Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	73,763	G→A	C253C (TGC→TGT)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	73,763	0	G	A	100.0%	103.7 / NA	30	C253C (TGC→TGT)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/13);  total (17/13)

GCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGC  >  NC_000913/73516‑74002                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                gCAACGAGGTCCCCAGCGCCTGCCACAGCACCGGTTGTGCCGCCACTCCCGCCAACTGGCGATTTAGCCCATCGCCGACCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAacac                                                                                                                                                                                                                                              <  1:24006/251‑1 (MQ=255)         gTCCACAGCGCCTGCCACAGCACCGGTTGGGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTg                                                                                                                                                                                                                                      <  1:38646/251‑1 (MQ=255)                        cacaGCCCCGGTTGGCCCACCTCTTCCGGCAACGGGCGATTTAGCCCAGCGACGACCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCAt                                                                                                                                                                                                                       <  2:209332/251‑1 (MQ=255)                                  gTTGTGCCAGAACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCGGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCCATGGCCTTACTCACTCGCTGACTCAGCAGCACCAACCCGCGGCAACACACCACATGGATCAGCGCCCGCCTCGCCGCGCgg                                                                                                                                                                                                             >  2:207181/1‑251 (MQ=255)                                                           gCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAgcg                                                                                                                                                                                    <  2:113269/251‑1 (MQ=255)                                                            caatttagaccATCGACGATTACCGCCACCAACGGTAGCAGCAACAGCAGCAGCGCCAGCACAAGTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTGCGGGTCGCGCCAGCCTTGCAGCATCGGGGGGCCTGGCGCAATGGCCTTACTCAATAGCTGCCTCAGCCGCACCAGCCCGATGCAACTCACCATCTGGATCACCACCAGCACCGCCTCGCGGGCAGGAGCGCACTCGGAACTCAgcg                                                                                                                                                                                    >  2:143472/10‑250 (MQ=255)                                                             gATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGcc                                                                                                                                                                                  >  2:211727/1‑251 (MQ=255)                                                                      caccggcgaTCACCGCCAGCAACGGTCGCAGCAACAGCAGCAGCGCCAGCACAATTAACCCCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGAtt                                                                                                                                                                         <  1:145350/245‑1 (MQ=255)                                                                            cgagcACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCAGCGGGATCAGCGCCAGCATCGCCGGGCGGGCAGGATCGTAGTCGTAACTCAGCGCCGGATAGATTGCCAGc                                                                                                                                                                   <  1:170855/247‑1 (MQ=255)                                                                               ccACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCg                                                                                                                                                                <  1:52532/250‑1 (MQ=255)                                                                                cACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCg                                                                                                                                                                >  1:74792/1‑250 (MQ=255)                                                                                              gTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCTCAAATGCGGCTATGCAGACGTTCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGc                                                                                                                                                 <  1:102806/251‑1 (MQ=255)                                                                                               tGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCGCGATAGCGGTTGCCTGCg                                                                                                                                                >  2:115670/1‑251 (MQ=255)                                                                                                 gcagcaACAGCAGCAGCGCCAACACAATTCACAACGTAACGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCCATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGAGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTAAGCGCCTGATAGATTGCCAGATCGATAGTGGTTGCCTGCgg                                                                                                                                               >  2:259711/1‑250 (MQ=255)                                                                                                           cagcagcGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGt                                                                                                                                                        >  2:138781/1‑231 (MQ=255)                                                                                                           cagcagcGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGt                                                                                                                                                        <  1:138781/231‑1 (MQ=255)                                                                                                                 cgcCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGAt                                                                                                                               >  1:178432/1‑250 (MQ=255)                                                                                                                     aGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACAACATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCCGCGCCTGATATATTGCCAGCTCGCTAGTGGTTGCCTGCGGACCCCCGCCCCGAGATAGCAc                                                                                                                          >  2:222167/1‑251 (MQ=255)                                                                                                                          aaTTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCGCAGCGATAGCACGGTgg                                                                                                                     <  1:223794/251‑1 (MQ=255)                                                                                                                                        cGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCTCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCTCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCTCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGaa                                                                                                        <  1:259086/250‑1 (MQ=255)                                                                                                                                          cAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACa                                                                                                     <  1:44756/251‑1 (MQ=255)                                                                                                                                                   cTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATaa                                                                                             >  2:4315/1‑250 (MQ=255)                                                                                                                                                           aCGATCGACCGGGTCGCGCAAGACTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGCGGCGAAGCTGGCGAAACAGAGCATAACGATAAgtg                                                                                    >  2:25216/1‑249 (MQ=255)                                                                                                                                                                                gCCTGGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTc                                                               <  2:94499/251‑1 (MQ=255)                                                                                                                                                                                                         gcAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCTTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAATTGCAGCAGCGGGCTGGATTTGTCGCCGTAACCACGGCCATTCGACGaa                                      <  1:166586/251‑1 (MQ=255)                                                                                                                                                                                                                         aTCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATACATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGCTTTGTCGCCGCAACCACGGCCCTTCGACGAAGCGGAAAAAATGcccg                      >  2:216553/1‑249 (MQ=255)                                                                                                                                                                                                                              tgactccgcAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGccgcg                 >  1:101377/9‑247 (MQ=255)                                                                                                                                                                                                                                     gcAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATccc           >  1:245089/2‑250 (MQ=255)                                                                                                                                                                                                                                      cAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCa          >  1:38912/1‑250 (MQ=255)                                                                                                                                                                                                                                       aGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCCTTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCaac        >  2:209433/1‑250 (MQ=255)                                                                                                                                                                                                                                             aacccGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGggc  >  1:104693/3‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                GCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGC  >  NC_000913/73516‑74002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑