Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,847	C→T	G497G (GGG→GGA)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,847	0	C	T	97.4%	113.9 / NA	39	G497G (GGG→GGA)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (20/18);  minor base G (0/1);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCG‑CAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGTGTGTCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGG  >  NC_000913/66600‑67085                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               gCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCg                                                                                                                                                                                                                                             <  1:94491/251‑1 (MQ=18)       tgtAGGCATCATAAGACGCGACAGCGTCGCATCAGGAGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGCA‑CGCGTAGGCCTGATAAGACGAGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTCTCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGACGCGACTACTTAGCGACGAAATCCGtactac                                                                                                                                                                                                                                       >  2:155405/1‑251 (MQ=17)          aGGCCGGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTTCTTATCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACt                                                                                                                                                                                                                                    <  2:238420/251‑1 (MQ=18)                                       cAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACCAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTCCTTAGCGACGAAATCCGTACTACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGc                                                                                                                                                                                                       >  2:87578/1‑251 (MQ=18)                                            gTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCa                                                                                                                                                                                                  <  1:100926/251‑1 (MQ=18)                                             ttGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGc                                                                                                                                                                                                   >  1:264284/1‑249 (MQ=18)                                                        aTGCGCTTTGCTTAGCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGGGGCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCGTATCCGGCCTACAAAATCGCAGCGTGTAGTCCGGATAAGACGCGTGAGCGTCGGATTTGGCGTGACACGTCTGATGGGACTGCGTAGCGGCTAAAGCCGTAATACACTTCGTTCCAGGGCAGCTCGTCTTTAAACGCTGGCAGGCGAGTATCGt                                                                                                                                                                                      <  1:117916/251‑1 (MQ=9)                                                           cgcTTTGCTTATCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTat                                                                                                                                                                                   <  1:81833/251‑1 (MQ=18)                                                           cgcTTTGCTTATCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTat                                                                                                                                                                                   <  1:39232/251‑1 (MQ=18)                                                                tGCTTATCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACTAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTatcaat                                                                                                                                                                               <  1:52974/250‑1 (MQ=18)                                                                 gCTTATCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTTTCGTTatcaatc                                                                                                                                                                              <  1:197384/250‑1 (MQ=21)                                                                 gCTTATCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTatcaatc                                                                                                                                                                              <  1:197375/250‑1 (MQ=21)                                                                 gCTTATCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTatcaatc                                                                                                                                                                              <  1:2129/250‑1 (MQ=21)                                                                     aTCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGt                                                                                                                                                                         <  1:92862/251‑1 (MQ=21)                                                                           ccTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTc                                                                                                                                                                   >  1:234925/1‑251 (MQ=21)                                                                                   aatcggaacgcgTAGTCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGTTTTCACACACCCTGATTTCAAAGTCg                                                                                                                                                            >  2:30473/9‑251 (MQ=18)                                                                                       ggaacgcgTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCa                                                                                                                                                        <  2:173871/247‑1 (MQ=25)                                                                                         aacgcgTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGGTATCAATACCCGTGATTTCCATGTCGTGCAtc                                                                                                                                                      >  2:191711/3‑251 (MQ=21)                                                                                                         aaGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGgcgcc                                                                                                                                      >  1:38968/1‑250 (MQ=25)                                                                                                                     gggcGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGt                                                                                                                          <  2:63032/248‑1 (MQ=37)                                                                                                                              aGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAt                                                                                                                 >  1:68224/1‑251 (MQ=37)                                                                                                                               ggCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGTTCGTTATCAATCACCGTGGTTTCAATTTCTTGCATCTCGGCGAACTGGCGCATCTCGTTGAGGtttcacgcgtg                                                                                                                >  2:229514/1‑242 (MQ=25)                                                                                                                                                 cgcTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTg                                                                                              <  1:186871/251‑1 (MQ=38)                                                                                                                                                               ggCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGAGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGAGCGTCTTTAAACGCTGGCAGGCGGGTCTCGTTTTCAATCAACGTGATTTCAATGTCGTGCATCTCGGCGAAATGGCGGATATCGTTGAGGTTCATCGCATGGCTGAAGACGGGATGGTGCTCGcccacagcg                                                                                 >  1:176822/1‑243 (MQ=255)                                                                                                                                                                gCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGGTATCAATCACCGGGATTTTAATGTCGTGCATCTCGGCGAACTGGCGCATATCGGTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCg                                                                                 >  1:2281/1‑249 (MQ=255)                                                                                                                                                                 ccTACAAAATCGAAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCGTCAGGCGTTACACACCTGCTGCGACTACTTCGCTACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTTTCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCTCCAGCGAgg                                                                              <  2:138811/251‑1 (MQ=255)                                                                                                                                                                    aCAAAATCGCAGCGGGTAGGCCTGATAAGACGAGTCAGGGTCGCATCAGGCGTTACACTCCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGAt                                                                            <  2:266035/250‑1 (MQ=255)                                                                                                                                                                     cAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATcc                                                                          <  1:257736/251‑1 (MQ=255)                                                                                                                                                                       aaaTCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCACCAGCGAGGATCCAc                                                                        >  1:247413/1‑251 (MQ=255)                                                                                                                                                                                      ggCCTGATAAGACTCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTCCTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTCTCGTTATCAATCTCCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGtt                                                         <  2:58577/251‑1 (MQ=255)                                                                                                                                                                                         ctgATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGGGATTTCAATGTCGTGCATCTCGGCGAACTTGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGGATGGGGCGCGCCACCAGCGAGGATCCAAGCCTCGGAAGCAGTTggg                                                      >  2:33693/3‑250 (MQ=255)                                                                                                                                                                                          tgATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGGTGGc                                                      >  1:35576/2‑250 (MQ=255)                                                                                                                                                                                           gATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGGTCCGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGc                                                      >  2:155671/1‑249 (MQ=255)                                                                                                                                                                                            aTAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGCTCGTTATCAATCACCGCGATTTCAATGTCGTGCATCCCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCACCCGCGAGGATCCACGCTTCGGAAGCAGTTGGc                                                      >  1:168980/1‑248 (MQ=255)                                                                                                                                                                                                                  cAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAgcg                             <  2:234925/251‑1 (MQ=255)                                                                                                                                                                                                                        ttacacaactgATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCAACTCGGCGAACTGGCGCATATCGTTGAGGGTCAGCGCACGGCTGAAGACGGGTTGGGGGGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGAGCCGGGTGCGCGTTCCACAGCGCATTCg                       >  2:31711/11‑251 (MQ=255)                                                                                                                                                                                                                         tACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGGGATTTCAAAGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCCGTTGGCAGATCCGGTTGCGCCTTCCCCCGCGCATTCGc                      >  1:151024/1‑251 (MQ=255)                                                                                                                                                                                                                            acacctgATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGTTATCAAACACCGTGATTTCAATGTCGGGCATCTCGGCGAACTGGCGCCTATCGTTGCGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCAc                   >  1:133775/7‑251 (MQ=255)                                                                                                                                                                                                                                             ttAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGGTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGGTGCGCTTTCCACCGCGCATTCGCCACCGGCAGGTTCGGCAggg  >  1:184719/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               GCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCG‑CAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGTGTGTCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGG  >  NC_000913/66600‑67085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑