Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	78,533	A→C	M305L (ATG→CTG)	setA →	sugar exporter SetA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	78,533	0	A	C	100.0%	19.3 / NA	9	M305L (ATG→CTG)	setA	sugar exporter SetA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (2/7);  total (2/7)

GCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTT  >  NC_000913/78334‑78773                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 gCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCAttgtt                                                                                                                                                                                                <  2:183280/250‑1 (MQ=255)                    gAGTTAGGATTGCCAGACAAACTGGCGGGTTTTCTGATGGGGGCGGCAGCTGGGCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGGAAGCGGCGAATGATGGGCATAGCAGTGGCGGCAGGAGTACTGTTTTTCACCGGGTTGGTTTTTTTTTATAGCCGGCTGGCGTTGATGACGCCGCAACTTTTTTACGCTGTTTTTTTCGGCATTGTTGCGGGTTTTGGGGTTCTATg                                                                                                                                                                            >  2:240963/1‑251 (MQ=255)                          ggATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGGCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTGTCa                                                                                                                                                                      <  2:67904/251‑1 (MQ=255)                                                          ggggACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCt                                                                                                                                       <  1:50843/250‑1 (MQ=255)                                                                              aaaTACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGCCAGCGGCGAATGATGGTCCTAGCAGTGCCGGCCGGCGTACTGTTTTACACCGGATTGATTTTATTTCCTAGCCGTCTGACGTTGATGCCGCTGCCACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGt                                                                                                                  >  1:113343/1‑251 (MQ=255)                                                                                       cAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGTCGACTGATGTTCATAGCAGTGGCTGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTTGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTTTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTAcc                                                                                                         <  1:105619/251‑1 (MQ=255)                                                                                        aaTGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCg                                                                                                        <  1:59335/251‑1 (MQ=255)                                                                                                                                                                                     gattTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCg           <  1:446/251‑1 (MQ=255)                                                                                                                                                                                               caTAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGGTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCattatgtt  <  2:146050/249‑4 (MQ=255)                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 GCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTT  >  NC_000913/78334‑78773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑