Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,794	T→C	A329A (GCA→GCG)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,794	0	T	C	97.1%	112.5 / NA	34	A329A (GCA→GCG)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (12/21);  minor base A (1/0);  total (13/21)
Fisher's exact test for biased strand distribution p-value = 3.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTAT  >  NC_000913/64549‑65035                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   aGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAg                                                                                                                                                                                                                                               <  1:129805/251‑1 (MQ=255)                            ccgccAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGATCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAAcggcg                                                                                                                                                                                                                    >  2:120790/1‑251 (MQ=255)                                     atGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCCGTCACCGTCGCGCGTTCGAGGAAAAAAGGCATGATTTCAGGTTTATGGGAAATCTGCTTCACCAGCTCGCAATCTTTCAGGTTCTAAGTCGCCAGCGCCGGTGTATCTTCGGAGAAACGGCGGTACAAttt                                                                                                                                                                                                           >  2:208897/2‑251 (MQ=255)                                               gcggcACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGa                                                                                                                                                                                                <  1:231923/251‑1 (MQ=255)                                                              gaTTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCg                                                                                                                                                                                 <  1:203293/251‑1 (MQ=255)                                                                  aGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAg                                                                                                                                                                             <  1:246336/251‑1 (MQ=255)                                                                      gcgACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAAATTCGGCCATTCGATCCCACGGGGTTTCGATaacatt                                                                                                                                                                         >  2:220305/1‑246 (MQ=255)                                                                        gACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTc                                                                                                                                                                       <  2:52046/251‑1 (MQ=255)                                                                         aCATAACCAGCGCGGTGCATACGAGGGAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCGCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTGTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTGCGTCCATTCGATCCCACGGGTTATCGATAGATTTTcc                                                                                                                                                                      <  2:94827/251‑1 (MQ=255)                                                                         aCATAACAAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGACAATCTGCGTCACCAGCTCGCACTCTTTCAGGTTATCAGTAGCCCGCGCAGGTTTATCTTCGGCGAAACGGCGGG‑CCATTTCGTCCATTTTATCCCACGGGGTATCTATAGATTTTcc                                                                                                                                                                      >  2:53938/1‑251 (MQ=255)                                                                            tAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCCTTCGATCCCACGGGTTATCGATAGATTTTCCTTc                                                                                                                                                                   >  1:151641/1‑251 (MQ=255)                                                                                   cgcgGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAAt                                                                                                                                                            <  1:214615/251‑1 (MQ=255)                                                                                   cgcgGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGGTATCGATAGATTTTCCTTCTCCTAAt                                                                                                                                                            >  1:82465/1‑251 (MQ=255)                                                                                               gaggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑TAATTTCGTCCATTCGATCCCACGGGTTATCGATAGAATTTCCTTCGCCTACTAACTCCTGAGCg                                                                                                                                                >  2:90344/3‑251 (MQ=255)                                                                                                      aTAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGAcgggtt                                                                                                                                         >  1:226213/1‑246 (MQ=255)                                                                                                        agagATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTc                                                                                                                                        <  1:148857/250‑1 (MQ=255)                                                                                                         gagaTGACCAAACGCCGACACCGATTCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTAGATCGGTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCGTCGGCGAAACGGCGGT‑CGATTGCGTCCATTCGATCCCACGTGTGATCGATAGATTTTCCTTCGCCTAATAACGCCTGAGCgcagggttcta                                                                                                                                      <  2:178985/251‑11 (MQ=255)                                                                                                                            aCCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAAtt                                                                                                                   <  1:166707/251‑1 (MQ=255)                                                                                                                             ccgatcccccgTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTc                                                                                                                  <  1:70926/243‑1 (MQ=255)                                                                                                                                                                             cGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACTGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCATAATAACTCCTTAGCGTCGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGTATTTCAGCGCCTCGATACCTTCGATAATTAGCCGAcc                                                                   <  1:180493/250‑1 (MQ=255)                                                                                                                                                                             cGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGAcc                                                                   <  1:180482/250‑1 (MQ=255)                                                                                                                                                                                  aaaaaTGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGc                                                             >  2:242759/1‑251 (MQ=255)                                                                                                                                                                                   aaaaTGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGcc                                                            <  1:236712/251‑1 (MQ=255)                                                                                                                                                                                     aaTGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTg                                                          <  2:209172/251‑1 (MQ=255)                                                                                                                                                                                          cATGATTTCAGTTGTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTGAGCCTGGGCaa                                                     <  2:25253/251‑1 (MQ=255)                                                                                                                                                                                             gATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCaaaaa                                                  <  1:242759/251‑1 (MQ=255)                                                                                                                                                                                                ttCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAg                                                <  1:26468/250‑1 (MQ=255)                                                                                                                                                                                                           ggAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGttttt                                     <  2:191660/250‑1 (MQ=255)                                                                                                                                                                                                                      gTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCCATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTc                         >  1:106304/1‑251 (MQ=255)                                                                                                                                                                                                                                 gCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGCGCTCGCGCCACTcc               >  1:204400/1‑250 (MQ=255)                                                                                                                                                                                                                                      cTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTc          >  2:234046/1‑250 (MQ=255)                                                                                                                                                                                                                                          cAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTcactgt     <  2:235439/251‑6 (MQ=255)                                                                                                                                                                                                                                          cAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTcactgt     <  1:90426/251‑6 (MQ=255)                                                                                                                                                                                                                                             gTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTATCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCCGCTcactgtttt  >  1:66544/1‑243 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   AGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGT‑CAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTAT  >  NC_000913/64549‑65035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑