Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,993,531	T→C	R793R (CGT→CGC)	cyaA →	adenylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,993,531	0	T	C	100.0%	116.8 / NA	34	R793R (CGT→CGC)	cyaA	adenylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/16);  total (18/16)

GCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTG  >  NC_000913/3993285‑3993771                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 gCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGGGGGCGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGGGGGATATCACCACCGCGAAGGCAGCAAAGAGGAGCTGGGACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGctttt                                                                                                                                                                                                                                              >  2:108414/1‑250 (MQ=255)                               ggTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAAc                                                                                                                                                                                                                 <  2:258427/250‑1 (MQ=255)                               ggTTGAAACCAATCACGTCAAATTACCGGCGGTGGGGGACGGCTTTGCCAGCGAAGGGGTCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCCGTCGCTTCTACTCGTCATCGCCTGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCCAcc                                                                                                                                                                                                                >  1:258427/1‑251 (MQ=255)                                              cGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATc                                                                                                                                                                                                 <  1:249401/251‑1 (MQ=255)                                               gTCAAATTAACGGCGGTGGTGGGCGGCTTTTCCAGCGGAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCCTTAATATCTACATTCTTGGCGAAAGCAACCGGGTTGGGGGATATCACCACTGCGAAGGCCGCAAAAAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCACACTGCCGCAGTTCttttc                                                                                                                                                                                                >  2:36432/1‑247 (MQ=255)                                                 cAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCTAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCCCCACTTCGAAGTCAGCAAAGAGGAGCTGGTACGTGACGTCATTCTCTTCTACTCGTCATCGCATGCCCGCTTTACCTACGGCTCAAGCTGCATCAACTTCAACCTGCCGCAGTTCTATCAGa                                                                                                                                                                                              <  2:153269/251‑1 (MQ=255)                                                               tggtggACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCGGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGAt                                                                                                                                                                                >  1:250662/1‑251 (MQ=255)                                                                                   gAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGAttcc                                                                                                                                                            >  1:98527/1‑251 (MQ=255)                                                                                   gAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGAttcc                                                                                                                                                            >  1:71163/1‑251 (MQ=255)                                                                                        gATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGAttccgttcc                                                                                                                                                       >  1:231377/1‑251 (MQ=255)                                                                                                       tttCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATc                                                                                                                                         <  1:125897/250‑1 (MQ=255)                                                                                                                      aGACGAGAATGGTTTTAATATCTCCATTCTCGCCGACAGCAACCGGGTTGAGGTATATCACCACTGTGAAGGCACCAAAGAGCCGCTGGTACGTGCCGCCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCTACCTGACGCAGTTCTATCAGATTGTGAAGGCTGATGGTCGTGAACAGGTGATTCCGTTCCGCCCATCATCTATCTGTAACATgctgtctg                                                                                                                         >  2:164234/1‑245 (MQ=255)                                                                                                                       gACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGATGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGc                                                                                                                        >  2:53279/1‑251 (MQ=255)                                                                                                                           tgaATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGTAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAAt                                                                                                                    >  2:78258/2‑251 (MQ=255)                                                                                                                               tGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAgg                                                                                                                >  1:10561/1‑251 (MQ=255)                                                                                                                                   tttAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATc                                                                                                             >  2:249760/1‑250 (MQ=255)                                                                                                                                    ttaaaaaagaaaatctcGACGAAAGCAACCGGGTTGAGGTATATCACAACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTGTGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCAc                                                                                                           >  1:173941/14‑251 (MQ=255)                                                                                                                                     tAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTTCCAATCAGGATCAc                                                                                                           <  1:210122/250‑1 (MQ=255)                                                                                                                                          tCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGTTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATAc                                                                                                      <  2:192836/250‑1 (MQ=255)                                                                                                                                             ccaggctggacgaAAGCAACCGGGTAGAGGTATAGCGCCTCTGCGAAGGCCGCAAAGAGGGGCTGGTACGTGACTTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATAcg                                                                                                     <  2:8010/240‑1 (MQ=255)                                                                                                                                                    cgacgaAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTa                                                                                            <  1:201389/250‑1 (MQ=255)                                                                                                                                                          aaGCAACAGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGGACGTGACGTCAGGCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAAACTGCCGCAGTTCTATCAGATTGTGAAGGAAGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTAGCGGTAACATGCCGCCTGCCAATCAGGATCACGAAACGCCGCTATTACAGCAat                                                                                     >  2:249330/1‑251 (MQ=255)                                                                                                                                                                 cGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTc                                                                              <  2:39174/251‑1 (MQ=255)                                                                                                                                                                 cGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGGACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCTCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTTTTACAGCAATATTTTTc                                                                              <  1:228012/251‑1 (MQ=255)                                                                                                                                                                       gAGGTATATCACCACTGCGAAGGCAGCAAAGAGTAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTCCCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCCCGATACGCCGCTATTACAGCAATATTTTTCGtgatg                                                                        <  1:249330/251‑1 (MQ=255)                                                                                                                                                                                         gAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCACTCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCg                                                        >  1:172709/1‑249 (MQ=255)                                                                                                                                                                                          aaGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCCTGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAgg                                                     >  2:259823/1‑251 (MQ=255)                                                                                                                                                                                          aaGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAgg                                                     >  2:44113/1‑251 (MQ=255)                                                                                                                                                                                                      aggagCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCAt                                         <  1:108414/251‑1 (MQ=255)                                                                                                                                                                                                       ggagCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATg                                        <  2:2796/251‑1 (MQ=255)                                                                                                                                                                                                             ggTACTTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGATTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGGTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTTATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCt                                  <  2:109590/251‑1 (MQ=255)                                                                                                                                                                                                             ggTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCGTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCGCGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCt                                  <  2:173203/251‑1 (MQ=255)                                                                                                                                                                                                                          aGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGca                                                                                                                                                    >  2:169250/1‑124 (MQ=255)                                                                                                                                                                                                                          aGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGca                                                                                                                                                    <  1:169250/124‑1 (MQ=255)                                                                                                                                                                                                                                             cGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCctgctg  >  1:104753/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 GCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTG  >  NC_000913/3993285‑3993771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑