=255)                                                                                                           tatGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTgcggg                                                                                                >  2:102096/1‑251 (MQ=255)                                                                                                                cAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGggt                                                                                           <  1:271320/251‑1 (MQ=255)                                                                                                                       ccgcgcgGCCGGAAATCACCACGGTTTCATGCAGGCCGCGACGCTGCGCCAATTCCGGGCAGTAGTTGCCCACCGGAAGATCTGCATTCGAGGTTGCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAGTAAGCTGGCTGGCGGCAAGCCGCTCCTTGTTGGGTGCCATAGAGGTTGATGCCCGGCGCGGCTACGGCTGTGTCGATTATCCAGCGGGGGGGAGGTACCg                                                                                    <  2:128495/250‑1 (MQ=255)                                                                                                                                      tCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTCTGCCCGGCGCGGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAACGCAGAGCTGGc                                                                     >  2:233659/1‑251 (MQ=255)                                                                                                                                                            gagaCGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATAGCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATcc                                               <  2:9127/251‑1 (MQ=255)                                                                                                                                                                       cccATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCa                                    <  1:137151/251‑1 (MQ=255)                                                                                                                                                                                          gCCCACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCGGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTCTGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGAGGTACCGGAAAGCAGAGCAGGCACCCAGGCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGt                 >  2:224494/1‑251 (MQ=255)                                                                                                                                                                                           cccACCGGAATATCGGCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAACTGGCGATTGAGGATCGGGTCCAGCTCATCAACGAACCTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTc                >  1:214785/1‑251 (MQ=255)                                                                                                                                                                                                         ggCAGTCCAGGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACAt  <  1:157619/251‑1 (MQ=255)                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  GCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCAGGCAGGCCGAGACGCTGCGCCCATTCCGGGCATAAGGTGCCCACCGGAATATCGGCAGTCCAAGTGTCAGTGAACAGCGGGGAAGGCAAATGGCGATTGAGGATCGGGTCCAGCTCATCAAAGAAACTGGCTGGCGGCAGGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACAT  >  NC_000913/69114‑69564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑