Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,161,248	G→T	G61V (GGC→GTC)	fabR →	DNA‑binding transcriptional repressor FabR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,161,248	0	G	T	100.0%	91.0 / NA	28	G61V (GGC→GTC)	fabR	DNA‑binding transcriptional repressor FabR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/13);  total (15/13)

TTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTC  >  NC_000913/4161009‑4161484                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             ttGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTccc                                                                                                                                                                                                                                    >  1:107201/1‑250 (MQ=255)           tatGATTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGCTGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGACGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGATCCCACCTCtttttt                                                                                                                                                                                                                         >  2:173013/1‑250 (MQ=255)            atGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTAt                                                                                                                                                                                                                        >  1:78997/1‑251 (MQ=255)              tttttATTGCGTTACCGTTCATTCAAAATACTGGAGCAATCCAGTATGTTCATTCTCGGGTATAGTGCCAGCAGTACTTTTGGAAGGGATTCAGACATCGGGATGGGCGAAAGAGCGAAAAAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATc                                                                                                                                                                                                                       <  1:88961/249‑1 (MQ=255)               ttttATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCgg                                                                                                                                                                                                                     >  1:14920/1‑251 (MQ=255)                tttATTGCGTTCCCGTTCATTCACAATACTGGATCAATCCAGTATGTTCATTCTCTGGTATAGTCCCAGCAGTACTTTGGGCAAGGATGCAGACATCGTGATGGGCGTAAGAGCGCAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCgg                                                                                                                                                                                                                     <  1:250911/250‑1 (MQ=255)                          tACCGTTCATTCACAATCCTGGAGCAATCCAGTATTTTCATTCTCTGTTATAGTGCCAGCAGTATTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAAAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCcgcg                                                                                                                                                                                                           <  1:260799/250‑1 (MQ=255)                           aCCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGAc                                                                                                                                                                                                         >  1:201396/1‑251 (MQ=255)                                             gggAGCAAGCCAGTATGTTCATTCGCGGTTAGAGTGCCAGCAGTACTTTGGGCAAGGATTCAGACATCGTGAGGGGAGAAAGACCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTg                                                                                                                                                                                       <  1:37878/250‑1 (MQ=255)                                                      ccAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGtt                                                                                                                                                                              >  1:90804/1‑251 (MQ=255)                                                                                   agcagTACTTTTGGAAAGGATTCAGACATCGTGAGGGGCGTAAGAGCGCAAAAAAAAAAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACt                                                                                                                                                 <  1:223061/251‑1 (MQ=255)                                                                                    gaagTACTTTTGGAAAGGATTCAGACATCGTGATGGGCGAAAGAGCGCAAAAAAAAAAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTc                                                                                                                                                <  2:107201/249‑1 (MQ=255)                                                                                      agTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACTACAAGCAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTTGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGAAGAAGACGACCTGGGTCTGACCCTGGGTGGTGAGAGCGGTTTAATGCTACGCCaaaccat                                                                                                                                              >  2:232117/1‑246 (MQ=255)                                                                                      agTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCAt                                                                                                                                              >  1:56543/1‑251 (MQ=255)                                                                                                                  tGATGGGCGTAAGAGCGCAACACAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCaa                                                                                                                   >  2:100220/1‑250 (MQ=255)                                                                                                                     gtgctctcccgaacaaaaaaaaaGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAgg                                                                                                                <  1:265703/232‑1 (MQ=255)                                                                                                                                  acaaaaaaaaaaaaaACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATcc                                                                                                  <  1:234787/246‑1 (MQ=255)                                                                                                                                    aacaaAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAgg                                                                                                                >  2:265703/1‑235 (MQ=255)                                                                                                                                       aaaaaaaaaaACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCTACGTAGACGAACTGGGTCTGACCATGTTTGATGAGAGCGGTTTAATGCTACGCCATCTCATGCGCCAGGCTCGTCATCTTATCGCCAAAGGCGGGAGTGTGATCCGCAcc                                                                                             <  1:245446/251‑1 (MQ=255)                                                                                                                                           aGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCgg                                                                                         >  1:80453/1‑251 (MQ=255)                                                                                                                                                                ggtggAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCa                                                                    <  1:226631/251‑1 (MQ=255)                                                                                                                                                                            aTTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATc                                                        <  2:33322/251‑1 (MQ=255)                                                                                                                                                                                                    cAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGAGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCAAACTCATGAGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGCGTTCATCGGTCATAATCCTAACGCCTTTCGGTTATTATTGc                                >  1:118804/1‑251 (MQ=255)                                                                                                                                                                                                                ccTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTCTTATTGCGGGAACGCTCCg                    >  2:162253/1‑251 (MQ=255)                                                                                                                                                                                                                  tGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGGGATCCGCACCTCGGTCTCCACATTTATGGGGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTTTTGCGGGAACGCTCCgcc                  >  2:161844/1‑249 (MQ=255)                                                                                                                                                                                                                        tGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTc             <  2:201396/250‑1 (MQ=255)                                                                                                                                                                                                                            tGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTg        >  1:95085/1‑251 (MQ=255)                                                                                                                                                                                                                                  ggCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTc  <  2:268144/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             TTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTC  >  NC_000913/4161009‑4161484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑