Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,061,935	G→A	S46N (AGC→AAC)	torD →	trimethylamine‑N‑oxide reductase‑specific chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,061,935	0	G	A	90.9%	50.7 / NA	22	S46N (AGC→AAC)	torD	trimethylamine‑N‑oxide reductase‑specific chaperone
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (14/6);  minor base C (0/2);  total (14/8)
Fisher's exact test for biased strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGGAAATTGAGAAGTACAACGGAACAGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTC  >  NC_000913/1061691‑1062155                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                             ggtggAAATTGAGAAGTACAACGGAACAGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAACGAAcc                                                                                                                                                                                                                        >  1:5362/1‑251 (MQ=255)   tggAAATTGAGAAGTACAACGGAACAGTGGAGCAGGGGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCCCGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGAGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAACGAAccgc                                                                                                                                                                                                                      >  1:154312/1‑251 (MQ=255)           gagaAGTACAACGGAACAGTGGCGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAACGAACCGCCGCTCACt                                                                                                                                                                                                              >  2:83737/1‑251 (MQ=255)                   cAACGGAACAGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGt                                                                                                                                                                                                      >  1:58748/1‑251 (MQ=255)                                gAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGa                                                                                                                                                                                          >  1:18992/1‑250 (MQ=255)                                            gCGTTAAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTCCCCGCGTCGCAGGTGAAATCATGACCACGCGGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAAGGGTTTTCGTTGCTAAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGcc                                                                                                                                                                             <  1:118647/251‑1 (MQ=255)                                                       ccccGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGt                                                                                                                                                                  >  1:271061/1‑251 (MQ=255)                                                           ggggagggggtggCGCGGGGCGAATATGTCCCGGCGGCGAGGGTAAAATCAGGACCACGCTGACAGCAAAACAGATGGCCGGTGTTAACGCCGGGCTAGCGCAGTTGTTCGCCCGGGAGCGGGAGGAGGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGt                                                                                                                                                              <  2:9017/243‑1 (MQ=255)                                                           ggggAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTTCCACGCCGACAGTaagg                                                                                                                                                              >  1:215475/3‑248 (MQ=255)                                                               agaTGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTCCGTGACg                                                                                                                                                          >  2:265511/1‑251 (MQ=255)                                                                           aGTGCGGATATGTTCCCGCGTCGCTGGTGGAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGCGAACGAGCTGGAAAACCGTATTGCCACGCTGATAGTACGTGACGATGCCCGTCTgg                                                                                                                                              >  1:221655/1‑251 (MQ=255)                                                                                              gTCGCAGGTGAAATCATGACCACGCTGACAGCACAACCGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACCGTACGTGACGATGCCCGTCCGGAACTGGCCGCGGACTTTTg                                                                                                                           >  1:124408/1‑251 (MQ=255)                                                                                                        aaaTCATGACCACGCTGACAGCACAACAGATGGCCGTTGTTTACGCCTGGCTAGCGCATTTTTTCTCCGGTGATCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCGGATGGTTGAATGGGTGGGGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGGACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGttt                                                                                                                 <  1:37832/251‑1 (MQ=255)                                                                                                                 ccACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGCGCTGGAAAACCGTATTGCCACGCTGACCGTACGGGACGAAGACCGCCTGGAAACGGCCGCCGACTTTTGCGGCCCGTTTCTGATGAcc                                                                                                        >  2:104274/1‑251 (MQ=255)                                                                                                                        tacggCACAAAAAATTGCCTGTGTTTACGCCGGGCTAGCGCAGTTGTTCCCCCGTGAGCTGGACGATGAACAACTGACGCAAATCCCCAGTGCGCGGAGGGCTGAATGGTTTTCGTTGCTGAAAACCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGacaaa                                                                                                  <  2:68561/246‑1 (MQ=255)                                                                                                                           aGCACAACATATTGCTTGTGTTTAGCCCTGGCTGGCGCAGTTGTTCTCCCGTGAGCTGGACGATAAACAACTGACGAAAATCCCCAGTGCGCAGATGGCGGAAGGGTTTTCTTTGCTGAAAACCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAg                                                                                              <  2:13897/251‑1 (MQ=255)                                                                                                                                                          cTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAAc                                                                >  1:12408/1‑250 (MQ=255)                                                                                                                                                               gcAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACg                                                          >  1:16168/1‑251 (MQ=255)                                                                                                                                                                   ttgttCTCCCGTGAGCTGGACGATGAACAACTGCCGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGGAAACCGTATTGCCACGCTGGCAGTACGTGACGATGCCCGTCTTGAACTGGCCGCGGACTTTTGCCGCCTGTTTCTGATGACCGACAAACAAACGGCGCTGCCGTATGCCTCGGCCCACAAAAAGGACggacc                                                      >  2:122109/1‑247 (MQ=255)                                                                                                                                                                     gttCTCCCGTGGGCTGGAAGATGAACAACTGACGCAAATCGCCGGGGCGAAGATGGCTGAATGGTTTCCGTTGCAAAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCaa                                                     <  2:60133/250‑1 (MQ=255)                                                                                                                                                                                                              ccggTGCGCAGAGGGGTGAATGGTTTTCGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGc           <  1:30590/248‑1 (MQ=255)                                                                                                                                                                                                                       ggATGGCTGAATGGTTTTCGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTc  <  1:56273/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                             GGTGGAAATTGAGAAGTACAACGGAACAGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTC  >  NC_000913/1061691‑1062155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑