Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	86,271	C→T	G214G (GGC→GGT)	ilvI →	acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	86,271	0	C	T	100.0%	76.6 / NA	27	G214G (GGC→GGT)	ilvI	acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/14);  total (13/14)

AAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACA  >  NC_000913/86026‑86497                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                   aaGCAACCGGAAGACATTCCGCAGATGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGACACCCAGGACCAGTAGTCGTTGATTTACCGCAAGATATTCTTAATCAGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACAGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGtggtgg                                                                                                                                                                                                                               >  2:238532/1‑251 (MQ=255)        cGGAAGACATTCCGCAGGTGCTGCAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCCGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAACACCGGTTGTCTCCGTAGGTGGTGGGGCAATc                                                                                                                                                                                                                        >  2:269064/1‑251 (MQ=255)          gAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGGCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTTCCCTATGTCTGGCCGGAGTCGGTCAGTAGGCGTTCTTACAAGCGCACTACTACCGGACATAAATGGCAAATGAAGCGTGCTCTTCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCAc                                                                                                                                                                                                                      <  1:165024/251‑1 (MQ=255)                     gCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCTGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGcctcc                                                                                                                                                                                                           >  2:182174/1‑247 (MQ=255)                               aaaaaGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGtt                                                                                                                                                                                                 <  1:114415/251‑1 (MQ=255)                               aaaaaGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGGGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTCCCAACAGCAGtt                                                                                                                                                                                                 >  2:10444/1‑251 (MQ=255)                                        ttCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTgaaagaaa                                                                                                                                                                                         <  1:243046/250‑1 (MQ=255)                                              ctggcGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCCAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGGCATCACCAGTTgtgagaaaagggggg                                                                                                                                                                                  >  2:83205/1‑237 (MQ=255)                                                       aGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGaa                                                                                                                                                                         <  1:103820/251‑1 (MQ=255)                                                                ccAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAAGCTGCCCgt                                                                                                                                                                <  1:232355/251‑1 (MQ=255)                                                                           agtCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCAt                                                                                                                                                     <  2:83625/251‑1 (MQ=255)                                                                             tCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTg                                                                                                                                                   >  1:237490/1‑251 (MQ=255)                                                                               gTTGATTTACCGAAAGATATTATTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCCTCAGCAGTTGAAAGAAATGGTGGAGGGGTTGGATCTGACCGTTGTTTtctcctttat                                                                                                                                                 >  2:109548/1‑245 (MQ=255)                                                                                 tGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATg                                                                                                                                                <  1:182174/250‑1 (MQ=255)                                                                                                       aaTCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAAc                                                                                                                         >  1:145931/1‑251 (MQ=255)                                                                                                          ccGGCGCACAAATTCCCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCCTCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCTTCATTGATGGGGCTGGGGGCGTTTGCGGCACCGcc                                                                                                                      >  2:244854/1‑250 (MQ=255)                                                                                                                   aaaatACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTGGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGGTGTTTCCTCATTGATgg                                                                                                                                               <  2:177594/213‑1 (MQ=255)                                                                                                                   aaaTTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAACCGCTGGTGGCGGCAAAACAACCGGTTGTCTACGTAGGTGGTGGGGCAATAACGGCGGGCTGCCATCAGCAGTTGAAAGAACTGGGGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATgg                                                                                                                                               >  1:177594/1‑217 (MQ=255)                                                                                                                          ccTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGc                                                                                                      >  1:92309/1‑251 (MQ=255)                                                                                                                                            cGGTCAGTATGCGTTCTTACAATCCCACTACTACCGTACATAAAGGGCAAATTAATCGTGCTCTGCAAACGCTGGTGACGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGTCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGATGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGTGGCTGGGGTCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACgt                                                                                     <  1:245978/250‑2 (MQ=255)                                                                                                                                            cGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCTTTGAATCTGCCCGTTGTTTCCTCATTGATGGTGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACgg                                                                                     <  1:245976/250‑1 (MQ=255)                                                                                                                                                cAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCt                                                                                <  2:18333/251‑1 (MQ=255)                                                                                                                                                cAGTATGCGTTCTTACAATCCCACTACGACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTAcc                                                                                 <  2:237490/250‑1 (MQ=255)                                                                                                                                                      gCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAg                                                                          <  1:269064/251‑1 (MQ=255)                                                                                                                                                                                                            gCAAACGCTGGGGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTCGAATCTGCCCGTTGTGTCCTCATTGATGGGGCTGGGGGCGTTCCCGGCAACGCAGCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGGTTtg                    <  2:191777/251‑1 (MQ=255)                                                                                                                                                                                                               aaCGCTGGTGGCGGCAAAAACACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCCCTGGGCATGGTGGGGATGCACGGTAACTACGCAGCCAATATGACGATGCATAACGCGGATGTGATTTTCTCCGCCggggggcgagttgttg                 >  2:156629/1‑239 (MQ=255)                                                                                                                                                                                                                        ggCGGAAAAAAAACCGGTTGTCTACGTAGGTGGCGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGGGGAGGCGTTGAATCTGCCCGGTGTTTCCTCATTGATGGGGCAGGGGGCGTTTCCGGCAAAGCCTCGTCAGGAACTGGGCCTGCTGGGAATGCCCGGTACCTACGAAGCCCAAATGACGATGCATAACGCGGATGTTATTTTCTCCGTCGGGGTACGATTTGATTACCGAacgc        >  2:44587/2‑250 (MQ=255)                                                                                                                                                                                                                              aaaaaaaCCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCCTTGATGGGGCTGGGGGCGGTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCCCGGTAACTACGCAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGGCGGGGGACGATTTGATGACCGAACGCCGAAcc  >  2:141046/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                   AAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACA  >  NC_000913/86026‑86497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑