Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	83,371	G→A	G53G (GGC→GGT)	leuA ←	2‑isopropylmalate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	83,371	0	G	A	100.0%	96.3 / NA	29	G53G (GGC→GGT)	leuA	2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/12);  total (17/12)

GTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGG‑AAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACT  >  NC_000913/83134‑83611                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  gtAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAagaa                                                                                                                                                                                                                                       <  1:96336/250‑2 (MQ=255)          ggCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTAATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATAGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAAc                                                                                                                                                                                                                            >  1:115023/1‑251 (MQ=255)             gcgTTTCCCCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCAGACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGa                                                                                                                                                                                                                         <  2:128768/251‑1 (MQ=255)                tttCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGACtt                                                                                                                                                                                                                      >  1:81209/1‑251 (MQ=255)                   cACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGACTTcc                                                                                                                                                                                                                    >  1:95654/1‑250 (MQ=255)                      cATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGACTTCCATc                                                                                                                                                                                                                 >  1:217235/1‑250 (MQ=255)                               agCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGACTTCCATCACGTCAacac                                                                                                                                                                                                       >  1:186197/1‑251 (MQ=255)                                   cgTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAGGGTATGAATACGGAAGGCGTCGGCAACTTTCAAGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCATCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTGGCAATGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGACTTCCATCACGTCAACACCCAt                                                                                                                                                                                                   >  1:172/1‑251 (MQ=255)                                       cGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGACTTCCATCACGTCAACACCCATACGc                                                                                                                                                                                               >  2:230845/1‑251 (MQ=255)                                                              cgcAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGc                                                                                                                                                                          >  1:128072/1‑249 (MQ=255)                                                                          gCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAATGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTTATTCAAAATCACCCGGCGAAGAAACGTGG‑AAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTGTTTCTTTcac                                                                                                                                                            <  1:54268/251‑1 (MQ=255)                                                                                        cGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCt                                                                                                                                              >  1:67116/1‑251 (MQ=255)                                                                                         gAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTg                                                                                                                                             >  2:20637/1‑251 (MQ=255)                                                                                                  aTAAAGGTATGAATACGTAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCACTTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTg                                                                                                                                    >  2:144427/1‑251 (MQ=255)                                                                                                                        cTTCGGCGACTCTCAGGGATTCGGCCGCCACGTCGCTATCTTTCTCCACGCAGCGCGATAAAGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCCCACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGt                                                                                                               >  2:267642/1‑250 (MQ=255)                                                                                                                          tCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATTCGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTa                                                                                                              <  1:81067/249‑1 (MQ=255)                                                                                                                                        ggATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACtt                                                                                              <  1:69436/251‑1 (MQ=255)                                                                                                                                         gATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTg                                                                                             <  2:52687/251‑1 (MQ=255)                                                                                                                                                       gTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGGAAACCGACTTCCATCACGTCACCACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGtt                                                                                >  1:152111/1‑251 (MQ=255)                                                                                                                                                                           aGCGAGCTCACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGCTTCAAAATCACCCGGCGACGAAACGGGG‑AAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCtttt                                                              >  1:51953/1‑248 (MQ=255)                                                                                                                                                                                   aaCGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTc                                                    <  1:52071/250‑1 (MQ=255)                                                                                                                                                                                         caTACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGTG‑AAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATTGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCgg                                             <  1:164240/251‑1 (MQ=255)                                                                                                                                                                                              gcgGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGt                                                                                                                                                                       >  1:165728/1‑124 (MQ=255)                                                                                                                                                                                              gcgGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGt                                                                                                                                                                       <  2:165728/124‑1 (MQ=255)                                                                                                                                                                                                ggCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGGGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATaaa                                      <  2:128072/251‑1 (MQ=255)                                                                                                                                                                                                               ggcgagcgATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGACTTCCATCACATCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGATTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCTCAATg                       <  1:150056/251‑1 (MQ=255)                                                                                                                                                                                                                 cgagcgATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGtt                                                                                >  2:141468/4‑192 (MQ=255)                                                                                                                                                                                                                 cgagcgATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGG‑AAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGtt                                                                                <  1:141468/189‑1 (MQ=255)                                                                                                                                                                                                                                    aTTCAAAATCACCCGGCGAAGACACGGGG‑AAACCGACTTCCATCACGTCAACACCCATACGCACAAGGGCAAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGATTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCACCTCGCTTCGGGCATCCAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACt  >  2:195400/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  GTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGG‑AAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACT  >  NC_000913/83134‑83611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑