Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 86,315 C→T T229M (ACG→ATG)  ilvI → acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091386,3150CT96.2% 66.6 / ‑3.7 26T229M (ACG→ATG) ilvIacetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (12/13);  total (12/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.00e-01

AGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCC  >  NC_000913/86080‑86556
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                 
aGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGaa                                                                                                                                                                                                                                    <  1:103820/251‑1 (MQ=255)
         ccAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAAGCTGCCCgt                                                                                                                                                                                                                           <  1:232355/251‑1 (MQ=255)
                    agtCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCAt                                                                                                                                                                                                                <  2:83625/251‑1 (MQ=255)
                      tCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTg                                                                                                                                                                                                              >  1:237490/1‑251 (MQ=255)
                        gTTGATTTACCGAAAGATATTATTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCCTCAGCAGTTGAAAGAAATGGTGGAGGGGTTGGATCTGACCGTTGTTTtctcctttat                                                                                                                                                                                                            >  2:109548/1‑245 (MQ=255)
                          tGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATg                                                                                                                                                                                                           <  1:182174/250‑1 (MQ=255)
                                                aaTCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAAc                                                                                                                                                                                    >  1:145931/1‑251 (MQ=255)
                                                   ccGGCGCACAAATTCCCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCCTCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCTTCATTGATGGGGCTGGGGGCGTTTGCGGCACCGcc                                                                                                                                                                                 >  2:244854/1‑250 (MQ=255)
                                                            aaaatACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTGGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGGTGTTTCCTCATTGATgg                                                                                                                                                                                                          <  2:177594/213‑1 (MQ=255)
                                                            aaaTTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAACCGCTGGTGGCGGCAAAACAACCGGTTGTCTACGTAGGTGGTGGGGCAATAACGGCGGGCTGCCATCAGCAGTTGAAAGAACTGGGGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATgg                                                                                                                                                                                                          >  1:177594/1‑217 (MQ=255)
                                                                   ccTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGc                                                                                                                                                                 >  1:92309/1‑251 (MQ=255)
                                                                                     cGGTCAGTATGCGTTCTTACAATCCCACTACTACCGTACATAAAGGGCAAATTAATCGTGCTCTGCAAACGCTGGTGACGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGTCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGATGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGTGGCTGGGGTCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACgt                                                                                                                                                <  1:245978/250‑2 (MQ=255)
                                                                                     cGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCTTTGAATCTGCCCGTTGTTTCCTCATTGATGGTGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACgg                                                                                                                                                <  1:245976/250‑1 (MQ=255)
                                                                                         cAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCt                                                                                                                                           <  2:18333/251‑1 (MQ=255)
                                                                                         cAGTATGCGTTCTTACAATCCCACTACGACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTAcc                                                                                                                                            <  2:237490/250‑1 (MQ=255)
                                                                                               gCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAg                                                                                                                                     <  1:269064/251‑1 (MQ=255)
                                                                                                                                                     gCAAACGCTGGGGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTCGAATCTGCCCGTTGTGTCCTCATTGATGGGGCTGGGGGCGTTCCCGGCAACGCAGCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGGTTtg                                                                               <  2:191777/251‑1 (MQ=255)
                                                                                                                                                        aaCGCTGGTGGCGGCAAAAACACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCCCTGGGCATGGTGGGGATGCACGGTAACTACGCAGCCAATATGACGATGCATAACGCGGATGTGATTTTCTCCGCCggggggcgagttgttg                                                                            >  2:156629/1‑239 (MQ=255)
                                                                                                                                                                 ggCGGAAAAAAAACCGGTTGTCTACGTAGGTGGCGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGGGGAGGCGTTGAATCTGCCCGGTGTTTCCTCATTGATGGGGCAGGGGGCGTTTCCGGCAAAGCCTCGTCAGGAACTGGGCCTGCTGGGAATGCCCGGTACCTACGAAGCCCAAATGACGATGCATAACGCGGATGTTATTTTCTCCGTCGGGGTACGATTTGATTACCGAacgc                                                                   >  2:44587/2‑250 (MQ=255)
                                                                                                                                                                       aaaaaaaCCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCCTTGATGGGGCTGGGGGCGGTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCCCGGTAACTACGCAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGGCGGGGGACGATTTGATGACCGAACGCCGAAcc                                                             >  2:141046/1‑250 (MQ=255)
                                                                                                                                                                                            aggtggTGGGGCAATCACGGCGGGCTGCCATCAGCCGGTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGCTGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAAAAATCTGGCAAAGTACTGCCCa                                         >  1:236916/5‑250 (MQ=255)
                                                                                                                                                                                                    gggCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCaat                                <  2:5321/251‑3 (MQ=255)
                                                                                                                                                                                                                cggGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCCTATc                    >  2:241610/1‑251 (MQ=255)
                                                                                                                                                                                                                       ccATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGCTGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGCCGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTg             >  2:161919/1‑251 (MQ=255)
                                                                                                                                                                                                                           cagcagTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATcc         <  1:48614/251‑1 (MQ=255)
                                                                                                                                                                                                                                   gaaagaaaCGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCGCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTcc  <  1:226907/250‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                 
AGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCC  >  NC_000913/86080‑86556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: