Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	882,452	T→G	T95P (ACC→CCC)	deoR ←	DNA‑binding transcriptional repressor DeoR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	882,452	0	T	G	100.0%	31.5 / NA	13	T95P (ACC→CCC)	deoR	DNA‑binding transcriptional repressor DeoR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (6/7);  total (6/7)

CCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTA  >  NC_000913/882211‑882675                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                cccgccGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTGTGAAAATTGCGTTGCTCGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAATGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGGCTGATCggg                                                                                                                                                                                                                        <  2:30006/251‑1 (MQ=255)   cgccgcAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATAGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGATTCAATAATACACGGCGTGGACGTGCCACAGTCAAAAAAGAGGGGCTGATCGGGtt                                                                                                                                                                                                                      >  1:55195/1‑251 (MQ=255)                     gATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGGCTGATCGGGTTCTACCAGCGTCGCAGcc                                                                                                                                                                                                     >  1:129347/1‑250 (MQ=255)                        atCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGGCTGATCGGGTTCTACCAGCGTCGCAGCCAGt                                                                                                                                                                                                  <  1:82057/250‑1 (MQ=255)                             ggCAAAAATTATTCAGCGATTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGGATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCCTTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGGCTGATCGGGGTCTACCAGCGTCGCAGCCAGTTTTgcc                                                                                                                                                                                            >  1:206163/1‑251 (MQ=255)                                                                     gaataTGGCGTTGCTCGCGTGAAATTCACCACCGCAAAGAAACGCGCGTCAATGGGGTGTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGTGATTTCATTATCTATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGGCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTGCTTCCACCAGGCGGTATTTTTGATCg                                                                                                                                                     <  2:36089/246‑1 (MQ=255)                                                                                tGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGTGCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTGCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTa                                                                                                                                          <  1:257713/250‑1 (MQ=255)                                                                                         gAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGGCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAAtggctggc                                                                                                                                 >  1:267353/1‑250 (MQ=255)                                                                                                                            ggTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGGCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCaa                                                                                             <  1:160043/251‑1 (MQ=255)                                                                                                                                             cAGAAAGGTATTTAGCGAATAACAACCGGCGGTAAAAGGGCATTCATTATCCATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAACAAAGAGGGGCTGATCCGGTTCTACCAGCGTCGCAGCCAGTATTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGGTTTTTGATCGCTTAACAGGGAATGGCTGGCACCACGCGGTTCCCAAACAATATAGCCGCCCAGGCAAACGCCGGGCGCACtgt                                                                            >  2:93139/1‑251 (MQ=255)                                                                                                                                                    gTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGGCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGgttgtt                                                                     <  2:2607/251‑1 (MQ=255)                                                                                                                                                                                                            aatCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGGCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTa              <  2:7475/250‑1 (MQ=255)                                                                                                                                                                                                                       tggtggtgTCAAAGTCAACAAAAAGGGGCTGATCGGGTTCTACCAGCGTCGCAGCAAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTCACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGGCTTTAAGATGTAACttt  >  2:27730/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                CCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTA  >  NC_000913/882211‑882675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑