Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	73,445	C→A	L359L (CTG→CTT)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	73,445	0	C	A	100.0%	82.0 / NA	29	L359L (CTG→CTT)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/7);  total (22/7)

ACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAA‑TCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACG  >  NC_000913/73201‑73672                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                    aCTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAA‑TCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTcg                                                                                                                                                                                                                               >  1:145998/1‑251 (MQ=255)    gACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAA‑TCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTcgcg                                                                                                                                                                                                                             >  2:30629/1‑250 (MQ=255)                        gtgttaatatGGCGCTTCGGGTTTTCCAGTACTTTCAGCGAAAAAGGGTTCGCCATTACCGCATTGGTGAAAA‑GCACAATGCGGTCAGAAGATGGCGGAAGGCCAATGT‑TGTTGTTGAGAAGTAAAAAGAAGCCGGTAGCCAGCACAACCCCCGGCAGGGCGAGAAGCAACATGCCGCTCACCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGcatcat                                                                                                                                                                                                        <  1:257208/245‑1 (MQ=255)                                     gcATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAA‑TCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAAAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACg                                                                                                                                                                                           >  1:48206/1‑251 (MQ=255)                                      catcggtttgtcgattattttCAGCGCATAAGGGTTCGCCTTTACCTCATTG‑TGAAAAATCAAAATCCCGTCAGCAGATGGGGGCGGGCC‑ATGGGTGTTGTTGAGCAGTAAAAAAAGGCCGGTGGCCAGCAAAATCCCGGGCATGGCGAGGATAAACGGCCCGCTCACCCCCAGCGCCTGACCCGCCAGCATTTTCGGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGc                                                                                                                                                                                          <  2:167515/233‑1 (MQ=255)                                         cGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAA‑TCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGcac                                                                                                                                                                                        >  1:209332/1‑250 (MQ=255)                                                                 aTAAGGGATCGCCATTAACGCATTGGTGAAAG‑TCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATAc                                                                                                                                                               >  2:117618/1‑251 (MQ=255)                                                                 aTAAGGGATCGCCATTAACGCATTGGTGAAAA‑TCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATAc                                                                                                                                                               >  1:113269/1‑251 (MQ=255)                                                                    ggggATCCCCATTAACGCATTGGTGAAAA‑TCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCACCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCCCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCa                                                                                                                                                            <  1:88349/250‑1 (MQ=255)                                                                          cgcattaaacgcttgggtaaaaa‑TCACAATCCCTTAAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCACCAAAACCCCCGGCAGGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAgg                                                                                                                                                      <  1:179583/232‑1 (MQ=255)                                                                          cGCCATTAACGCATTGGTGAAAA‑TCACCATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGCTCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAgg                                                                                                                                                      >  2:30140/1‑251 (MQ=255)                                                                               ttAACGCATTGGTGAAAA‑TCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCcac                                                                                                                                                 >  2:36085/1‑251 (MQ=255)                                                                                                                                        gttgttGAGCAGAAAAAAGAAGCCGGGAGCCAGCACAACCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTagcc                                                                                          <  1:95814/251‑4 (MQ=255)                                                                                                                                                  aGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATcg                                                                                     >  1:193939/1‑246 (MQ=255)                                                                                                                                                    tAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATAGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCAAAGTCCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTCCCGCCAGCGCAATACGCAACGAGGACCACCGCGCCTACCACAGCACCGGCTGTGCCCGCACTTCCGGCAACTGGCGATTAACCCCATcgacgaccc                                                                              >  2:102806/1‑248 (MQ=255)                                                                                                                                                         agaagCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTCGCCCATCGACGATCACCGcc                                                                         >  2:145350/1‑251 (MQ=255)                                                                                                                                                          gaagCCGGTAGCCAGCACAATCCCCGGCATGGTGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGCTTTAGCCCATCGACGATCACCGccc                                                                        >  2:27196/1‑250 (MQ=255)                                                                                                                                                                                            aGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTCGCAGCAACAGCAGCAGCGCCAGcac                                      >  2:199553/1‑251 (MQ=255)                                                                                                                                                                                            aGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGAGCCCGACGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTAAGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCCGCACTTCCGGCAAATGGCGATGTAGCCCATCGCCGATCACCGCCAGAACCGGCGGCAGCAACAGCAGCAGCGCGAGcac                                      >  2:119191/1‑251 (MQ=255)                                                                                                                                                                                              gATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTACCCCGCCACCGAAGTTCGCGACTGCTCCATAGCCGCATCCTGGTCAGCACTCCGCACCATACCCCTGCCGCCAGCGCAATACGCAACGAGGACCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGcaca                                     >  1:263837/1‑250 (MQ=255)                                                                                                                                                                                               aTCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAAt                                   >  1:106699/1‑251 (MQ=255)                                                                                                                                                                                                  aaCATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGAGGCAGCAACAGCAGCAGCGCCAGCACAATTa                                 >  2:69645/1‑250 (MQ=255)                                                                                                                                                                                                   aCATGCCGCTCATCTCCAGCGCCTGAACCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGAATCATGGCCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCACCGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGCTTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACCATTAac                               >  2:166689/1‑251 (MQ=255)                                                                                                                                                                                                        ccGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTCACACCgt                          >  2:106467/1‑251 (MQ=255)                                                                                                                                                                                                                    agcgccTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCg              >  1:172917/5‑251 (MQ=255)                                                                                                                                                                                                                     gcccttcccccGCAAGCATTTTCTGCCGCGCCCGAATTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCAAAATACCCCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGGGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCgg             <  1:100753/243‑1 (MQ=255)                                                                                                                                                                                                                          ccgccctcccgctcttccTGCCGCGCCCAATGTTCGCGCCTCCCCCATAGCAGCATAATGGTCAGCACACCGCACAATACCCCTCCCGCCAGCGCAATACGCAACGAGGCCCACGGCGCCTGCCACAGCACCGGTTGTGCCAGCACTCCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATg        <  2:128462/234‑1 (MQ=255)                                                                                                                                                                                                                                gCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACg  >  2:44756/1‑251 (MQ=255)                                                                                                                                                                                                                                         ttCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATg        >  1:128462/1‑236 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                    ACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAA‑TCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACG  >  NC_000913/73201‑73672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑