Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	92,327	G→T	P305P (CCG→CCT)	ftsI →	peptidoglycan DD‑transpeptidase FtsI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	92,327	0	G	T	100.0%	88.7 / NA	28	P305P (CCG→CCT)	ftsI	peptidoglycan DD‑transpeptidase FtsI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/13);  total (15/13)

CTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACT  >  NC_000913/92080‑92566                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                ctACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGCACCTGGc                                                                                                                                                                                                                                              >  2:251555/1‑251 (MQ=255)     ggACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGATCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCaa                                                                                                                                                                                                                                          <  2:255919/250‑1 (MQ=255)        cAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACgg                                                                                                                                                                                                                                       >  2:190041/1‑251 (MQ=255)               gctgcgcACAACCTGGCGCTGAGTATTGATGAACGCCTGGAGGCGCTGGTGTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCAGACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAAcc                                                                                                                                                                                                                                <  2:30199/248‑1 (MQ=255)                             ggCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGtgatga                                                                                                                                                                                                                  <  1:13696/251‑1 (MQ=255)                              gcgcTGAGTATTGATGAACGCCTTCAGACGATGGTTTATCGCGAAATGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGAGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCTGACATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTCTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGAc                                                                                                                                                                                                                 >  1:268821/1‑251 (MQ=255)                                            tgaACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCATGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAAcgtg                                                                                                                                                                                                   >  2:131918/1‑251 (MQ=255)                                                     gCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGAAACGTGGCgtggtgg                                                                                                                                                                                          >  2:79052/1‑250 (MQ=255)                                                            caggtgtATCGCGAACTGAACAATGCGGTGGCCTTTAACAATGGTGAATCTGGTAGCGACGAGCTGGTGGATGTAAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATACGTAACCGTACCATCACCGAAGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCACCGTGGCGTGGTGCGTGaaa                                                                                                                                                                                    >  2:205831/7‑250 (MQ=255)                                                                                  atgcgGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCt                                                                                                                                                             <  1:199881/249‑1 (MQ=255)                                                                                                 aCAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAGCAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGGACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGc                                                                                                                                              <  1:264066/251‑1 (MQ=255)                                                                                                               ggTAGCGCCGTGCTGGTGGATGTCATCACCGGTGACGTGCTGGCGATGGCTAACAGCCCGTCATCCAACCCTAACAATCTGAGCGGCACGCCGAAAGCGGCGATGCGCAACCGTACCATCACCGACGAGTTTGAACCTGGCTCAACGGTTAAACCGCTGGCGGTGATGACCGCGTTTCAACGTGGCGAGGTGCGGGAAAACTCGGGACTCAATACCCTTCCTTATAGAATTAACGGCCACGTAATCaa                                                                                                                                   >  2:263794/1‑248 (MQ=255)                                                                                                                     gcCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGc                                                                                                                          >  1:86759/1‑251 (MQ=255)                                                                                                                            tggtggATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTAc                                                                                                                   >  2:189421/1‑251 (MQ=255)                                                                                                                                 gATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGa                                                                                                              <  1:189421/251‑1 (MQ=255)                                                                                                                                   tGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAAt                                                                                                            >  2:4131/1‑251 (MQ=255)                                                                                                                                                   gctcTGGCGATGGCTGACAGCCCGTCATACAACCCGAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCAGCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCTTTGCTACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCAGACCTTATCGAATGAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGt                                                                                            <  1:152867/248‑1 (MQ=255)                                                                                                                                                                                        aCAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTg                                                       >  2:68687/1‑251 (MQ=255)                                                                                                                                                                                         cAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTgg                                                      <  1:247422/251‑1 (MQ=255)                                                                                                                                                                                                    gCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCCGGCGTTAGCGATg                                           >  1:58517/1‑251 (MQ=255)                                                                                                                                                                                                        gCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGCTGGAGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACTCTACAGCGAATTAACCCTGCCCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGt                                       >  2:32968/1‑251 (MQ=255)                                                                                                                                                                                                              agagGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAg                                 <  1:79052/251‑1 (MQ=255)                                                                                                                                                                                                                 ggCGATGCGTAACCGTACCAGCATCGACTTGGTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATGCCGTATCGTATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCTGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGt                              <  1:26233/251‑1 (MQ=255)                                                                                                                                                                                                                                   cATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCAc            >  2:185383/1‑251 (MQ=255)                                                                                                                                                                                                                                    aTCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGTAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACg           >  2:182274/1‑251 (MQ=255)                                                                                                                                                                                                                                    aTCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACg           <  1:218168/251‑1 (MQ=255)                                                                                                                                                                                                                                        ccGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGtttt       <  2:43444/251‑1 (MQ=255)                                                                                                                                                                                                                                             gtgtTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACt  <  2:121278/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                CTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACT  >  NC_000913/92080‑92566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑