Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 78,524 A→C N302H (AAT→CAT)  setA → sugar exporter SetA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091378,5240AC87.5% 16.7 / ‑5.2 8N302H (AAT→CAT) setAsugar exporter SetA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (1/6);  minor base T (1/0);  total (2/6)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCG  >  NC_000913/78334‑78764
                                                                                                                                                                                              |                                                                                                                                                                                                                                                
gCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCAttgtt                                                                                                                                                                                       <  2:183280/250‑1 (MQ=255)
                   gAGTTAGGATTGCCAGACAAACTGGCGGGTTTTCTGATGGGGGCGGCAGCTGGGCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGGAAGCGGCGAATGATGGGCATAGCAGTGGCGGCAGGAGTACTGTTTTTCACCGGGTTGGTTTTTTTTTATAGCCGGCTGGCGTTGATGACGCCGCAACTTTTTTACGCTGTTTTTTTCGGCATTGTTGCGGGTTTTGGGGTTCTATg                                                                                                                                                                   >  2:240963/1‑251 (MQ=255)
                         ggATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGGCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTGTCa                                                                                                                                                             <  2:67904/251‑1 (MQ=255)
                                                         ggggACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCt                                                                                                                              <  1:50843/250‑1 (MQ=255)
                                                                             aaaTACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGCCAGCGGCGAATGATGGTCCTAGCAGTGCCGGCCGGCGTACTGTTTTACACCGGATTGATTTTATTTCCTAGCCGTCTGACGTTGATGCCGCTGCCACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGt                                                                                                         >  1:113343/1‑251 (MQ=255)
                                                                                      cAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGTCGACTGATGTTCATAGCAGTGGCTGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTTGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTTTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTAcc                                                                                                <  1:105619/251‑1 (MQ=255)
                                                                                       aaTGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCg                                                                                               <  1:59335/251‑1 (MQ=255)
                                                                                                                                                                                    gattTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCg  <  1:446/251‑1 (MQ=255)
                                                                                                                                                                                              |                                                                                                                                                                                                                                                
GCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCG  >  NC_000913/78334‑78764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑