Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 74,308 T→C I72V (ATA→GTA)  thiP ← thiamine ABC transporter membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091374,3080TC94.9% 121.3 / ‑5.7 39I72V (ATA→GTA) thiPthiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (18/19);  minor base G (0/2);  total (18/21)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GGCAGATTAAAAAACACATGGGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTATGACAGAGAGCAGTGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCGCTAATCCATGCCTGACGTTGTGCCGCCA  >  NC_000913/74067‑74552
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    
ggtaaagtAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGCCCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGACTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACagagag                                                                                                                                                                                                                                             <  2:145998/244‑1 (MQ=255)
    gATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAgc                                                                                                                                                                                                                                         <  1:134292/251‑2 (MQ=255)
        aaaaaaTACGTGCGCCAGCAAAATACCGTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGATGAATATCGCGGGTACGACAGAGAGCAGCGCCg                                                                                                                                                                                                                                     <  1:166689/251‑1 (MQ=255)
                           aaaaTACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGa                                                                                                                                                                                                                   >  1:43393/1‑250 (MQ=255)
                            aaaTACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGa                                                                                                                                                                                                                   >  1:21275/1‑249 (MQ=255)
                                   ttGCAGGCCGTAGGGCGAAAAGGTACACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTCAGAATGCAGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATCGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGACAGAAGGAGAAgc                                                                                                                                                                                                          >  2:28366/1‑251 (MQ=255)
                                      cAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGACGGAGAAgcgc                                                                                                                                                                                                        >  2:70027/1‑250 (MQ=255)
                                       aGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGcac                                                                                                                                                                                                      >  1:246057/1‑251 (MQ=255)
                                              aGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGCATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGc                                                                                                                                                                                               >  2:131905/1‑251 (MQ=255)
                                                    aaaaGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCcac                                                                                                                                                                                            >  2:21346/1‑248 (MQ=255)
                                                       aGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAg                                                                                                                                                                                      <  1:106467/251‑1 (MQ=255)
                                                                       gAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTg                                                                                                                                                                      <  1:248979/251‑1 (MQ=255)
                                                                                                  gccCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTcc                                                                                                                                           >  1:218342/1‑251 (MQ=255)
                                                                                                          gACCATAGAAGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGTGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAgg                                                                                                                                   >  1:75194/1‑251 (MQ=255)
                                                                                                                 gACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCgaga                                                                                                                             >  1:77781/1‑250 (MQ=255)
                                                                                                                      tAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGCTTAAGGTCATTGCGCAAAGACGCAACAGCGCCAGCCGACCCGGACAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGCGAAGCGCACCACATGCCACAGATCGCTGTCCTGCCAGACTGCCACCCAGTCATCCAGCGGCGCGTTCCACCACAGGGCGAGACAcgcc                                                                                                                       >  2:270706/1‑251 (MQ=255)
                                                                                                                       aaGAATGCCGCAAAAAGCAGCCAGCACAGGGTGGATTACGCTCATTGCGCACAGAAGCAACAGAGCCAGCCCACCCGGAAAGAGCCTGCGATAGCGCGCGCGGGCGAGGAATATCCCGGGTACGACAGCGAGCAGCGCCGAGAGAACCGCATGCCAGAAGGAGCAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGTCGCGTTCCACCACCGGGCGAGAAAcgccg                                                                                                                      >  2:27944/1‑251 (MQ=255)
                                                                                                                         gAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACgccgcc                                                                                                                    <  1:25216/251‑1 (MQ=255)
                                                                                                                                gAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCa                                                                                                              >  2:264613/1‑250 (MQ=255)
                                                                                                                                         aaccaggacCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATAGCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTacca                                                                                                    <  2:160635/244‑1 (MQ=255)
                                                                                                                                                     gaggaTTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGtggtgg                                                                                        >  1:141302/1‑251 (MQ=255)
                                                                                                                                                      aggaTTAAGGTCAGTGCGCACAGACGCAACAGCGTCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATTCCACAGATAGCGGTCCTGCCAGACTGCCACCCAGTCAGCCTGCGGCGCGTGCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCTCCAGCGTGGTGgc                                                                                       <  1:178211/251‑1 (MQ=255)
                                                                                                                                                            aaGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTac                                                                                 <  2:245089/251‑1 (MQ=255)
                                                                                                                                                             aGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTac                                                                                 >  1:9665/1‑250 (MQ=255)
                                                                                                                                                               gtcattgcgcacagctccaccAGCGCTAGCCGACCCAGAACGCGACTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGAACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCCGACGGAGAAGCGCACCACATGCCCCAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCACGTTCCACAACAGGGCGAGAAACGCCGCCAGCGCACACGCTACCACCAGCGTGGGGGCGCTTACAcc                                                                              >  2:162420/21‑251 (MQ=255)
                                                                                                                                                                  aTTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTgg                                                                           <  2:235720/251‑1 (MQ=255)
                                                                                                                                                                              cGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGcc                                                               <  1:4315/251‑1 (MQ=255)
                                                                                                                                                                                cAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCTCTAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCgg                                                             <  2:113565/251‑1 (MQ=255)
                                                                                                                                                                                      cgcCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAAtt                                                        >  2:35928/1‑250 (MQ=255)
                                                                                                                                                                                       gcCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCTCCAGCGTGGTGGCGCTTACACCTGGAATTAACGAGCCGGTAATTaa                                                      <  1:216553/251‑1 (MQ=255)
                                                                                                                                                                                               aCCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGTGAATTAACGGCTGAc                                              <  1:207181/251‑1 (MQ=255)
                                                                                                                                                                                                  cGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGgc                                           <  1:260341/251‑1 (MQ=255)
                                                                                                                                                                                                               cGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCg                              <  1:119191/251‑1 (MQ=255)
                                                                                                                                                                                                               cGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCg                              <  1:264613/251‑1 (MQ=255)
                                                                                                                                                                                                                    gagCGCGCATGCGAGGAATATCGCGGGTAGGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGATAAGCGCACCACATGCCCCAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGGGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTCACGGCTGACGGCGCGTTGCCATTCGCTAAt                         <  1:143472/251‑1 (MQ=255)
                                                                                                                                                                                                                              gCGAGGAATAGCGCGGGGAGCACAGAGAGCAGCGCCGAGGAAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGAGAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCGCTAATCCATGCCTGa               <  2:178432/251‑1 (MQ=255)
                                                                                                                                                                                                                                gAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCGCTAATCCATGCCTGACg             >  1:57147/1‑251 (MQ=255)
                                                                                                                                                                                                                                     atatCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCGCTAATCCATGCCTGACGTtgtg        <  1:21346/251‑1 (MQ=255)
                                                                                                                                                                                                                                           cgGGTACGACAGAGAGCCGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCGCTAATCCATGCCTGACGTTGTGCCGCCa  <  2:101873/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    
GGCAGATTAAAAAACACATGGGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTATGACAGAGAGCAGTGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCGCTAATCCATGCCTGACGTTGTGCCGCCA  >  NC_000913/74067‑74552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑