| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 74,009 | T→C | Q171Q (CAA→CAG) | thiP ← | thiamine ABC transporter membrane subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 74,009 | 0 | T | C | 95.7% | 135.2 / ‑2.3 | 48 | Q171Q (CAA→CAG) | thiP | thiamine ABC transporter membrane subunit |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (17/28); total (19/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.79e-01 | |||||||||||
CACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCGGGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCAGATTAAAAAACACATGG‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGC > NC_000913/73764‑74255 | cacaCCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAAATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGCTTTGTCGCCGTAAACACGGCCATTCGACGAAGCGGAAAAAAAGCCAGCCGCGCCTCCCACGCTGGGCGGCAAGCTGACg > 1:166602/1‑251 (MQ=255) acacCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACgt > 1:235720/1‑250 (MQ=255) cacCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACgtt < 1:200975/251‑3 (MQ=255) cATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGAGCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCAGTCGACGAAGCGTAAAAAATGCTAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACgttgtt < 1:15866/251‑1 (MQ=255) atcgCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGGTTTGTCGCCGTAACCACGGCCATTCGAAGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGGCGTTGGTCGCCGGGGATGTTTTcc > 2:47971/4‑249 (MQ=255) ccGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAATGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGcc < 2:186676/251‑1 (MQ=255) cgcgcgGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCt < 1:117618/251‑1 (MQ=255) gCAGGATCGTAGTCGTAACTCAGCGCATGATAGATTGCCAGCTCGATAGTGGTTGCCTGAGGACCGCCACCCAGCGATCCCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGCCGGGATTTGTCGCCGTAACCACGGCCATTAGCAGAAGCGGAAAAAATACCAGCCGCGCATACCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGGCTGTTTTCCACTGCCTGGAgac > 1:167935/1‑248 (MQ=255) cGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGcc < 1:36085/251‑1 (MQ=255) tAACTCAGCGCCTGATAGCTTGCCAGCTCGATAGCGGTTGCCTGCGGACCGCCGACCAGCGATAGCACGGTGGCGAAGCTGGCGCAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCCCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCCGCCGCGCACCCCAAGCTGGGCCGCAACCTGAAGTTGTTCGCCCGGACTGTTTTCCAGCGGCTTGAGTAATAAGCGGCCCGcccc > 2:22548/1‑249 (MQ=255) tAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGACGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCCTCCCAAGCTGGGCGGCAAGCTGACGTTGTTCCCCGGGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCCCGCCac > 2:134292/1‑250 (MQ=255) aaCTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATc < 2:105725/251‑1 (MQ=255) gcCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGAtt > 1:101873/1‑251 (MQ=255) tGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCACCGGTAGATTaa > 2:140141/1‑250 (MQ=255) gATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTaaaa < 1:265232/251‑1 (MQ=255) aGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTaaaaaa < 1:211727/250‑1 (MQ=255) cgagaGTGGTAGCCTGCGGACCGCCGCCCAGCGATAGCTCGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGGGCAGCAACCAGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCGGACGTTGATCGCCGGGAATGTTTTCCAGGGGCTGGAGTAATAAGCGGCTCGCGATTGGTTGATTaaaaaagtcgtgcgccag < 2:106699/246‑13 (MQ=255) tGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAAt > 2:66468/1‑250 (MQ=255) ggttgCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATa < 1:199553/246‑1 (MQ=255) gCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTTGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAg < 1:88452/250‑1 (MQ=255) aCCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCAt > 1:143631/1‑204 (MQ=255) aCCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCAt < 2:143631/204‑1 (MQ=255) cccAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCg > 1:200403/1‑250 (MQ=255) aGCGATAGAACGGTGGCACAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCAATCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCAGGGCGGCAAGCTGACGTTGTTCCCCGGGAATGGTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGGAGATTAAAAAATA‑AGGGCGCCAGCAAAATACCTTTCAGGACGTCGGGCaaaa > 2:260341/1‑250 (MQ=255) tcACGGTGGAGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGGTCGCCGGGAATGTTTTCCAGTGCCTGGCGGAATAAGGGGCCCGCCATCGGTGGATTAAAAAATACGTGC‑GCACGCAAAATACCTTGCCGGCCGTAGGGGGAAAAgggcccc > 2:91350/2‑246 (MQ=255) cACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCATCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACt < 1:30140/251‑1 (MQ=255) ggTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCa < 1:115670/251‑1 (MQ=255) gTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAg < 1:69645/251‑1 (MQ=255) gCTGGGGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAAGAAGCGGCTCGCCAGCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCATGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCg < 2:239166/251‑1 (MQ=255) aCAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGACGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGc > 1:113565/1‑248 (MQ=255) cATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTg < 2:74792/251‑1 (MQ=255) aaaaGAGAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTTGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTgcc < 2:192086/250‑1 (MQ=255) gATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTgccagcc < 1:37524/251‑1 (MQ=255) gcagcaACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTg < 1:209433/251‑1 (MQ=255) cagcaACCGGCGGGATTTGTCGCCGTAACCACGGCCAGTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTgg < 2:218017/251‑1 (MQ=255) tGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTaa < 2:104693/250‑1 (MQ=255) cGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCCGCCGCGCATCCCAAGCTGGGCGGCAAGATGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAAt > 1:135052/1‑251 (MQ=255) cATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGcaac < 2:38912/251‑4 (MQ=255) ttCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCCGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAAcc > 2:233258/1‑250 (MQ=255) acgaAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAgcac < 1:27196/251‑1 (MQ=255) cgaAGCGGACAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGTGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCAcc > 1:195042/1‑251 (MQ=255) gccagccgcGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTc < 1:47971/243‑1 (MQ=255) gccgcGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTg < 2:34322/247‑1 (MQ=255) ccAAGGTGGGCGGCAAGCTGGCGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTGGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGAGTAAGGGCCTTGCGCACAGACg < 2:172917/251‑1 (MQ=255) ccAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACg > 2:59373/1‑251 (MQ=255) cggcAAGTTGACGCTGTTCGCCGGGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCAGATTAAAAAACACATGG‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGcc > 2:235561/1‑251 (MQ=255) cggcAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGC‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGcc < 1:140141/251‑1 (MQ=255) cAAGTTGACGCTGTTCGCCGGGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCAGATTAAAAAACACATGG‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGc < 1:235561/251‑1 (MQ=255) | CACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCGGGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCAGATTAAAAAACACATGG‑GCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGC > NC_000913/73764‑74255 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |