Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	73,413	A→G	V370A (GTG→GCG)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	73,413	0	A	G	100.0%	79.9 / NA	27	V370A (GTG→GCG)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/8);  total (19/8)

TTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAA‑TCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGT  >  NC_000913/73170‑73648                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            tttAAGCGCGCCCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTACAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAA‑TCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTATCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGAc                                                                                                                                                                                                                                       <  2:203310/250‑1 (MQ=255)   tAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAA‑TCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGAccc                                                                                                                                                                                                                                     <  1:226151/250‑1 (MQ=255)   aaaGCGCGACCAGCCTTCAAGGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAA‑TCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCg                                                                                                                                                                                                                                    <  2:44298/250‑1 (MQ=255)                                aCTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAA‑TCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTcg                                                                                                                                                                                                       >  1:145998/1‑251 (MQ=255)                                   gACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAA‑TCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTcgcg                                                                                                                                                                                                     >  2:30629/1‑250 (MQ=255)                                                       gtgttaatatGGCGCTTCGGGTTTTCCAGTACTTTCAGCGAAAAAGGGTTCGCCATTACCGCATTGGTGAAAA‑GCACAATGCGGTCAGAAGATGGCGGAAGGCCAATGT‑TGTTGTTGAGAAGTAAAAAGAAGCCGGTAGCCAGCACAACCCCCGGCAGGGCGAGAAGCAACATGCCGCTCACCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGcatcat                                                                                                                                                                                <  1:257208/245‑1 (MQ=255)                                                                    gcATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAA‑TCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAAAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACg                                                                                                                                                                   >  1:48206/1‑251 (MQ=255)                                                                     catcggtttgtcgattattttCAGCGCATAAGGGTTCGCCTTTACCTCATTG‑TGAAAAATCAAAATCCCGTCAGCAGATGGGGGCGGGCC‑ATGGGTGTTGTTGAGCAGTAAAAAAAGGCCGGTGGCCAGCAAAATCCCGGGCATGGCGAGGATAAACGGCCCGCTCACCCCCAGCGCCTGACCCGCCAGCATTTTCGGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGc                                                                                                                                                                  <  2:167515/233‑1 (MQ=255)                                                                        cGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAA‑TCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGcac                                                                                                                                                                >  1:209332/1‑250 (MQ=255)                                                                                                aTAAGGGATCGCCATTAACGCATTGGTGAAAG‑TCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATAc                                                                                                                                       >  2:117618/1‑251 (MQ=255)                                                                                                aTAAGGGATCGCCATTAACGCATTGGTGAAAA‑TCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATAc                                                                                                                                       >  1:113269/1‑251 (MQ=255)                                                                                                   ggggATCCCCATTAACGCATTGGTGAAAA‑TCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCACCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCCCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCa                                                                                                                                    <  1:88349/250‑1 (MQ=255)                                                                                                         cgcattaaacgcttgggtaaaaa‑TCACAATCCCTTAAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCACCAAAACCCCCGGCAGGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAgg                                                                                                                              <  1:179583/232‑1 (MQ=255)                                                                                                         cGCCATTAACGCATTGGTGAAAA‑TCACCATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGCTCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAgg                                                                                                                              >  2:30140/1‑251 (MQ=255)                                                                                                              ttAACGCATTGGTGAAAA‑TCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCcac                                                                                                                         >  2:36085/1‑251 (MQ=255)                                                                                                                                                                       gttgttGAGCAGAAAAAAGAAGCCGGGAGCCAGCACAACCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTagcc                                                                  <  1:95814/251‑4 (MQ=255)                                                                                                                                                                                 aGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATcg                                                             >  1:193939/1‑246 (MQ=255)                                                                                                                                                                                   tAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATAGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCAAAGTCCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTCCCGCCAGCGCAATACGCAACGAGGACCACCGCGCCTACCACAGCACCGGCTGTGCCCGCACTTCCGGCAACTGGCGATTAACCCCATcgacgaccc                                                      >  2:102806/1‑248 (MQ=255)                                                                                                                                                                                        agaagCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTCGCCCATCGACGATCACCGcc                                                 >  2:145350/1‑251 (MQ=255)                                                                                                                                                                                         gaagCCGGTAGCCAGCACAATCCCCGGCATGGTGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGCTTTAGCCCATCGACGATCACCGccc                                                >  2:27196/1‑250 (MQ=255)                                                                                                                                                                                                                           aGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTCGCAGCAACAGCAGCAGCGCCAGcac              >  2:199553/1‑251 (MQ=255)                                                                                                                                                                                                                           aGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGAGCCCGACGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTAAGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCCGCACTTCCGGCAAATGGCGATGTAGCCCATCGCCGATCACCGCCAGAACCGGCGGCAGCAACAGCAGCAGCGCGAGcac              >  2:119191/1‑251 (MQ=255)                                                                                                                                                                                                                             gATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTACCCCGCCACCGAAGTTCGCGACTGCTCCATAGCCGCATCCTGGTCAGCACTCCGCACCATACCCCTGCCGCCAGCGCAATACGCAACGAGGACCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGcaca             >  1:263837/1‑250 (MQ=255)                                                                                                                                                                                                                              aTCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAAt           >  1:106699/1‑251 (MQ=255)                                                                                                                                                                                                                                 aaCATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGAGGCAGCAACAGCAGCAGCGCCAGCACAATTa         >  2:69645/1‑250 (MQ=255)                                                                                                                                                                                                                                  aCATGCCGCTCATCTCCAGCGCCTGAACCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGAATCATGGCCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCACCGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGCTTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACCATTAac       >  2:166689/1‑251 (MQ=255)                                                                                                                                                                                                                                       ccGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTCACACCgt  >  2:106467/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            TTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAA‑TCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGT  >  NC_000913/73170‑73648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑