Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	89,699	T→C	Y22Y (TAT→TAC)	mraZ →	DNA‑binding transcriptional repressor MraZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	89,699	0	T	C	100.0%	65.3 / NA	22	Y22Y (TAT→TAC)	mraZ	DNA‑binding transcriptional repressor MraZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/9);  total (13/9)

GTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTATCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAATC  >  NC_000913/89477‑89915                                                                                                                                                                                                                               |                                                                                                                                                                                                                         gTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCg                                                                                                                                                                                              >  1:186496/1‑251 (MQ=255)    ggACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTGCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCAGGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCTGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGt                                                                                                                                                                                            <  2:10079/250‑1 (MQ=255)        gTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGTTCAAAt                                                                                                                                                                                       <  1:219046/251‑1 (MQ=255)         tAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCCCGGTAAACTCCTTTCAGTTGGAAATTGTGGGGAAAAGTGGGAATAAGTGGTTAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGTGCGCTTATCAGTGCCTACCCGTTACCGGGCACCGCTGCTTGAGAACGCTGCAGGTccactg                                                                                                                                                                                      >  2:45856/1‑246 (MQ=255)                      gCTGATATTCGCCGTAAACATCGGGTTTATTACCTCGGTCTGCCTTGTGACTGGCTTGACAAGCTTTTACTCAGCCCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGCGGCTGGAATGTTCCGGGGAGCAACGTTAGTCAATCTCGCCAGCAAAGGGCGCTTATCCGTGCCTACCCGTTCCCGTGAACCGCTGCGTGAGAACGCTGCCGGTCAAATGGTTCGCACCAttt                                                                                                                                                                         >  2:227655/1‑250 (MQ=255)                        tGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCAGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTCTCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGAc                                                                                                                                                                       >  1:128770/1‑251 (MQ=255)                                    tAAACATCGGGTTTTTTACCTCGGTATGCCTTGCGACTGGCTTGAAAAGCTTTTCCTCCCCTCCGTAAACTCCTTTAAGTGGGTAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGGCAAACTCGACATCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTCATTGAGAACGATGCCCGTCAAATGGTTTGCACCATTGACATTCATCAcacg                                                                                                                                                           >  2:89596/1‑248 (MQ=255)                                          tCGGGTTTTTTACCTCGGTATGCTTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGTGAAGGGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCATCGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGATAGCTCTGTCTGTCAAATGGTTTGCACCATTGACATTCATCACCCGTGCct                                                                                                                                                      <  1:116527/250‑1 (MQ=255)                                            gggTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTCATCACCCGTGCctgct                                                                                                                                                   <  2:75625/251‑1 (MQ=255)                                                                    gtgACAGGCTTGAAAAGTTTTTCATCCGCTCCGTAAAATCCTTTCAGAGGGAAATTGTGGGGCAAAGTGGGCATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAAAGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACCGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTCATCACCCGTGCCCGCTCCTTTACCCCCTACCTGCATgcg                                                                                                                            >  2:261918/1‑248 (MQ=255)                                                                        cTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTCATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAAtt                                                                                                                       >  1:186251/1‑251 (MQ=255)                                                                         tGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTCATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAAtt                                                                                                                       >  1:252392/1‑250 (MQ=255)                                                                           gCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGACATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTCATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATc                                                                                                                    >  1:122865/1‑251 (MQ=255)                                                                                                   cGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTCATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCGCGTCTGTCg                                                                                            <  1:28192/251‑1 (MQ=255)                                                                                                       aaCTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGTGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTCATCACCCGTGCCTGCTGCTTTCCCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCTCGTCTGTCGAGCa                                                                                        <  2:173967/251‑1 (MQ=255)                                                                                                                                                                      gggAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTCATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgatgg                         <  2:47985/251‑1 (MQ=255)                                                                                                                                                                                  aGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGACAACGCTGCCGGTCAAATGGTTTGCACCAGTGACATGCATCACCCGGGCCTGCTGCGTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCGCGTCTGTCTAGCGTGAACCCGGTTGAGCTCCGTGTGCAGCGCCTACTGGTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTc             <  1:175603/251‑1 (MQ=255)                                                                                                                                                                                    tcaatatagACAGCAAAGGGCGCTTATCAGTGCCTACACGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTCATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCg            >  2:159759/9‑250 (MQ=255)                                                                                                                                                                                     cAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTCATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGAt          >  1:125190/1‑251 (MQ=255)                                                                                                                                                                                      aaTCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTCATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGAtt         >  2:119577/1‑251 (MQ=255)                                                                                                                                                                                            gACAGCAAAGGGCGCTTATCAGTGCCTACCCGATACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTCATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAAt   >  1:262550/1‑251 (MQ=255)                                                                                                                                                                                             aCAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTCATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAATc  <  1:51746/251‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                         GTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTATCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAATC  >  NC_000913/89477‑89915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑