Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,899	A→G	A183A (GCA→GCG)	yabI →	DedA family protein YabI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,899	0	A	G	100.0%	63.2 / NA	26	A183A (GCA→GCG)	yabI	DedA family protein YabI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/19);  total (7/19)

CAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCC‑AGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATA  >  NC_000913/71674‑72121                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                 caGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGAcggcgggatttgtggggggtggtg                                                                                                                                                                                                         >  1:79230/1‑233 (MQ=255)  aGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTAAGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGGTGCTGCTGGCGACGGCGGGATTTGGGT‑GGGTtggtgg                                                                                                                                                                                                       >  1:70463/1‑251 (MQ=255)     aTGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGGTCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGGTTTTTTGT‑GGGTTTGtggctg                                                                                                                                                                                                    >  1:197781/1‑251 (MQ=255)                  tGGTCGGTCGCTTTGTTGTCCCGACGCGTCCGCTGGTGCCAATGGCGGCGGGCAGTATGGATCTGCCGGTGGCAAAATTCATTTCGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑CGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGGGTTTAAATGGTTGCTGCTGGCGACGGCGG‑GATTTGGTTGGGTTGGTgggtggcttttgtggcgg                                                                                                                                                                                       >  1:268400/1‑241 (MQ=255)                    gtcggtcgCTTTGTTAGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTTCC‑TGGGATTCTGGCGGGAGCGGCGATCGATATTCCCGCCGGAATGCAGAGCGGCGAGGTTAAAAGGTTGCTGCTGGCGAAGGCGGTATTTTTGT‑GGGGTGGGGGCTGGCTTTGCTggggggt                                                                                                                                                                                     >  1:217854/1‑246 (MQ=255)                                gttgGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCgg                                                                                                                                                                         <  1:47816/251‑1 (MQ=255)                                           gggaccGCTGGGGCCCATTGTGGCGGTGATGCTGGAGATGGCGGTGGCGAAATTCATTACGCCGAATATTCTGGCCTGCCTGCGGTGGCCTCTGTTTTGCTCCCTGCCGAGGG‑TGCTGGCGGGCGCGGTGATCGGTATTCCAGCGGGAATGCAGAGCGGCGAGTGTAAATGGTTGCTGCTGGGGACGGCGGTGTGTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGcaaccg                                                                                                                                                              <  2:61791/247‑6 (MQ=255)                                               ccGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGcaaccgatc                                                                                                                                                           <  1:229292/250‑9 (MQ=255)                                                                ggCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTg                                                                                                                                          <  1:15759/250‑1 (MQ=255)                                                                      aaTGCTGGATCTGCCGGTGGCAAAAGTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCGGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTcgcgcg                                                                                                                                   <  2:240090/251‑1 (MQ=255)                                                                                  gccggtgatacaaTTCATTACGCCGAATATTATCGGCTGCCGGCTGTGGCCACCGGTTTACTTCCTGCC‑GGGGATACTGGCGGGCGCGGCGATCGATATTCCAGGCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTGTGGCGCAGCGGTAAAGCACCCGATCGTTTAAGTCATTGTTTGTCGCGCGGCCG‑Attgtt                                                                                                                        <  2:268400/238‑1 (MQ=255)                                                                                            aaaTTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTTTGGCGCAGCGGGAAAGCAACCCATCGTTTTAGTCATTATTTTTTGCGCGGGCG‑TTTTTTGTTGCttgcgcc                                                                                                            >  1:217954/1‑245 (MQ=255)                                                                                                       gCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGGGCAGCGGGAAAGGAACCGATCGTTTTAGGCATTATTTTTCTCGCGGCCGGTTTGT‑GTGGGTGACGCCggtgttttttg                                                                                                 >  1:196775/1‑241 (MQ=255)                                                                                                                        gcctgcTGTGGCCACCGTTTTTCTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGgc                                                                                 <  2:211933/250‑1 (MQ=255)                                                                                                                               gtgGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtggt                                                                           <  2:144728/249‑1 (MQ=255)                                                                                                                                   ccaccgTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGtt                                                                     <  2:85772/248‑1 (MQ=255)                                                                                                                                    caccgTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTa                                                                    <  2:197781/249‑1 (MQ=255)                                                                                                                                                         gggATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATg                                                  <  2:196775/249‑1 (MQ=255)                                                                                                                                                          ggATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCg                                               <  2:165959/251‑1 (MQ=255)                                                                                                                                                          ggATTCTGGCGGGCGCGGCGATCGATATGCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTGGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCg                                               <  2:130313/251‑1 (MQ=255)                                                                                                                                                                      gcgcGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGat                                   <  2:31144/251‑1 (MQ=255)                                                                                                                                                                          ggcgATCGGTATGCCGGGCGGAATGCAGAGCGGCGGGTTTAAATGGTTGCTGCTGGCGACGGCGGTAGTTGTGT‑GGGTTGGGGGGTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTGTAAGTCATTATTTGGCGCGCGGCCG‑TTTGTTGTGGCTGACTCCGTTGATTTCTGCCATCGGCGTGGTGTCGCTGGTGGTTTTAATTCGCCGCTCGTTGATGCCGGTGTATATTGATAttt                               <  2:44097/251‑1 (MQ=255)                                                                                                                                                                            cgatcgatATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTg                             <  1:257885/251‑1 (MQ=255)                                                                                                                                                                                    atTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGt                     <  1:139366/251‑1 (MQ=255)                                                                                                                                                                                       ccagCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGAGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTTTCGCGCGGCCG‑TTTGTTGTGGCTGACGGCGTTGATGTCTGCCAGCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTTGTGCCGGTGTATATCGATATTTTGCGTAAAGTGGt                  <  1:241092/248‑1 (MQ=255)                                                                                                                                                                                                       gcggcgAGTTTAAATGGTGGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGAtt  <  1:71777/251‑2 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                 CAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCC‑AGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATA  >  NC_000913/71674‑72121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑