Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	74,087	G→C	A145A (GCC→GCG)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	74,087	0	G	C	92.1%	115.2 / 2.7	38	A145A (GCC→GCG)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base C (13/22);  total (15/23)
Fisher's exact test for biased strand distribution p-value = 5.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.28e-01

TGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCGGGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCAGATTAAAAAACACATGG‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTATGACAGAGAGCAGTGCCG  >  NC_000913/73849‑74325                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                tGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAAt                                                                                                                                                                                                                                     >  2:66468/1‑250 (MQ=255)  ggttgCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATa                                                                                                                                                                                                                                    <  1:199553/246‑1 (MQ=255)          gCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTTGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAg                                                                                                                                                                                                                            <  1:88452/250‑1 (MQ=255)                     cccAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCg                                                                                                                                                                                                                 >  1:200403/1‑250 (MQ=255)                        aGCGATAGAACGGTGGCACAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCAATCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCAGGGCGGCAAGCTGACGTTGTTCCCCGGGAATGGTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGGAGATTAAAAAATA‑AGGGCGCCAGCAAAATACCTTTCAGGACGTCGGGCaaaa                                                                                                                                                                                                              >  2:260341/1‑250 (MQ=255)                               tcACGGTGGAGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGGTCGCCGGGAATGTTTTCCAGTGCCTGGCGGAATAAGGGGCCCGCCATCGGTGGATTAAAAAATACGTGC‑GCACGCAAAATACCTTGCCGGCCGTAGGGGGAAAAgggcccc                                                                                                                                                                                                      >  2:91350/2‑246 (MQ=255)                                cACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCATCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACt                                                                                                                                                                                                     <  1:30140/251‑1 (MQ=255)                                   ggTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCa                                                                                                                                                                                                  <  1:115670/251‑1 (MQ=255)                                    gTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAg                                                                                                                                                                                                 <  1:69645/251‑1 (MQ=255)                                            gCTGGGGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAAGAAGCGGCTCGCCAGCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCATGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCg                                                                                                                                                                                         <  2:239166/251‑1 (MQ=255)                                                     aCAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGACGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGc                                                                                                                                                                                   >  1:113565/1‑248 (MQ=255)                                                           cATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTg                                                                                                                                                                          <  2:74792/251‑1 (MQ=255)                                                             aaaaGAGAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTTGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTgcc                                                                                                                                                                        <  2:192086/250‑1 (MQ=255)                                                                 gATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTgccagcc                                                                                                                                                                    <  1:37524/251‑1 (MQ=255)                                                                        gcagcaACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTg                                                                                                                                                             <  1:209433/251‑1 (MQ=255)                                                                         cagcaACCGGCGGGATTTGTCGCCGTAACCACGGCCAGTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTgg                                                                                                                                                            <  2:218017/251‑1 (MQ=255)                                                                                          tGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTaa                                                                                                                                            <  2:104693/250‑1 (MQ=255)                                                                                             cGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCCGCCGCGCATCCCAAGCTGGGCGGCAAGATGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAAt                                                                                                                                        >  1:135052/1‑251 (MQ=255)                                                                                                            cATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGcaac                                                                                                                         <  2:38912/251‑4 (MQ=255)                                                                                                              ttCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCCGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAAcc                                                                                                                        >  2:233258/1‑250 (MQ=255)                                                                                                                  acgaAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAgcac                                                                                                                   <  1:27196/251‑1 (MQ=255)                                                                                                                   cgaAGCGGACAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGTGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCAcc                                                                                                                  >  1:195042/1‑251 (MQ=255)                                                                                                                                  gccagccgcGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTc                                                                                                   <  1:47971/243‑1 (MQ=255)                                                                                                                                      gccgcGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTg                                                                                               <  2:34322/247‑1 (MQ=255)                                                                                                                                                ccAAGGTGGGCGGCAAGCTGGCGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTGGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGAGTAAGGGCCTTGCGCACAGACg                                                                                     <  2:172917/251‑1 (MQ=255)                                                                                                                                                ccAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACg                                                                                     >  2:59373/1‑251 (MQ=255)                                                                                                                                                          cggcAAGTTGACGCTGTTCGCCGGGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCAGATTAAAAAACACATGG‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGcc                                                                           >  2:235561/1‑251 (MQ=255)                                                                                                                                                          cggcAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGcc                                                                           <  1:140141/251‑1 (MQ=255)                                                                                                                                                             cAAGTTGACGCTGTTCGCCGGGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCAGATTAAAAAACACATGG‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGc                                                                        <  1:235561/251‑1 (MQ=255)                                                                                                                                                                               cGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCAATCGGAAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGAAGGCCGTAGGGCGAAAAGGGCCACTACAGAACGAGCGATAGGAAGAGAGTTGCAAGCCAGCCCTGGCGACCATAGAAGCTAAGAATGCCGAAAAAAGCAACCAGCAACGGGAGGATTAAGGGCATTGCGCACAGACGCAACAGCGCGAGACGACACGGAAAgagac                                                        >  1:52714/1‑245 (MQ=255)                                                                                                                                                                                   aaTGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGAt                                                  >  1:45636/2‑251 (MQ=255)                                                                                                                                                                                          tCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAgcgc                                           >  1:160635/1‑251 (MQ=255)                                                                                                                                                                                                        taataaGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAat                              >  1:108403/1‑250 (MQ=255)                                                                                                                                                                                                          ataaGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATc                           <  1:66468/251‑1 (MQ=255)                                                                                                                                                                                                                        aTCGGTAGATTAAAAAATACGTGC‑GCAAGCAAAAAACCTTGCAGGCCGCAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGAGGTCCCAGCCAGCCCTGGCGACCAGAAACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGTAGGATTAGGGTCATTGCGCACAGACGCACCAGCCCCGGCCGACCCGGAAAGCGCCCGCGATCGAGCGCGCCGGCGCGGAATATCGCggggacgac                >  2:219409/1‑242 (MQ=255)                                                                                                                                                                                                                           ggtaaagtAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGCCCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGACTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACagagag          <  2:145998/244‑1 (MQ=255)                                                                                                                                                                                                                               gATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAgc      <  1:134292/251‑2 (MQ=255)                                                                                                                                                                                                                                   aaaaaaTACGTGC‑GCCAGCAAAATACCGTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGATGAATATCGCGGGTACGACAGAGAGCAGCGCCg  <  1:166689/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                TGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCGGGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCAGATTAAAAAACACATGG‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTATGACAGAGAGCAGTGCCG  >  NC_000913/73849‑74325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑