Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	82,933	G→A	I199I (ATC→ATT)	leuA ←	2‑isopropylmalate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	82,933	0	G	A	100.0%	51.2 / NA	17	I199I (ATC→ATT)	leuA	2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/10);  total (7/10)

GGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCG  >  NC_000913/82707‑83169                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             ggCTAACTAACTGGCGGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTGTGTCGATGTTa                                                                                                                                                                                                                         <  1:161322/248‑1 (MQ=255)         aactGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGtt                                                                                                                                                                                                               <  1:36608/250‑1 (MQ=255)                               ccTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCGGATGATTc                                                                                                                                                                                          <  1:62466/249‑1 (MQ=255)                                                    ggACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGgt                                                                                                                                                                    <  1:195327/250‑1 (MQ=255)                                                                        cGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAAt                                                                                                                                               >  2:119428/1‑251 (MQ=255)                                                                         gAACTTTGATCCGCATACTGACTTCTTCAAGGGCACAGTATACGGCACGCTCGCCGATCCCCTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGCCCGCCAGGCCTCAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTCGGCACGCGTTCATACAGGCCCTTGATGATTTCGGCGAACTCAAACGGCATGGTGTAGCCAACGGTGTTCGGAATg                                                                                                                                              >  2:157233/1‑251 (MQ=255)                                                                                                         gAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGCTGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGGGGTGGCACCGGCATTAATcgccgc                                                                                                              >  1:182371/1‑251 (MQ=255)                                                                                                                                   cccGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCAGTAATCGCGGCTTCGACCACGCGCGCCAGATCGGCaa                                                                                    <  1:86418/251‑1 (MQ=255)                                                                                                                                                    ccACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCTCCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGc                                                                    <  1:156407/250‑1 (MQ=255)                                                                                                                                                    ccACCTGGCGTGCAACGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGc                                                                    >  1:52687/1‑250 (MQ=255)                                                                                                                                                                 aCCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGGGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGaa                                                       >  2:52071/1‑250 (MQ=255)                                                                                                                                                                                              cGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATGCCGGCGAACTCAAACGGCATGGTTTAGCCCGCTGTGTCCGGAATGTTGATGGTGGTGGCCCCGGCATTAATCGCCGCTTCGACCTCTCGCGCCAGATCGGCAATGGGTGTGGGCGCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGgcg                         <  1:174201/251‑1 (MQ=255)                                                                                                                                                                                                  cGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCAc                                                                                                                                                           <  2:115209/117‑1 (MQ=255)                                                                                                                                                                                                  cGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCAc                                                                                                                                                           >  1:115209/1‑117 (MQ=255)                                                                                                                                                                                                         cctaaaTCGTCGGGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCTGTGTAATTACGGGCGCGTTTCACCat              <  2:250100/248‑1 (MQ=255)                                                                                                                                                                                                                    gtgagTATGGACGGAAATAATGGCTTTGGCGATGTTCAGTTCGCGTTCATACAGGCCGCTGCTGATTCCGGCTAACTCAAGCTTCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCCCCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACTGGCGCGTGTCACCATATAGATTgcgc   <  2:116666/247‑1 (MQ=255)                                                                                                                                                                                                                     tGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGcgcg  >  1:79925/1‑251 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             GGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCG  >  NC_000913/82707‑83169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑