Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	67,759	G→A	G193G (GGC→GGT)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	67,759	0	G	A	89.5%	46.4 / ‑5.4	19	G193G (GGC→GGT)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (16/1);  minor base C (0/2);  total (16/3)
Fisher's exact test for biased strand distribution p-value = 1.75e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTC  >  NC_000913/67530‑67975                                                                                                                                                                                                                                      |                                                                                                                                                                                                                          cTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATATGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTc                                                                                                                                                                                                      >  1:20314/1‑250 (MQ=255)       ccAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATg                                                                                                                                                                                               >  1:120297/1‑251 (MQ=255)                             cTCAATACGCGCCGCTTCTAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTAGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACtt                                                                                                                                                                         >  2:167210/1‑251 (MQ=255)                                  tACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTc                                                                                                                                                                       >  1:247833/1‑248 (MQ=255)                                             tCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGGGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATc                                                                                                                                                         >  1:30370/1‑251 (MQ=255)                                                   aCGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGttt                                                                                                                                                   >  1:267358/1‑251 (MQ=255)                                                                                            cATGGTGTAGCGGCTTCCGTACTCACCGCCCAGCGCGTAAACAGCGCGGTCGCTGATGGAGTCCACCACCTGCACCAGATCGCCACCCCCCCAGGTATTGAGGGAAAAACCGAACTTGATTTGTGCGGCAACTTTACCCCCATGGGGTG‑CCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATgg                                                                                                           <  1:35738/250‑1 (MQ=255)                                                                                               ggTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCtt                                                                                                       >  1:157815/1‑251 (MQ=255)                                                                                                                        aCCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAAc                                                                              >  1:7683/1‑251 (MQ=255)                                                                                                                            gcgcGTTAACATCGCCGTCGCTGATGGAGTTCCCCCCCTGCCCCAGACCGCCAACCCCCCAGTTATTGAGGGAGAAACCGAACTTGATCTGTGCGGCAACTTTACCCCCATCGG‑GGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAacgacg                                                                          <  1:266446/251‑1 (MQ=255)                                                                                                                                   aaCATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGtt                                                                   >  1:164562/1‑251 (MQ=255)                                                                                                                                   aaCATCGCCGTCGCTGATAACGTCCACCACCTGCAGCAGATCGCCAACCGAACAGGTATTGACGGAGACACCGAACTTGATCCGTGCGGCAACTTTATCACCATCGG‑TGCCCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGCCGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGCCTTGTTTATCCTGCCAGTGACTGCTAACGACGGCCTGtt                                                                   >  2:165524/1‑251 (MQ=255)                                                                                                                                       tCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTg                                                               >  1:97540/1‑251 (MQ=255)                                                                                                                                          ccGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGttt                                                                                                   >  2:36410/1‑212 (MQ=255)                                                                                                                                          ccGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACg                                                            >  1:176291/1‑251 (MQ=255)                                                                                                                                                gggagggAGTCCACCACCTCCCCCAGACCGCCAACCGCCCAGGTATTGAGGGAGAAACCGAACTTGATCTGTGCGGCAACTTTTTCACCATCGT‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATAcg                                                      <  2:265479/246‑1 (MQ=255)                                                                                                                                                                                         ggTATTGACGGAGAAACCGAACTTGCTCTGTGCGGCAACGTTATCACAATCGG‑AGACCGCCACTTAACGCATGTTATCGCCCAAACGGCAGACTTTCAGATGAAGGGTATCCTGTTTAGCCACCGCCTGACGCATACAGGAGCAGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCt             >  1:263205/1‑251 (MQ=255)                                                                                                                                                                                                 cGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTggttcagg     >  2:192663/1‑251 (MQ=255)                                                                                                                                                                                                     gaAACCGCACTTGATCCGTGAGGCAACTTTATCACCCTCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGCATCATGTTTAGCAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCAGAACTCGCGACCGCCATGTGCAGTCTggttcaggttc  >  2:157240/1‑250 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                          CTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGG‑TGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTC  >  NC_000913/67530‑67975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑