Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,785	A→T	intergenic (‑5/+70)	polB ← / ← araD	DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,785	0	A	T	100.0%	91.5 / NA	29	intergenic (‑5/+70)	polB/araD	DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (14/15);  total (14/15)

GAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAA  >  NC_000913/65561‑66030                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                        gAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGGGTCCCGCCAGTGTCGGGGTAAGATAAA‑ACCCGCCTGCGCCACCCTGTAATTCCCTCCAAAAAACAGGCTTGat                                                                                                                                                                                                                               >  1:38851/1‑250 (MQ=255)       ccTTTAACGCCAGGGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTTCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGGCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAg                                                                                                                                                                                                                          <  1:166372/250‑1 (MQ=255)               gCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTc                                                                                                                                                                                                                 <  1:204193/251‑1 (MQ=255)                      gtgtCAGGCGAACGCCTTGTTCCCCCTGCAAAATATGCGGGGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTACCCTGCAACGGCCCGTTGTCCGTCCCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGAt                                                                                                                                                                                                          <  2:88722/251‑1 (MQ=255)                          cAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACATGATCGGCGGGAATAAACACCACGGACTCTTGCGGTGCAAGCGTAAACTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAAAATTCTGTCCCTTGCGGGGGGTCCCGCCAGTGTCGGGGTAAGATAAA‑ACCTGCCTGCGCCCCGCTGTAATTTCCTCAAAAAACCAAGGTTGATTTTATCCTGGTTTCGTTTGAtttgg                                                                                                                                                                                                      >  1:159591/1‑248 (MQ=255)                                  cgCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGttt                                                                                                                                                                                              <  1:34942/250‑1 (MQ=255)                                           cACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTGGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACGTCGGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGTCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTc                                                                                                                                                                                     <  2:132190/251‑1 (MQ=255)                                                               gcgcGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCGGCAACGGCCCGTGGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGcc                                                                                                                                                                 <  1:106226/251‑1 (MQ=255)                                                                cgcgGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCt                                                                                                                                                                <  1:191241/251‑1 (MQ=255)                                                                    gggAACCGGCTCGGCGGGAATAAACGCCACGGACTCTGGCGGTGCAAGCGTAACCTTCACCGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTcgc                                                                                                                                                            <  2:114530/251‑1 (MQ=255)                                                                                            cGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGttt                                                                                                                                   >  1:453/1‑251 (MQ=255)                                                                                             cccACGGACTCTTGCGGTGCAAGCGTAACCTGCACCGGCCCGTTGTCCGCCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTa                                                                                                                                  <  1:52510/250‑1 (MQ=255)                                                                                               cACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTTATTATAGCCTGGTTTCGTTTGATTGGCTGTGGGTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGGTTAtt                                                                                                                                >  2:146637/1‑250 (MQ=255)                                                                                                       ctTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCg                                                                                                                        >  1:58089/1‑251 (MQ=255)                                                                                                                cAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTACGATAAA‑AACTGCCTGCGCCACGCTGTAATTCACTCAAAAAACCCGGCTTGATTATAGCCCGGTTTCGGTTGATTGGCTGTGGTTTTATACCGTCATTAATGCCCCGAATTTGCCTTCGCGGCCAT‑CTTCCGCCAATAGTGtttctttagcagcggtttcctcc                                                                                                               >  2:166722/1‑231 (MQ=255)                                                                                                                 aaGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGGTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATTCTTACGCCGATTGTGTTTATTTAGCAGCGTTTGCTGCat                                                                                                              >  2:136644/1‑251 (MQ=255)                                                                                                                    cGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATc                                                                                                           >  1:234186/1‑251 (MQ=255)                                                                                                                            gCAACGGCCCGTGGTCGGCCGCCAGCCAGAAGGAAACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTAATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAAc                                                                                                    <  2:187211/250‑1 (MQ=255)                                                                                                                                         gTCCGTCGCAAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACt                                                                                       <  2:219703/250‑1 (MQ=255)                                                                                                                                           ccGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGa                                                                                     <  1:36312/250‑1 (MQ=255)                                                                                                                                                         aaGGAGACGTCTGCCCCTAGCGGGGTGTCCAGCCAGTGTCGGGCTAAGATAACCACC‑CCCTCGGCCCCGCTGTAATTCCATCCAACAACCAGGCTTCATTATAGCCTGGTTTCGGTTCATTGGCTGTGGGTTTATACCGTCCTTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTTTTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATcccc                                                                     >  2:116149/1‑251 (MQ=255)                                                                                                                                                            gagaTTTCTGTCCCTTGCGGGTGGTCCCGCCAGTGTCGGGATAAGATAAA‑CCCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTGCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAAGTGCGGCGCTAGCTGAC‑GTCAGAATATCCCCata                                                                  <  2:60362/251‑1 (MQ=255)                                                                                                                                                                      tCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATAAAGTATTTACGGCCCGTAATTTGCCCTCGCG‑CCATGCTTACGCAGATAGGGTTTTTTAAGACGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAAC‑GACCGGCAGAATTTCCCCCTCTAACCGACct                                                        >  2:136818/1‑251 (MQ=255)                                                                                                                                                                                 gtgtCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCGGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACg                                             >  1:39993/1‑251 (MQ=255)                                                                                                                                                                                   gtCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCCCGCTGTAATTCCATCCAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCCGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGAt                                           >  2:21026/1‑251 (MQ=255)                                                                                                                                                                                         ccAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATACCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGtt                                     <  2:183205/251‑1 (MQ=255)                                                                                                                                                                                           aGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTAt                                   <  1:203141/251‑1 (MQ=255)                                                                                                                                                                                                                        cgcCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGcccat       >  1:211341/1‑249 (MQ=255)                                                                                                                                                                                                                             cGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCaa  >  1:24769/1‑250 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                        GAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAA‑ACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCG‑CCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAC‑GGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAA  >  NC_000913/65561‑66030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑