Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	72,325	T→C	V201V (GTA→GTG)	thiQ ←	thiamine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	72,325	0	T	C	100.0%	80.2 / NA	25	V201V (GTA→GTG)	thiQ	thiamine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/10);  total (15/10)

ATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGACAACGCGCTAACGCCACTCGCTGTCGCTGACCGCCGGAAAGCTCGCCCG  >  NC_000913/72083‑72548                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                atCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAgcg                                                                                                                                                                                                                         >  1:95342/1‑251 (MQ=255)       atTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTCTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGc                                                                                                                                                                                                                   >  1:25656/1‑251 (MQ=255)          ttGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACCAAACGCACTGTCCGTAGGACGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCAGTCGGCGACCACCACCGCGCGCGTGGCgct                                                                                                                                                                                                                >  2:198313/1‑249 (MQ=255)                   gTGGTTGGGGTTTAGGGGATTGTTTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCgcgcggc                                                                                                                                                                                                       <  2:177614/251‑4 (MQ=255)                        tGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCtt                                                                                                                                                                                                  >  1:70482/1‑251 (MQ=255)                                 ggggattgTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACtgt                                                                                                                                                                                         >  1:108338/8‑251 (MQ=255)                                    gattgTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTTAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACCCtgtgtg                                                                                                                                                                                      >  1:121472/5‑249 (MQ=255)                                     attgTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACtgtgtg                                                                                                                                                                                      >  1:222099/4‑248 (MQ=255)                                      ttttCTTGAGCGGGTTGCCTGGTGCGACGCTGACCGGTTCTTGTCGGGCCTACAAAACGCACTGTCCGTAGTTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCGTAAGCACTCGCCTTACCGCTCAACAACTCATGAGTCTTTCCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTAGCGATCCGCGCGGCATCTTCCACACtgtgtga                                                                                                                                                                                     <  2:1101/247‑4 (MQ=255)                                                 gggTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATa                                                                                                                                                                          >  1:221030/1‑250 (MQ=255)                                                                                     gCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCACTAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCAcc                                                                                                                                       >  1:32660/1‑249 (MQ=255)                                                                                         aCAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCg                                                                                                                                   <  1:58646/249‑1 (MQ=255)                                                                                         aCAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTc                                                                                                                                 >  1:93806/1‑251 (MQ=255)                                                                                               cgcactgtccGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGTATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCAtc                                                                                                                           <  2:133743/243‑1 (MQ=255)                                                                                                gcactgtccGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGCTCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCAtct                                                                                                                          >  1:17188/8‑251 (MQ=255)                                                                                                 cactgtccGTAGGTCGGATAAGGTGTTCACGCCGCATCGGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTGACCCTGCCAGGCGATGCGCCCGGCGGCGACCACCACCGAGCTCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACTTCTGTTTTTGCTGCGGGCAGCTCGTGCTCACCAGCGTCAGCAtctc                                                                                                                         <  1:167862/245‑1 (MQ=255)                                                                                                                    aaGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATc                                                                                                       <  1:39902/250‑1 (MQ=255)                                                                                                                    aaGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATc                                                                                                       <  2:70463/250‑1 (MQ=255)                                                                                                                                          cAGTGCATACTAACCCGTAATACCCAATAGTGCTGAAGCACTCGCCTTACCGCTCCACAACTCATTGTTCTTACCCTGCCAGGCGATGCTCCCGTCGGCGACCACCACCGAGCGCGTGACGATCCGCGCGGCCTCTTCCACACTGTGTGCCACCCTCAATACCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCg                                                                                 >  2:42762/1‑250 (MQ=255)                                                                                                                                                 tACTAACCCGTAATACCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGTTCGAGCGCCGATAACGGTTCactgagaaata                                                                          >  2:215519/1‑240 (MQ=255)                                                                                                                                                      aCCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCg                                                                    <  2:217954/251‑1 (MQ=255)                                                                                                                                                         cGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCt                                                                 >  2:24194/1‑251 (MQ=255)                                                                                                                                                          gTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGGCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACCCCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTg                                                                <  2:166446/251‑1 (MQ=255)                                                                                                                                                                                             gCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACgcgct                             >  1:241937/1‑251 (MQ=255)                                                                                                                                                                                                                        ccAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAGCGTCATTTTTTGCTGCGGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGAAGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGccag  <  2:217854/251‑3 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                ATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGACAACGCGCTAACGCCACTCGCTGTCGCTGACCGCCGGAAAGCTCGCCCG  >  NC_000913/72083‑72548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑