Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,811	G→T	intergenic (‑31/+44)	polB ← / ← araD	DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,811	0	G	T	100.0%	106.6 / NA	32	intergenic (‑31/+44)	polB/araD	DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/18);  total (14/18)

CCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACC  >  NC_000913/65567‑66050                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  ccTTTAACGCCAGGGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTTCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGGCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAg                                                                                                                                                                                                                                              <  1:166372/250‑1 (MQ=255)         gCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTc                                                                                                                                                                                                                                     <  1:204193/251‑1 (MQ=255)                gtgtCAGGCGAACGCCTTGTTCCCCCTGCAAAATATGCGGGGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTACCCTGCAACGGCCCGTTGTCCGTCCCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGAt                                                                                                                                                                                                                              <  2:88722/251‑1 (MQ=255)                    cAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACATGATCGGCGGGAATAAACACCACGGACTCTTGCGGTGCAAGCGTAAACTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAAAATTCTGTCCCTTGCGGGGGGTCCCGCCAGTGTCGGGGTAAGATAAA‑ACCTGCCTGCGCCCCGCTGTAATTTCCTCAAAAAACCAAGGTTGATTTTATCCTGGTTTCGTTTGAtttgg                                                                                                                                                                                                                          >  1:159591/1‑248 (MQ=255)                            cgCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGttt                                                                                                                                                                                                                  <  1:34942/250‑1 (MQ=255)                                     cACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTGGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACGTCGGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGTCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTc                                                                                                                                                                                                         <  2:132190/251‑1 (MQ=255)                                                         gcgcGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCGGCAACGGCCCGTGGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGcc                                                                                                                                                                                     <  1:106226/251‑1 (MQ=255)                                                          cgcgGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCt                                                                                                                                                                                    <  1:191241/251‑1 (MQ=255)                                                              gggAACCGGCTCGGCGGGAATAAACGCCACGGACTCTGGCGGTGCAAGCGTAACCTTCACCGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTcgc                                                                                                                                                                                <  2:114530/251‑1 (MQ=255)                                                                                      cGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGttt                                                                                                                                                       >  1:453/1‑251 (MQ=255)                                                                                       cccACGGACTCTTGCGGTGCAAGCGTAACCTGCACCGGCCCGTTGTCCGCCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTa                                                                                                                                                      <  1:52510/250‑1 (MQ=255)                                                                                         cACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTTATTATAGCCTGGTTTCGTTTGATTGGCTGTGGGTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGGTTAtt                                                                                                                                                    >  2:146637/1‑250 (MQ=255)                                                                                                 ctTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCg                                                                                                                                            >  1:58089/1‑251 (MQ=255)                                                                                                          cAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTACGATAAA‑AACTGCCTGCGCCACGCTGTAATTCACTCAAAAAACCCGGCTTGATTATAGCCCGGTTTCGGTTGATTGGCTGTGGTTTTATACCGTCATTAATGCCCCGAATTTGCCTTCGCGGCCAT‑CTTCCGCCAATAGTGtttctttagcagcggtttcctcc                                                                                                                                   >  2:166722/1‑231 (MQ=255)                                                                                                           aaGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGGTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATTCTTACGCCGATTGTGTTTATTTAGCAGCGTTTGCTGCat                                                                                                                                  >  2:136644/1‑251 (MQ=255)                                                                                                              cGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATc                                                                                                                               >  1:234186/1‑251 (MQ=255)                                                                                                                      gCAACGGCCCGTGGTCGGCCGCCAGCCAGAAGGAAACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTAATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAAc                                                                                                                        <  2:187211/250‑1 (MQ=255)                                                                                                                                   gTCCGTCGCAAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACt                                                                                                           <  2:219703/250‑1 (MQ=255)                                                                                                                                     ccGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGa                                                                                                         <  1:36312/250‑1 (MQ=255)                                                                                                                                                   aaGGAGACGTCTGCCCCTAGCGGGGTGTCCAGCCAGTGTCGGGCTAAGATAACCACC‑CCCTCGGCCCCGCTGTAATTCCATCCAACAACCAGGCTTCATTATAGCCTGGTTTCGGTTCATTGGCTGTGGGTTTATACCGTCCTTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTTTTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATcccc                                                                                         >  2:116149/1‑251 (MQ=255)                                                                                                                                                      gagaTTTCTGTCCCTTGCGGGTGGTCCCGCCAGTGTCGGGATAAGATAAA‑CCCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTGCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAAGTGCGGCGCTAGCTGAC‑GTCAGAATATCCCCata                                                                                      <  2:60362/251‑1 (MQ=255)                                                                                                                                                                tCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATAAAGTATTTACGGCCCGTAATTTGCCCTCGCG‑CCATGCTTACGCAGATAGGGTTTTTTAAGACGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAAC‑GACCGGCAGAATTTCCCCCTCTAACCGACct                                                                            >  2:136818/1‑251 (MQ=255)                                                                                                                                                                           gtgtCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCGGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACg                                                                 >  1:39993/1‑251 (MQ=255)                                                                                                                                                                             gtCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCCCGCTGTAATTCCATCCAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCCGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGAt                                                               >  2:21026/1‑251 (MQ=255)                                                                                                                                                                                   ccAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATACCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGtt                                                         <  2:183205/251‑1 (MQ=255)                                                                                                                                                                                     aGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTAt                                                       <  1:203141/251‑1 (MQ=255)                                                                                                                                                                                                                  cgcCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGcccat                           >  1:211341/1‑249 (MQ=255)                                                                                                                                                                                                                       cGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCaa                      >  1:24769/1‑250 (MQ=255)                                                                                                                                                                                                                             aattCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCaactgggcc               <  1:65624/248‑8 (MQ=255)                                                                                                                                                                                                                                  cATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTgag          <  1:270259/251‑3 (MQ=255)                                                                                                                                                                                                                                   aTCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTgaga         <  2:200955/251‑4 (MQ=255)                                                                                                                                                                                                                                            ccAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGAcc  >  1:238531/1‑249 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  CCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACC  >  NC_000913/65567‑66050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑