Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	72,928	T→C	N532D (AAT→GAT)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	72,928	0	T	C	100.0%	103.6 / NA	30	N532D (AAT→GAT)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/14);  total (16/14)

TGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCC  >  NC_000913/72687‑73163                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            tgttTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCgg                                                                                                                                                                                                                                    >  2:257816/1‑251 (MQ=255)       ccTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGttt                                                                                                                                                                                                                              <  1:69035/251‑1 (MQ=255)              cAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATc                                                                                                                                                                                                                       >  1:53527/1‑251 (MQ=255)                     gACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTa                                                                                                                                                                                                                 >  2:226151/1‑250 (MQ=255)                        acCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCATTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAAca                                                                                                                                                                                                             <  1:230410/251‑1 (MQ=255)                                  gTGACGGCGGCGTCGTGGTGTGATCAACCCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCCCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAAc                                                                                                                                                                                                    >  2:132009/1‑250 (MQ=255)                                         cggcgtcgtggtgtgATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGagtagc                                                                                                                                                                                            >  1:167515/11‑251 (MQ=255)                                            cgtcgtggtgtgATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAACCGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACCGCAGAAAACAGagtagcagc                                                                                                                                                                                         <  2:108338/244‑1 (MQ=255)                                                           aaCGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTg                                                                                                                                                                          <  1:198313/250‑1 (MQ=255)                                                              gccgtCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGAcc                                                                                                                                                                       >  2:97812/5‑251 (MQ=255)                                                                        gTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCt                                                                                                                                                             <  2:55453/251‑1 (MQ=255)                                                                        gTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCt                                                                                                                                                             >  1:225379/1‑251 (MQ=255)                                                                                   gctggctgGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGAt                                                                                                                                                    >  1:22919/1‑249 (MQ=255)                                                                                      ggctggcGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAgg                                                                                                                                                 <  1:257816/249‑1 (MQ=255)                                                                                        ctggcGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCTGTCCTGGCTGCGATAGGAGcc                                                                                                                                             >  1:240663/1‑251 (MQ=255)                                                                                         gggcGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCa                                                                                                                                            <  2:100497/250‑1 (MQ=255)                                                                                             gTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGTGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCTCCGTCCTGGCTGCGATAGGAGACAAttt                                                                                                                                        <  1:215519/251‑1 (MQ=255)                                                                                                                      gcagGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGt                                                                                                               <  1:24194/251‑1 (MQ=255)                                                                                                                             aCTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGaaa                                                                                                        <  1:80390/251‑1 (MQ=255)                                                                                                                                  tACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAatcat                                                                                                    <  2:28690/250‑1 (MQ=255)                                                                                                                                                     gAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACACCGccacc                                                                                >  2:105813/1‑251 (MQ=255)                                                                                                                                                                       gccgcGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAACTCACCAATc                                                              >  2:100753/1‑251 (MQ=255)                                                                                                                                                                                   cGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCAACCACGCCAAAATCAACAATCGACAGCCCGc                                                    >  2:223794/1‑249 (MQ=255)                                                                                                                                                                                       aaGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAAGCGACAGCACGCAGGCaa                                              <  2:93806/251‑1 (MQ=255)                                                                                                                                                                                                   aTCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCt                                  >  2:21783/1‑251 (MQ=255)                                                                                                                                                                                                         aaaTGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCa                            <  2:118312/251‑1 (MQ=255)                                                                                                                                                                                                               tggtAAAGCCAGGTGATATCAGTCAGGTTTAACATCTCGCCCCGGTAGTTTTTCAAGCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGa                       <  2:95866/250‑1 (MQ=255)                                                                                                                                                                                                                         aGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGgc            >  1:75392/1‑251 (MQ=255)                                                                                                                                                                                                                          ggTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGgcc           >  2:95814/1‑250 (MQ=255)                                                                                                                                                                                                                                    gtcagtTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTcg  >  2:158149/1‑249 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            TGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCC  >  NC_000913/72687‑73163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑