Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,454	A→G	R109R (CGT→CGC)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,454	0	A	G	100.0%	62.6 / NA	21	R109R (CGT→CGC)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/11);  total (10/11)

GGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTATAGAAACCCACTTGAGCGGCGGACGATA‑GTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAA  >  NC_000913/65214‑65687                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                          ggaggCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCAATATCAAAAGAAACCCACTTGAGCGGCGGACGAAAAGT‑GGGATGCGGTTTTAGACGGGCATTAAAGAAAGCGGCCTTGGGCATATCACCCTCGACCCCCACCGGTGGGGTGATAAACCGCTCCCTCAGATAGCGTTCTGGCGGGCGCACAttgg                                                                                                                                                                                                                                 >  2:124863/1‑248 (MQ=255)    ggCGTCGCCATTCTCTGGCCCCAGCATGTAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTGCAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATA‑GTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCGTTGTGCGTATCACCCTCGACCCACACCGGTGAGGTGATAAGCCGCTCCGTCAGATAGCGTTCTGGCGGGCGCACGTCGGCCt                                                                                                                                                                                                                              <  2:106304/251‑1 (MQ=255)                                        gcgcTGCCCGCAGCCGTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATA‑GTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCttt                                                                                                                                                                                           <  1:208998/250‑1 (MQ=255)                                         cgcTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATA‑GTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTc                                                                                                                                                                                         <  1:53938/251‑1 (MQ=255)                                                                                                        ataGAAACACACTTGAGCGGCGGACGATA‑GTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGCAAATc                                                                                                                          >  1:218773/1‑251 (MQ=255)                                                                                                                      gAGCGGCGGACGATA‑GTCGGGATGCGGGTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCGCCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGc                                                                                                             <  2:82465/250‑1 (MQ=255)                                                                                                                               aCGATA‑GTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGc                                                                                                   >  1:200955/1‑251 (MQ=255)                                                                                                                                            aTGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGtt                                                                                       <  2:270358/251‑1 (MQ=255)                                                                                                                                                         cGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAat                                                                          <  1:151712/251‑1 (MQ=255)                                                                                                                                                                tAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGACTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGGGAAAATCCTTTAAAGCCAGCGGTGTCAGGCGAAAGCCTTGGTCACCCTGCCAAATATGCTg                                                                    >  2:36312/1‑250 (MQ=255)                                                                                                                                                                       agcgCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGAGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCTCATCGGCCTCGTAGACGGTAACGCCACCGTCACGCGGGCGCTTTTCGTAATTCATTAATTGTCGATGGGCGCGGCAGTAAAGGCCATACACCGGCTGGCGGGGAAAATCCTTTAACCCCAGAGGGTTCCGGCGAACGCCCTGTTAATCCTGTCAAATAATCTGAGcgcgtg                                                            >  1:136158/4‑249 (MQ=255)                                                                                                                                                                                gtgCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGa                                                    <  1:78878/250‑1 (MQ=255)                                                                                                                                                                                        cACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCgggg                                           >  1:183205/1‑250 (MQ=255)                                                                                                                                                                                             tCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGGATaa                                       >  1:179638/1‑250 (MQ=255)                                                                                                                                                                                                       ccGGTGCGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGCAGGGAACGCCACATTCACGAAGGCGCTTTTCGTAATTCATCCATTGGCGATGGGCGCGACAGGAAAAGCCATACAACGGATGGCGGTGAAAATCCTTTTACGCAAGCGGGGTCAGGCGGAAGGCTTGTTTACCCCGGAAAAAATGCTGAGCGCGGGGAAACCGAGCGGCgggggtaaaacgccccgg                             >  2:53194/1‑235 (MQ=255)                                                                                                                                                                                                            gaggtgaTAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTg                       <  2:218773/251‑1 (MQ=255)                                                                                                                                                                                                              ggtgaTAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCg                     <  1:4000/251‑1 (MQ=255)                                                                                                                                                                                                                    aaaCCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAAAGCCAccggatctttgggtgca                >  1:112806/1‑234 (MQ=255)                                                                                                                                                                                                                              aTCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGGCTAAAACCCACGGACTCTTGCGGGGCAAGCGTAAccat     >  1:261408/1‑249 (MQ=255)                                                                                                                                                                                                                                cAGATAGCGTTCTGGCGGGCGCGCATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGc    <  2:84616/250‑1 (MQ=255)                                                                                                                                                                                                                                 aGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCaa  <  2:101386/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                          GGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTATAGAAACCCACTTGAGCGGCGGACGATA‑GTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAA  >  NC_000913/65214‑65687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑