Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	45.8 / NA	14	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/5);  total (9/5)

GGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGA‑CCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGC  >  NC_000913/1652089‑1652548                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                           ggTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAATCGGGTCATTCATCTTATTAAGCCACCAATCGACGCTCTCCGGAGTCAGATGACCTTAACGAGAATGAGAAAAGAGAATATCATTGATGTTCACCTCagag                                                                                                                                                                                                                      >  2:267617/1‑249 (MQ=255)            gTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcagaac                                                                                                                                                                                                         <  1:48063/251‑1 (MQ=255)                                      gCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGttt                                                                                                                                                                               <  1:196279/251‑1 (MQ=255)                                                             aCATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAATCGGGGCATTCATCTTATTAAAGCAGCAATCGACGCTCTCCGGGGTCAAATGAAATTAAAGGGACTGAGCAAAGAACAAATAATTGTTGTTCACCTCAGAGAGTATGGCCGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAgagtaagtg                                                                                                                                                           >  1:258336/1‑240 (MQ=255)                                                                       gAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAATCGGGTCATACATCTTATTAAGCCAGAAATAGACGATCTCCGGAGGCAGAAGAAATTAACGAGACTGAGGAAAAAGAATATAATTGATGTTCACCTCAGAGAGAAAGGCAGAACAGAAAAAAAAAAATGCCCCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAAtt                                                                                                                                              >  1:266037/1‑251 (MQ=255)                                                                                         gatCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGA‑CCATTTTAcc                                                                                                                            <  1:143822/250‑1 (MQ=255)                                                                                                 gaAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTTAGCCCGCAAACGACGCTCTCCGGAGGCAGATGACATTAACGAGACTGAGGAAAGAGCATATAATTGATGTTTACCTCAGAGAGTATGGCAGAACAAAAAAACAAAAATGCACCTTTTTTTTTCAAACTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGA‑c                                                                                                                                     >  2:237787/1‑233 (MQ=255)                                                                                                 gaAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGA‑c                                                                                                                                     <  1:237787/233‑1 (MQ=255)                                                                                                       tAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAAACGGGTCATTCATCTTATTAAGCAAACCAACGACGCTCTCCGGAGGCAGATGACATTAAAGAGACTGAGCAAAAAGCATATCATTGTTGTTCACCTCCGAGAGGATGGCAGAACAGAAAAACCAAAATGCACCTTTTTTTTTTAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGAACCTTTTTAC‑GTTTACTCAATAAgg                                                                                                             >  2:177981/1‑251 (MQ=255)                                                                                                                                    acacAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGA‑CCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGc                                                                                <  1:238741/251‑1 (MQ=255)                                                                                                                                                  cAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGA‑CCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATc                                                                  >  1:81660/1‑251 (MQ=255)                                                                                                                                                                                 aTCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGA‑CCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGc                                   >  1:68887/1‑251 (MQ=255)                                                                                                                                                                                          ctcCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGA‑CCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAACATCATATCAGTCGAGACATACTTATGCCTGCTGGtt                          >  2:197687/1‑250 (MQ=255)                                                                                                                                                                                                                  agaCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGA‑CCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGc  >  1:124370/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                           GGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGA‑CCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGC  >  NC_000913/1652089‑1652548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑