Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,629,921	C→T	E228K (GAG→AAG)	yhiI ←	putative membrane fusion protein YhiI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,629,921	0	C	T	100.0%	109.4 / NA	33	E228K (GAG→AAG)	yhiI	putative membrane fusion protein YhiI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/17);  total (16/17)

GAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTG‑AACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTC‑ACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTCGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATA  >  NC_000913/3629676‑3630162                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  gAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTG‑AACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTC‑ACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCa                                                                                                                                                                                                                                               >  1:73118/1‑250 (MQ=255)                         ggctgttttcaccggttttGGGGTGGAACTGGGCGACACGGCCGACAAACCTGATGTTTGCAGGAATCCGAAGTTCTGGCGCGGCACCGAGGATCAGCCGGGCTTC‑ACCCCCCATTTTCAGCGTGCCCGCCTGGTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGcgtccgtc                                                                                                                                                                                                                        <  2:19279/235‑1 (MQ=255)                              tttCGACGGTTTTTGGCGTG‑AACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTC‑ACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCggg                                                                                                                                                                                                                 >  1:57581/1‑251 (MQ=255)                                gcGACGGTTGTGGGCTTG‑AACTGGGGGACCCTGGCGAAAAAACTGATGGTTGAAGGAATACGCAGATCTGGCGCGGCATGGAGGATCCGCCGTGCTTC‑CCCGCCCGGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCTCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCTTCACGCgggg                                                                                                                                                                                                                <  2:255684/249‑1 (MQ=255)                                 cgccgtgttttGGCGTG‑ACCTGGGCGACACGGGCGACAAACCTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGACCAGCCGGGTGTC‑ACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGc                                                                                                                                                                                                               <  1:230627/243‑1 (MQ=255)                                  gACGGTTTTTGGCGTG‑AACTGGGCGACACTGGCGACAACACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTC‑ACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCtt                                                                                                                                                                                                             >  2:240559/1‑251 (MQ=255)                                   aCGGTTTTTGGCGTG‑AACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTC‑ACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCttt                                                                                                                                                                                                            >  1:103365/1‑251 (MQ=255)                                                   aaCTGGGCGACACGGGCGACAAAACTGGTGGTTGCAGGAATCCGCAGATCTGGCGCGGCTGCGAGGATCAGCCGGGCTTC‑ACCGCCCAGTTTCAGCGTTCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGGCACGCGGGGCTTTCAGTTCGCTGTCATc                                                                                                                                                                                             <  1:226075/251‑1 (MQ=255)                                                      ggggcgaaccggggaaCAAAACTGATGGTTGAAGAAATCCGCAGATCGGGCGCGGCATCGGGGTTCAGCCGGGCTTC‑CCCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGat                                                                                                                                                                                          <  2:73118/236‑1 (MQ=255)                                                                              aTGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTC‑ACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGt                                                                                                                                                                  <  1:95373/251‑1 (MQ=255)                                                                                      gggAATACGCAGATCTGGCGCGGCATCGAGGATCGGCCGGGCTTC‑ACCGCCCGGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTtgtg                                                                                                                                                          <  2:118478/250‑1 (MQ=255)                                                                                            agcaaGATCTGGCGGGGCATCGAGTATCAGCCGG‑CTTCCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCtt                                                                                                                                                    <  1:50866/247‑1 (MQ=255)                                                                                            actcAGATATGTCGCGGCATCGAGGATCCGCCCGGATTC‑ACCGCCCAGTTTCAGCGGGCCCGCCTGTCCGGGTGGCAGGAAGAAAGTCATATAGACGTCGCTGCCATCGACCCTATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGCCATCGATATCTGCGGCCATGCGCAGTTCAGtcgcatctgtcgcct                                                                                                                                                    >  2:262821/4‑237 (MQ=255)                                                                                                 gATCTGGCGCGGCATCGAGGATCAGCCGGGCTTC‑ACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTTTGCCGCTTCGAcc                                                                                                                                               >  2:184628/1‑250 (MQ=255)                                                                                                      ggCGCGGCATCGAGGATCAGCCGGGCTTC‑CCCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGAcgcg                                                                                                                                             <  1:103743/248‑1 (MQ=255)                                                                                                         gcgggagcgggtctCAGCGGGGCTTC‑ACCGCCCAGTTTCGGGGGGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTg                                                                                                                                       <  1:153622/238‑1 (MQ=255)                                                                                                                cGAGGATCAGCCGGGCTTC‑ACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGcccgg                                                                                                                                >  2:20715/1‑248 (MQ=255)                                                                                                                        ggCCGGGCTTC‑ACCGCCAAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTg                                                                                                                                                                                     <  2:251787/189‑1 (MQ=255)                                                                                                                        aGCCGGGCTTC‑ACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTg                                                                                                                                                                                     >  1:251787/1‑190 (MQ=255)                                                                                                                                 tC‑ACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGgtgcgtg                                                                                                                <  1:104590/250‑1 (MQ=255)                                                                                                                                         ccAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGCATGATATTGGTGCGTGCCGCTTCt                                                                                                        >  1:44555/1‑251 (MQ=255)                                                                                                                                                 gggttgcacGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGAGGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTTGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGTCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGc                                                                                                <  2:65011/243‑1 (MQ=255)                                                                                                                                                 aGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGAGTGCCGCTTCTTTAGCCGc                                                                                                >  1:57388/1‑251 (MQ=255)                                                                                                                                                 aGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGGGCGTGCCGCTTCTATAGCCGc                                                                                                >  1:162686/1‑251 (MQ=255)                                                                                                                                                                 cGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGCCCGCCTGCCGCCAGCACTTCGCCTGGATTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCCTCGACGCGGGTTTTGGCCTGAATGATTTTGGGGGGTGGCGCTTTCATAGCCACTTTGGAAACCgctctc                                                                                >  2:189944/1‑246 (MQ=255)                                                                                                                                                                    ttGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTg                                                                              >  1:121886/1‑250 (MQ=255)                                                                                                                                                                                       ataGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCCCTTTAGAAGCCGACACCTTCGCTTTCGCCGATTcccacc                                                          >  2:89758/1‑247 (MQ=255)                                                                                                                                                                                                        cgcccATATCCAGCCCCCGCCCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTCCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGATTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCa                                         <  2:57581/248‑1 (MQ=255)                                                                                                                                                                                                             atatTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGc                                                                                                <  2:201426/191‑1 (MQ=255)                                                                                                                                                                                                             atatTCAGCACCAGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGc                                                                                                >  1:201426/1‑191 (MQ=255)                                                                                                                                                                                                                   aGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTTGACGCGGGGTTGCGCCTGAATGATATTGGGGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCCCTCAGCGGCGGcgcg                              >  2:215615/1‑251 (MQ=255)                                                                                                                                                                                                                                         gCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCa        <  1:260562/251‑1 (MQ=255)                                                                                                                                                                                                                                               cGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATa  <  1:20715/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  GAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTG‑AACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTC‑ACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTCGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATA  >  NC_000913/3629676‑3630162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑