Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	72,117	A→G	intergenic (+2/+112)	yabI → / ← thiQ	DedA family protein YabI/thiamine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	72,117	0	A	G	100.0%	118.7 / NA	33	intergenic (+2/+112)	yabI/thiQ	DedA family protein YabI/thiamine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/21);  total (12/21)

GCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTT  >  NC_000913/71871‑72356                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                gcggcgAGTTTAAATGGTGGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGAtt                                                                                                                                                                                                                                             <  1:71777/251‑2 (MQ=255)                        gtggcgacggCGGTATTTGTGTGGGTTGGGGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGGTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATAGTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGc                                                                                                                                                                                                                      <  2:23784/242‑1 (MQ=255)                                   ggtatttgtgtgGGTTGGTGGCTGGCTTTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTTCCTGATGCGACGCtgaccg                                                                                                                                                                                                           <  2:235612/243‑6 (MQ=255)                                                      ggctggctGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACaa                                                                                                                                                                                        <  2:213352/251‑1 (MQ=255)                                                       gctggctgTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACaaa                                                                                                                                                                                       <  1:83658/251‑1 (MQ=255)                                                              gtgCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACt                                                                                                                                                                                <  2:79230/251‑1 (MQ=255)                                                                   ggCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACtgtc                                                                                                                                                                             <  2:156982/249‑4 (MQ=255)                                                                      ggTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAg                                                                                                                                                                        <  1:116095/251‑1 (MQ=255)                                                                                       aaagcaaccgatcGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCa                                                                                                                                                       <  1:111874/239‑1 (MQ=255)                                                                                                  tcGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCTCCCGTTGATGCCGGGGTATATCGATATTTTGCGTAAAGTGGTTGGGTTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATTCGACGCTGACCGCGTCTTATCAGGCCTACAAATCGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATct                                                                                                                                             <  1:44153/249‑2 (MQ=255)                                                                                                        aaGTCATTATTTGTCGGGCGGCTGTTTGTTGTGGCTGCCGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCCCCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAACCGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTg                                                                                                                                      <  2:4450/250‑1 (MQ=255)                                                                                                        aaGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTTTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCCTAGGTCGGATAAGGTGTTCACCCCCCCTCTGACAGTg                                                                                                                                      >  2:39902/2‑251 (MQ=255)                                                                                                        aaGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTg                                                                                                                                      <  2:66891/250‑1 (MQ=255)                                                                                                                         gcgGCCGTTTGTGGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATc                                                                                                                     <  2:143177/251‑1 (MQ=255)                                                                                                                              cGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGAGGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCaa                                                                                                                <  1:270568/251‑1 (MQ=255)                                                                                                                                   gttgtGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAgggc                                                                                                                                                                     >  2:7309/1‑191 (MQ=255)                                                                                                                                   gttgtGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTc                                                                                                                                                                     <  1:7309/193‑1 (MQ=255)                                                                                                                                        ggCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCGGGGGGAGGTAATTCGCCACCCGTTGATGCCGGGGGATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGAGTGTCTTGGGCGGGTTGCCTGATGCGAAGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGGTCACGCCGCATCTGACAGTGCATACTAAACCGTAATCCCCAATAGTGctga                                                                                                       >  2:37472/1‑247 (MQ=255)                                                                                                                                                     gATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTa                                                                                          <  1:182661/250‑1 (MQ=255)                                                                                                                                                            gctcgcggctgggtggCGCTGGTGGTTTTAAGTCGCCACCCGTTGATGCCGGTGTATATCGATATTTGGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGGGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTca                                                                                   <  2:198240/239‑1 (MQ=255)                                                                                                                                                              cATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTcaac                                                                                 >  1:133743/1‑250 (MQ=255)                                                                                                                                                                  ggCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTc                                                                            >  2:211640/1‑251 (MQ=255)                                                                                                                                                                       ggtggCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTg                                                                        <  1:2690/250‑1 (MQ=255)                                                                                                                                                                          ggCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTct                                                                    >  1:57819/1‑250 (MQ=255)                                                                                                                                                                                             cGCCCCCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATgc                                                  <  1:626/250‑1 (MQ=255)                                                                                                                                                                                             cGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGa                                                                                                                                                                  <  2:56296/138‑1 (MQ=255)                                                                                                                                                                                             cGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGa                                                                                                                                                                  >  1:56296/1‑138 (MQ=255)                                                                                                                                                                                                        gATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCg                                       >  1:222680/1‑250 (MQ=255)                                                                                                                                                                                                                     atCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAgcg                         >  1:95342/1‑251 (MQ=255)                                                                                                                                                                                                                           atTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTCTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGc                   >  1:25656/1‑251 (MQ=255)                                                                                                                                                                                                                              ttGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACCAAACGCACTGTCCGTAGGACGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCAGTCGGCGACCACCACCGCGCGCGTGGCgct                >  2:198313/1‑249 (MQ=255)                                                                                                                                                                                                                                       gTGGTTGGGGTTTAGGGGATTGTTTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCgcgcggc       <  2:177614/251‑4 (MQ=255)                                                                                                                                                                                                                                            tGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCtt  >  1:70482/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                GCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTT  >  NC_000913/71871‑72356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑