Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	67,798	T→A	R180R (CGA→CGT)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	67,798	0	T	A	95.7%	70.9 / NA	23	R180R (CGA→CGT)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (16/6);  minor base G (0/1);  total (16/7)
Fisher's exact test for biased strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACG  >  NC_000913/67558‑68042                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     cTCAATACGCGCCGCTTCTAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTAGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACtt                                                                                                                                                                                                                                            >  2:167210/1‑251 (MQ=255)      tACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTc                                                                                                                                                                                                                                          >  1:247833/1‑248 (MQ=255)                 tCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGGGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATc                                                                                                                                                                                                                            >  1:30370/1‑251 (MQ=255)                       aCGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGttt                                                                                                                                                                                                                      >  1:267358/1‑251 (MQ=255)                                                                cATGGTGTAGCGGCTTCCGTACTCACCGCCCAGCGCGTAAACAGCGCGGTCGCTGATGGAGTCCACCACCTGCACCAGATCGCCACCCCCCCAGGTATTGAGGGAAAAACCGAACTTGATTTGTGCGGCAACTTTACCCCCATGGGGTG‑CCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATgg                                                                                                                                                                              <  1:35738/250‑1 (MQ=255)                                                                   ggTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCtt                                                                                                                                                                          >  1:157815/1‑251 (MQ=255)                                                                                            aCCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAAc                                                                                                                                                 >  1:7683/1‑251 (MQ=255)                                                                                                gcgcGTTAACATCGCCGTCGCTGATGGAGTTCCCCCCCTGCCCCAGACCGCCAACCCCCCAGTTATTGAGGGAGAAACCGAACTTGATCTGTGCGGCAACTTTACCCCCATCGG‑GGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAacgacg                                                                                                                                             <  1:266446/251‑1 (MQ=255)                                                                                                       aaCATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGtt                                                                                                                                      >  1:164562/1‑251 (MQ=255)                                                                                                       aaCATCGCCGTCGCTGATAACGTCCACCACCTGCAGCAGATCGCCAACCGAACAGGTATTGACGGAGACACCGAACTTGATCCGTGCGGCAACTTTATCACCATCGG‑TGCCCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGCCGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGCCTTGTTTATCCTGCCAGTGACTGCTAACGACGGCCTGtt                                                                                                                                      >  2:165524/1‑251 (MQ=255)                                                                                                           tCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTg                                                                                                                                  >  1:97540/1‑251 (MQ=255)                                                                                                              ccGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGttt                                                                                                                                                                      >  2:36410/1‑212 (MQ=255)                                                                                                              ccGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACg                                                                                                                               >  1:176291/1‑251 (MQ=255)                                                                                                                    gggagggAGTCCACCACCTCCCCCAGACCGCCAACCGCCCAGGTATTGAGGGAGAAACCGAACTTGATCTGTGCGGCAACTTTTTCACCATCGT‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATAcg                                                                                                                         <  2:265479/246‑1 (MQ=255)                                                                                                                                                             ggTATTGACGGAGAAACCGAACTTGCTCTGTGCGGCAACGTTATCACAATCGG‑AGACCGCCACTTAACGCATGTTATCGCCCAAACGGCAGACTTTCAGATGAAGGGTATCCTGTTTAGCCACCGCCTGACGCATACAGGAGCAGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCt                                                                                >  1:263205/1‑251 (MQ=255)                                                                                                                                                                     cGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTggttcagg                                                                        >  2:192663/1‑251 (MQ=255)                                                                                                                                                                         gaAACCGCACTTGATCCGTGAGGCAACTTTATCACCCTCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGCATCATGTTTAGCAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCAGAACTCGCGACCGCCATGTGCAGTCTggttcaggttc                                                                     >  2:157240/1‑250 (MQ=255)                                                                                                                                                                                                     tttaccccctccgtgaaCCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCAc                                          <  1:197152/234‑1 (MQ=255)                                                                                                                                                                                                                       cGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGcc                                                                                                                                                                                                            >  2:74987/1‑70 (MQ=255)                                                                                                                                                                                                                            cTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGgtg                  >  2:216802/1‑251 (MQ=255)                                                                                                                                                                                                                                    aTGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTg          <  1:237129/251‑1 (MQ=255)                                                                                                                                                                                                                                         aTCGCCAAAACGGCAGACTTTCAGATGACTGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTTTCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTgcagc      <  2:238944/250‑1 (MQ=255)                                                                                                                                                                                                                                            gCCAAAGCGGCAGACTTTCAGATGACGGGTATCCTGTTAAGAAACCGCCTGATGCATCCAGGAGCCGATACTCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGGCCGCCATGTGCAGTCTGGTTCAGTTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACg  <  2:190014/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     CTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACG  >  NC_000913/67558‑68042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑