Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,665	T→C	A372A (GCA→GCG)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,665	0	T	C	100.0%	105.3 / NA	33	A372A (GCA→GCG)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/17);  total (16/17)

CTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCA  >  NC_000913/64432‑64903                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                cTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGAACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGatccccc                                                                                                                                                                                                                                >  1:178058/1‑245 (MQ=255)            aTCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCATGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGCGATGACCAAACGCCGCCACCGATCCCCCGTGGCgggct                                                                                                                                                                                                                      >  1:75493/1‑247 (MQ=255)              cagtcTGCGCCATGCCTACCAACAGCCCGACGGGCTCACTCAGAAAGGTGCGGATGCTAGACGGGTACAGGCTTTTATAGTCCCGCACCAGTACCGAATCATCCAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATCGCGATGACCAAACGCAGCCACCGATCCCCCgttgcggtcaacc                                                                                                                                                                                                                   >  2:239723/5‑240 (MQ=255)                          tGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTc                                                                                                                                                                                                       >  1:191660/1‑251 (MQ=255)                                ccaccaGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGtc                                                                                                                                                                                                 <  1:164700/251‑2 (MQ=255)                                                               gCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCa                                                                                                                                                                   <  1:7183/250‑1 (MQ=255)                                                                         taCGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCTAGGAAAAATGGCATGATTTTAGTTTTATggt                                                                                                                                                         >  2:35623/2‑249 (MQ=255)                                                                                        ttATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCa                                                                                                                                          <  1:254165/250‑1 (MQ=255)                                                                                           taGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGGAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTc                                                                                                                                      >  2:90426/1‑251 (MQ=255)                                                                                               ccagcaccagtaCCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCCCTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCa                                                                                                                                   <  2:212021/250‑1 (MQ=255)                                                                                                cagcacaagtacCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCAGACAGGGCTGACGTGTGGCGGCACTTCGCCGAGCTTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGCTGACCAAACGCCGCCACCGATCACCCGTGGCGGTATACCGGCAGGCATGTCACAGTCGCTCGTTCGCGTTAATATGGCATGTTTTTAGTTTTTTGGAAAATCCACGTCCCCAGcgcacaa                                                                                                                                  >  1:249418/1‑244 (MQ=255)                                                                                                   caccagtacCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCtt                                                                                                                              <  1:39194/251‑1 (MQ=255)                                                                                                      cagtaccgAATCATAAAGCCCTGGCCGTGAATCCAGCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCa                                                                                                                           <  1:26429/244‑1 (MQ=255)                                                                                                           ccgAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCTCCAGGGCTGTCGTGTGTCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGATAATACCAAACGCCGCCACTGATCCCCCTTGGCTGTCTACCGTCAGGCCGTTCACCGTCGCTCTTTCGAGTAAAAATGGCATGTTTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTATCAGGTta                                                                                                                      <  1:172065/249‑1 (MQ=255)                                                                                                                tCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCAACAGCTCGCAATCTTTCAGGTTATAAGt                                                                                                                 >  2:231923/1‑251 (MQ=255)                                                                                                                 aagaaagaccgggCCGTGAATCCGTCACGTAGCCGCCAGAGCTGGCGTGTGGAGGCACTTCGCCGAGATTAGGCGCGACATAGCCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCAACGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCAACGCCGCTCGTTCGAGTAAAAAGGGCATGATTGCAGTTTTATGGAAAAGCTGCGTCACCAGCTCGCAATCCTTCAGGTTATAAGt                                                                                                                 <  2:73763/239‑1 (MQ=255)                                                                                                                      aGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAg                                                                                                           <  1:129805/251‑1 (MQ=255)                                                                                                                                                 ccgccAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGATCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAAcggcg                                                                                >  2:120790/1‑251 (MQ=255)                                                                                                                                                          atGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCCGTCACCGTCGCGCGTTCGAGGAAAAAAGGCATGATTTCAGGTTTATGGGAAATCTGCTTCACCAGCTCGCAATCTTTCAGGTTCTAAGTCGCCAGCGCCGGTGTATCTTCGGAGAAACGGCGGTACAAttt                                                                       >  2:208897/2‑251 (MQ=255)                                                                                                                                                                    gcggcACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGa                                                            <  1:231923/251‑1 (MQ=255)                                                                                                                                                                                   gaTTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCg                                             <  1:203293/251‑1 (MQ=255)                                                                                                                                                                                       aGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAg                                         <  1:246336/251‑1 (MQ=255)                                                                                                                                                                                           gcgACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAAATTCGGCCATTCGATCCCACGGGGTTTCGATaacatt                                     >  2:220305/1‑246 (MQ=255)                                                                                                                                                                                             gACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTc                                   <  2:52046/251‑1 (MQ=255)                                                                                                                                                                                              aCATAACCAGCGCGGTGCATACGAGGGAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCGCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTGTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTGCGTCCATTCGATCCCACGGGTTATCGATAGATTTTcc                                  <  2:94827/251‑1 (MQ=255)                                                                                                                                                                                              aCATAACAAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGACAATCTGCGTCACCAGCTCGCACTCTTTCAGGTTATCAGTAGCCCGCGCAGGTTTATCTTCGGCGAAACGGCGGG‑CCATTTCGTCCATTTTATCCCACGGGGTATCTATAGATTTTcc                                  >  2:53938/1‑251 (MQ=255)                                                                                                                                                                                                 tAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCCTTCGATCCCACGGGTTATCGATAGATTTTCCTTc                               >  1:151641/1‑251 (MQ=255)                                                                                                                                                                                                        cgcgGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAAt                        <  1:214615/251‑1 (MQ=255)                                                                                                                                                                                                        cgcgGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGGTATCGATAGATTTTCCTTCTCCTAAt                        >  1:82465/1‑251 (MQ=255)                                                                                                                                                                                                                    gaggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑TAATTTCGTCCATTCGATCCCACGGGTTATCGATAGAATTTCCTTCGCCTACTAACTCCTGAGCg            >  2:90344/3‑251 (MQ=255)                                                                                                                                                                                                                           aTAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGAcgggtt     >  1:226213/1‑246 (MQ=255)                                                                                                                                                                                                                             agagATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTc    <  1:148857/250‑1 (MQ=255)                                                                                                                                                                                                                              gagaTGACCAAACGCCGACACCGATTCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTAGATCGGTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCGTCGGCGAAACGGCGGT‑CGATTGCGTCCATTCGATCCCACGTGTGATCGATAGATTTTCCTTCGCCTAATAACGCCTGAGCgcagggttcta  <  2:178985/251‑11 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                CTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCA  >  NC_000913/64432‑64903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑