Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,381	T→C	T134A (ACT→GCT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,381	0	T	C	100.0%	54.9 / NA	20	T134A (ACT→GCT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/10);  total (10/10)

GTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTATAGAAACCCACTTGAGCGGCGGACGATA‑GTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTG‑CATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTG  >  NC_000913/65139‑65621                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  gTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGGCGGTA‑GGCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATtgt                                                                                                                                                                                                                                           >  1:187211/1‑251 (MQ=255) gTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATA‑GTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATtgt                                                                                                                                                                                                                                           <  1:181734/251‑1 (MQ=255)   tGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATA‑GTCGGGATGCGGTTTCAGCCGGGCATTAAAGATAGCGCCATTGTG‑c                                                                                                                                                                                                                                        >  1:270358/1‑251 (MQ=255)            aGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCACTATCCATAGAAAACCACTTGAGCGGCGGGCGGTA‑GTCGGGATGCGGTTTCATCAGGGCATTAACGAGAGCGCCATTTTG‑CCTATCAccc                                                                                                                                                                                                                               >  2:34942/1‑251 (MQ=255)                          tccagtagcTGCGGGCGGCTGGCGACGTATTCCAGTGCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATA‑GTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTG‑CATATCACCCTCGACCCACACCg                                                                                                                                                                                                                  <  1:239723/242‑1 (MQ=255)                            cagtagcTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATA‑GTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTG‑CATATCACCCTCGACCCACACCggtg                                                                                                                                                                                                               <  1:88148/245‑1 (MQ=255)                                agcTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTTAATATCAATAGAAAACCACTTTAGCGGCGGACGATA‑GTCGGGGTGGGGGTTCCGACGGGGCTTAACGATAGCGCCCTT‑TGTCATATAACCCTCGACCCCCACCggggaggg                                                                                                                                                                                                           >  2:52929/3‑250 (MQ=255)                                 gcTGCGGGCGGCTGGCGACGTATTCAAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATA‑GTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTG‑CATATCACCCTCGACCCACACCggtgagggg                                                                                                                                                                                                          >  1:58604/2‑249 (MQ=255)                                                            gTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATA‑GTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTG‑CATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCt                                                                                                                                                                               <  2:41316/251‑1 (MQ=255)                                                                    tcgaGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGAAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATA‑GCCGGGATGCGGTTTCAGACGGGCATTAACGGTAGCGACATTGTG‑CATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCgggcg                                                                                                                                                                       >  1:10328/4‑248 (MQ=255)                                                                            ggaggCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCAATATCAAAAGAAACCCACTTGAGCGGCGGACGAAAAGT‑GGGATGCGGTTTTAGACGGGCATTAAAGAAAGCGGCCTTGGG‑CATATCACCCTCGACCCCCACCGGTGGGGTGATAAACCGCTCCCTCAGATAGCGTTCTGGCGGGCGCACAttgg                                                                                                                                                               >  2:124863/1‑248 (MQ=255)                                                                               ggCGTCGCCATTCTCTGGCCCCAGCATGTAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTGCAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATA‑GTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCGTTGTG‑CGTATCACCCTCGACCCACACCGGTGAGGTGATAAGCCGCTCCGTCAGATAGCGTTCTGGCGGGCGCACGTCGGCCt                                                                                                                                                            <  2:106304/251‑1 (MQ=255)                                                                                                                   gcgcTGCCCGCAGCCGTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATA‑GTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTG‑CATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCttt                                                                                                                         <  1:208998/250‑1 (MQ=255)                                                                                                                    cgcTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATA‑GTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTG‑CATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTc                                                                                                                       <  1:53938/251‑1 (MQ=255)                                                                                                                                                                                   ataGAAACACACTTGAGCGGCGGACGATA‑GTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTG‑CATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGCAAATc                                                        >  1:218773/1‑251 (MQ=255)                                                                                                                                                                                                 gAGCGGCGGACGATA‑GTCGGGATGCGGGTTCAGACGGGCATTAACGATAGCGCCATTGTG‑CATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCGCCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGc                                           <  2:82465/250‑1 (MQ=255)                                                                                                                                                                                                          aCGATA‑GTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTG‑CATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGc                                 >  1:200955/1‑251 (MQ=255)                                                                                                                                                                                                                       aTGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTG‑CATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGtt                     <  2:270358/251‑1 (MQ=255)                                                                                                                                                                                                                                    cGGGCATTAACGATAGCGCCATTGTG‑CATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAat        <  1:151712/251‑1 (MQ=255)                                                                                                                                                                                                                                           tAACGATAGCGCCATTGTG‑CATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGACTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGGGAAAATCCTTTAAAGCCAGCGGTGTCAGGCGAAAGCCTTGGTCACCCTGCCAAATATGCTg  >  2:36312/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  GTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTATAGAAACCCACTTGAGCGGCGGACGATA‑GTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTG‑CATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTG  >  NC_000913/65139‑65621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑