Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,518	T→C	E11E (GAA→GAG)	araD ←	L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,518	0	T	C	100.0%	114.2 / NA	33	E11E (GAA→GAG)	araD	L‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/13);  total (20/13)

CCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCC  >  NC_000913/66276‑66760                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   ccgccAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATAc                                                                                                                                                                                                                                            >  2:186871/1‑251 (MQ=255)  cgccAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGGTATGTTGTGGCAGCGCCAGGTTGGCCTCTAATAcc                                                                                                                                                                                                                                           >  2:78764/1‑251 (MQ=255)                 aTGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGAtct                                                                                                                                                                                                                            <  1:172265/251‑1 (MQ=255)                       gATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATAGCGTCAGCGGTCATGATGCTGTAATCGACGCCTGAAGGTTTTATCACAAAGACGCCGCGCTCGCGAGCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTGTTGGCAGCGCCAGGTTGGCCTCGAATACCTGGCGTTTGAGATCTTCTaa                                                                                                                                                                                                                       <  1:116149/250‑1 (MQ=255)                               agtcGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGAc                                                                                                                                                                                                              >  1:48245/4‑251 (MQ=255)                                              gtatCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGCGATCTTCTAACATGTTGACTCCTTCGTGCCGGAt                                                                                                                                                                                               >  1:138811/4‑251 (MQ=255)                                                             tttttCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGTCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCTTGCCGGATGCGCTTTGCTTATcc                                                                                                                                                                                >  2:197384/1‑251 (MQ=255)                                                             tttttCGCACCTTCAACCACTTCACCGGCTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCAAATAACAGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTCCCGCATGCGCTTTGCTTATcc                                                                                                                                                                                >  2:197375/1‑251 (MQ=255)                                                                  cgcaacTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGCTGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGCCGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCCGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGCTGCGCTTTGCTTATCCGGCCt                                                                                                                                                                           >  2:108188/6‑251 (MQ=255)                                                                    ccccTTCATCCACTTCTCCGTTTTCGTTGCTCCCCCCGACCATATCGTCATCTTTCCTGATGCTGTAATCGCCGCCTTAAGGTTTGATCACAATGCCGCCGCGCTCGCGATCTACGTCTCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACGCCTGCGTGCCGGATGCGCTTTGCTTATCCGGCCTAc                                                                                                                                                                         <  2:122097/249‑1 (MQ=255)                                                                     accatCAACCACTTCACCGTTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTGTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTATAACATTTTGACTCCTTCTTGCCGGATGCGCTTTGCTTATCCTGCCTACa                                                                                                                                                                        <  1:92029/247‑1 (MQ=255)                                                                      ccTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACaa                                                                                                                                                                       <  1:35141/251‑1 (MQ=255)                                                                                        gTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGcc                                                                                                                                                     >  1:241638/1‑251 (MQ=255)                                                                                                  tAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGAcg                                                                                                                                           >  1:85272/1‑251 (MQ=255)                                                                                                       aCGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGcc                                                                                                                                       >  2:100926/1‑250 (MQ=255)                                                                                                         gACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATTTTGACTCCTTCGTGCCGGATGCGCTTTGCGTATCCGGCCGACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGc                                                                                                                                    <  1:175702/251‑1 (MQ=255)                                                                                                         gACCATATCGTCAGCGGTCATGATGATGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGc                                                                                                                                    >  1:87236/1‑251 (MQ=255)                                                                                                            cATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATCCGCTTTGCTTATCCGGCCTACAAAATCGCCTAGTGCAGGCCTGATCAGCCTCGCCAGCgcc                                                                                                                                 >  2:206057/1‑249 (MQ=255)                                                                                                                               atgCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGAGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGcc                                                                                                              >  2:257736/3‑251 (MQ=255)                                                                                                                                 gCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCg                                                                                                             <  1:85553/250‑1 (MQ=255)                                                                                                                                    gTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGAt                                                                                                          <  1:166722/250‑1 (MQ=255)                                                                                                                                    gTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGAt                                                                                                          <  2:48245/250‑1 (MQ=255)                                                                                                                                       aTCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATgcgc                                                                                                      >  1:173871/1‑251 (MQ=255)                                                                                                                                                            cacaAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATACGGCCTACaa                                                                                  <  2:9804/250‑1 (MQ=255)                                                                                                                                                                        gcgcTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGt                                                                     <  2:80597/251‑1 (MQ=255)                                                                                                                                                                                 aTCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGGGTAGGCCTGATAAGACGCGCCAGCGTTGCATCAGGCGTTGAAGGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGAt                                                            <  2:115747/251‑1 (MQ=25)                                                                                                                                                                                                               cGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTg                               >  1:29188/1‑250 (MQ=255)                                                                                                                                                                                                                 tGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGACTACAAAATCGGAACGCGTAGGCCTGATACGAAGCGCCAGCGTCGTATCAGGCGTTGAAt                            >  2:68093/1‑251 (MQ=21)                                                                                                                                                                                                                    ccAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGcc                         >  2:31892/1‑251 (MQ=255)                                                                                                                                                                                                                               gttGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCtt               <  1:121547/249‑1 (MQ=255)                                                                                                                                                                                                                                ttGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTg             >  1:63032/1‑251 (MQ=255)                                                                                                                                                                                                                                     aGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTAt        >  1:177361/1‑251 (MQ=255)                                                                                                                                                                                                                                           aGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGcc  >  1:131893/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   CCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCC  >  NC_000913/66276‑66760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑