Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	78,520	C→A	F300L (TTC→TTA)	setA →	sugar exporter SetA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	78,520	0	C	A	88.9%	19.7 / ‑5.3	9	F300L (TTC→TTA)	setA	sugar exporter SetA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (2/6);  minor base T (1/0);  total (3/6)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCG  >  NC_000913/78273‑78764                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     ggATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGGACTGTTTTACACCGGATTGATTTTAttt                                                                                                                                                                                                                                                   >  1:44249/1‑251 (MQ=255)                                                              gCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCAttgtt                                                                                                                                                                                       <  2:183280/250‑1 (MQ=255)                                                                                 gAGTTAGGATTGCCAGACAAACTGGCGGGTTTTCTGATGGGGGCGGCAGCTGGGCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGGAAGCGGCGAATGATGGGCATAGCAGTGGCGGCAGGAGTACTGTTTTTCACCGGGTTGGTTTTTTTTTATAGCCGGCTGGCGTTGATGACGCCGCAACTTTTTTACGCTGTTTTTTTCGGCATTGTTGCGGGTTTTGGGGTTCTATg                                                                                                                                                                   >  2:240963/1‑251 (MQ=255)                                                                                       ggATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGGCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTGTCa                                                                                                                                                             <  2:67904/251‑1 (MQ=255)                                                                                                                       ggggACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCt                                                                                                                              <  1:50843/250‑1 (MQ=255)                                                                                                                                           aaaTACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGCCAGCGGCGAATGATGGTCCTAGCAGTGCCGGCCGGCGTACTGTTTTACACCGGATTGATTTTATTTCCTAGCCGTCTGACGTTGATGCCGCTGCCACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGt                                                                                                         >  1:113343/1‑251 (MQ=255)                                                                                                                                                    cAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGTCGACTGATGTTCATAGCAGTGGCTGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTTGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTTTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTAcc                                                                                                <  1:105619/251‑1 (MQ=255)                                                                                                                                                     aaTGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCg                                                                                               <  1:59335/251‑1 (MQ=255)                                                                                                                                                                                                                                                  gattTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCg  <  1:446/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     GGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCG  >  NC_000913/78273‑78764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑