Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,875	T→C	G175G (GGT→GGC)	yabI →	DedA family protein YabI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,875	0	T	C	100.0%	76.8 / NA	28	G175G (GGT→GGC)	yabI	DedA family protein YabI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/18);  total (10/18)

AAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCC‑AGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATA  >  NC_000913/71629‑72121                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                         aaaCAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGt                                                                                                                                                                                                                                                      >  1:31144/1‑251 (MQ=255)                   tAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCC‑GACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTTCAGCCGGAATGTCGAGCGGCGAGGTTAAATGGTTTCTGCTggg                                                                                                                                                                                                                                   >  1:196220/1‑250 (MQ=255)                        cTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGGAGAGGGGCGAGTTTAAATGGTTGCTGCTGGcgacg                                                                                                                                                                                                                               >  1:198240/1‑247 (MQ=255)                                              caGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGAcggcgggatttgtggggggtggtg                                                                                                                                                                                                         >  1:79230/1‑233 (MQ=255)                                               aGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTAAGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGGTGCTGCTGGCGACGGCGGGATTTGGGT‑GGGTtggtgg                                                                                                                                                                                                       >  1:70463/1‑251 (MQ=255)                                                  aTGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGGTCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGGTTTTTTGT‑GGGTTTGtggctg                                                                                                                                                                                                    >  1:197781/1‑251 (MQ=255)                                                               tGGTCGGTCGCTTTGTTGTCCCGACGCGTCCGCTGGTGCCAATGGCGGCGGGCAGTATGGATCTGCCGGTGGCAAAATTCATTTCGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑CGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGGGTTTAAATGGTTGCTGCTGGCGACGGCGG‑GATTTGGTTGGGTTGGTgggtggcttttgtggcgg                                                                                                                                                                                       >  1:268400/1‑241 (MQ=255)                                                                 gtcggtcgCTTTGTTAGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTTCC‑TGGGATTCTGGCGGGAGCGGCGATCGATATTCCCGCCGGAATGCAGAGCGGCGAGGTTAAAAGGTTGCTGCTGGCGAAGGCGGTATTTTTGT‑GGGGTGGGGGCTGGCTTTGCTggggggt                                                                                                                                                                                     >  1:217854/1‑246 (MQ=255)                                                                             gttgGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCgg                                                                                                                                                                         <  1:47816/251‑1 (MQ=255)                                                                                        gggaccGCTGGGGCCCATTGTGGCGGTGATGCTGGAGATGGCGGTGGCGAAATTCATTACGCCGAATATTCTGGCCTGCCTGCGGTGGCCTCTGTTTTGCTCCCTGCCGAGGG‑TGCTGGCGGGCGCGGTGATCGGTATTCCAGCGGGAATGCAGAGCGGCGAGTGTAAATGGTTGCTGCTGGGGACGGCGGTGTGTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGcaaccg                                                                                                                                                              <  2:61791/247‑6 (MQ=255)                                                                                            ccGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGcaaccgatc                                                                                                                                                           <  1:229292/250‑9 (MQ=255)                                                                                                             ggCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTg                                                                                                                                          <  1:15759/250‑1 (MQ=255)                                                                                                                   aaTGCTGGATCTGCCGGTGGCAAAAGTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCGGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTcgcgcg                                                                                                                                   <  2:240090/251‑1 (MQ=255)                                                                                                                               gccggtgatacaaTTCATTACGCCGAATATTATCGGCTGCCGGCTGTGGCCACCGGTTTACTTCCTGCC‑GGGGATACTGGCGGGCGCGGCGATCGATATTCCAGGCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTGTGGCGCAGCGGTAAAGCACCCGATCGTTTAAGTCATTGTTTGTCGCGCGGCCG‑Attgtt                                                                                                                        <  2:268400/238‑1 (MQ=255)                                                                                                                                         aaaTTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTTTGGCGCAGCGGGAAAGCAACCCATCGTTTTAGTCATTATTTTTTGCGCGGGCG‑TTTTTTGTTGCttgcgcc                                                                                                            >  1:217954/1‑245 (MQ=255)                                                                                                                                                    gCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGGGCAGCGGGAAAGGAACCGATCGTTTTAGGCATTATTTTTCTCGCGGCCGGTTTGT‑GTGGGTGACGCCggtgttttttg                                                                                                 >  1:196775/1‑241 (MQ=255)                                                                                                                                                                     gcctgcTGTGGCCACCGTTTTTCTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGgc                                                                                 <  2:211933/250‑1 (MQ=255)                                                                                                                                                                            gtgGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtggt                                                                           <  2:144728/249‑1 (MQ=255)                                                                                                                                                                                ccaccgTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGtt                                                                     <  2:85772/248‑1 (MQ=255)                                                                                                                                                                                 caccgTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTa                                                                    <  2:197781/249‑1 (MQ=255)                                                                                                                                                                                                      gggATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATg                                                  <  2:196775/249‑1 (MQ=255)                                                                                                                                                                                                       ggATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCg                                               <  2:165959/251‑1 (MQ=255)                                                                                                                                                                                                       ggATTCTGGCGGGCGCGGCGATCGATATGCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTGGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCg                                               <  2:130313/251‑1 (MQ=255)                                                                                                                                                                                                                   gcgcGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGat                                   <  2:31144/251‑1 (MQ=255)                                                                                                                                                                                                                       ggcgATCGGTATGCCGGGCGGAATGCAGAGCGGCGGGTTTAAATGGTTGCTGCTGGCGACGGCGGTAGTTGTGT‑GGGTTGGGGGGTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTGTAAGTCATTATTTGGCGCGCGGCCG‑TTTGTTGTGGCTGACTCCGTTGATTTCTGCCATCGGCGTGGTGTCGCTGGTGGTTTTAATTCGCCGCTCGTTGATGCCGGTGTATATTGATAttt                               <  2:44097/251‑1 (MQ=255)                                                                                                                                                                                                                         cgatcgatATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTg                             <  1:257885/251‑1 (MQ=255)                                                                                                                                                                                                                                 atTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGt                     <  1:139366/251‑1 (MQ=255)                                                                                                                                                                                                                                    ccagCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGAGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTTTCGCGCGGCCG‑TTTGTTGTGGCTGACGGCGTTGATGTCTGCCAGCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTTGTGCCGGTGTATATCGATATTTTGCGTAAAGTGGt                  <  1:241092/248‑1 (MQ=255)                                                                                                                                                                                                                                                    gcggcgAGTTTAAATGGTGGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGAtt  <  1:71777/251‑2 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                         AAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCC‑AGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATA  >  NC_000913/71629‑72121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑