Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,176,126	C→A	E66* (GAA→TAA)	gatZ ←	putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,176,126	0	C	A	96.4%	92.5 / NA	28	E66* (GAA→TAA)	gatZ	putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (11/16);  minor base T (0/1);  total (11/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCC  >  NC_000913/2175891‑2176370                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      cGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCgg                                                                                                                                                                                                                                        >  1:51565/1‑251 (MQ=255)         aaCCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATTc                                                                                                                                                                                                                               <  1:260637/251‑1 (MQ=255)         aaCCGTTTCTGGTGCTAACGGTATGGGAGCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATTc                                                                                                                                                                                                                               <  1:7058/251‑1 (MQ=255)                         aaCGTTATGGGACCCCCCGCGCAGGAAATGGACGCATCAAGATGAATTTTACTGAAGCCGGCACGACCATATTCCTTTACCAGCTCGACGGATTTTCCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAAGTAGCGAAAGTCTGCCGGTGT‑CATTCCGTTATAACCGCCaaa                                                                                                                                                                                                               <  2:50384/251‑1 (MQ=255)                               aTGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAattgatt                                                                                                                                                                                                         >  1:30643/1‑251 (MQ=255)                                tGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAattgattg                                                                                                                                                                                                        >  1:36257/1‑251 (MQ=255)                                                                                  ccGGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATc                                                                                                                                                      <  2:80816/251‑1 (MQ=255)                                                                                     gCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCaaa                                                                                                                                                   <  1:148684/251‑1 (MQ=255)                                                                                                   cTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCtt                                                                                                                                     >  1:122745/1‑251 (MQ=255)                                                                                                       aCCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGAt                                                                                                                                 >  1:177979/1‑251 (MQ=255)                                                                                                           gCTCGACGGATTTTTCAATCGCCGCATCCGCCTTTTATTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAAATTTATCGGCAATCGTAAAAACAACTTAGCGAAAGTATGCCGGTGT‑CATTCCTGTATAACCGCCAAATTGATTAACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGGTGCGATCAAATGCCAGAGCCGCTTCGATAAc                                                                                                                              >  2:99847/1‑250 (MQ=255)                                                                                                              cggcGGATTTTTCCCTCGCCGCATCCGCTTTTTCTGGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAAGAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATTGCGGGATAACCGCCAAGTGGATGGACCTGGTTTGACGTTGCTTCAAGCAGCACGTTGCGCATGCTGTTTCTATCAAATGCCAGCGCCGCTACCATAGCCAAc                                                                                                                          <  2:153921/248‑1 (MQ=255)                                                                                                                   gATTTTTCCACCGCCGCATCCGCATTTTCTTGCTGCCAGGAGATTGGCCCCAGAGGATCGCGGCCGAGAAAAATGCGTGCGCGTGCAAACCCAGCTTTACCGGCAATGGAAAAAACAAAGTAGGGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAAGTGATTGACCTGGTTTGACGTGGCTTCAATCAGCACTGTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGTTAACCAACGTATg                                                                                                                     <  2:155102/251‑1 (MQ=255)                                                                                                                        ttCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCACCTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCa                                                                                                                 <  2:95027/250‑1 (MQ=255)                                                                                                                         tCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGACGCTTCGATAACCAACGGATGGGc                                                                                                                  >  2:229688/1‑248 (MQ=255)                                                                                                                                        gcatTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACatat                                                                                                <  1:34393/251‑1 (MQ=255)                                                                                                                                                                    ccAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCgg                                                                    <  1:260607/251‑1 (MQ=255)                                                                                                                                                                      aGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGc                                                                  >  2:171955/1‑251 (MQ=255)                                                                                                                                                                           atCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTa                                                             <  1:179866/251‑1 (MQ=255)                                                                                                                                                                              gccgccGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACg                                                          <  1:74421/251‑1 (MQ=255)                                                                                                                                                                                  ccgAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGtttt                                                      <  1:171375/251‑1 (MQ=255)                                                                                                                                                                                           atGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGttttt                                             >  2:90871/1‑251 (MQ=255)                                                                                                                                                                                            ggCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATGCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGtttttt                                            <  2:93820/250‑1 (MQ=255)                                                                                                                                                                                             gCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTGTTCATGtttttt                                            <  2:30643/250‑1 (MQ=255)                                                                                                                                                                                              cGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTcc                                          >  1:20973/1‑251 (MQ=255)                                                                                                                                                                                                  cgcgTCCAAACCCAACTTTATCGGCAATCGTAAAAACAAAGTTGCGAAAGTCTGCCGG‑GTGCAGTCCTGTAGAACCGCCAAATTGATTGACCTGGTTTGACGTTTCTTCAATCAGCACTTTACGCGTGCTGTTGCGATCAAATGCCATCGCCGCTTCGAGAACCAACGGATGGGCAGAACAGACTGAACATATGCCTAGTTGTGCACCAGCGTTATGCCGAGCAATTTACGTTTTCATGGTTTTTCCTTg                                       <  2:112715/250‑1 (MQ=255)                                                                                                                                                                                                                aCTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGc                        <  1:90871/251‑1 (MQ=255)                                                                                                                                                                                                                                       aaaTTAGCGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGcc  >  2:51707/1‑250 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      CGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGT‑CATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCC  >  NC_000913/2175891‑2176370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑