Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,857	T→A	P169P (CCT→CCA)	yabI →	DedA family protein YabI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,857	0	T	A	96.7%	87.4 / NA	31	P169P (CCT→CCA)	yabI	DedA family protein YabI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (11/18);  minor base G (0/1);  total (12/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCC‑AGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGT  >  NC_000913/71616‑72102                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                        cATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAAt                                                                                                                                                                                                                                                 <  1:41966/250‑1 (MQ=255)       tGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCagagg                                                                                                                                                                                                                                          >  1:85772/1‑250 (MQ=255)        aaagaaAAACAAATCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATTTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCAGGTGCCAATGG‑GGTGGGAAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATAGTATCGACTGCCTGCTGTTTCCACCGTTTTTCGTCCTGCC‑TGGGATTCTGGCGGGCGCTGCGGTCGATATTCCAGTGGGAATGCAGAGCg                                                                                                                                                                                                                                         <  2:245737/250‑1 (MQ=255)         aagaaAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTAAGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCgg                                                                                                                                                                                                                                        >  1:166446/1‑251 (MQ=255)              aaaCAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGt                                                                                                                                                                                                                                   >  1:31144/1‑251 (MQ=255)                                tAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCC‑GACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTTCAGCCGGAATGTCGAGCGGCGAGGTTAAATGGTTTCTGCTggg                                                                                                                                                                                                                >  1:196220/1‑250 (MQ=255)                                     cTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGGAGAGGGGCGAGTTTAAATGGTTGCTGCTGGcgacg                                                                                                                                                                                                            >  1:198240/1‑247 (MQ=255)                                                           caGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGAcggcgggatttgtggggggtggtg                                                                                                                                                                                      >  1:79230/1‑233 (MQ=255)                                                            aGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTAAGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGGTGCTGCTGGCGACGGCGGGATTTGGGT‑GGGTtggtgg                                                                                                                                                                                    >  1:70463/1‑251 (MQ=255)                                                               aTGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGGTCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGGTTTTTTGT‑GGGTTTGtggctg                                                                                                                                                                                 >  1:197781/1‑251 (MQ=255)                                                                            tGGTCGGTCGCTTTGTTGTCCCGACGCGTCCGCTGGTGCCAATGGCGGCGGGCA‑GTATGGATCTGCCGGTGGCAAAATTCATTTCGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑CGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGGGTTTAAATGGTTGCTGCTGGCGACGGCGG‑GATTTGGTTGGGTTGGTgggtggcttttgtggcgg                                                                                                                                                                    >  1:268400/1‑241 (MQ=255)                                                                              gtcggtcgCTTTGTTAGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTTCC‑TGGGATTCTGGCGGGAGCGGCGATCGATATTCCCGCCGGAATGCAGAGCGGCGAGGTTAAAAGGTTGCTGCTGGCGAAGGCGGTATTTTTGT‑GGGGTGGGGGCTGGCTTTGCTggggggt                                                                                                                                                                  >  1:217854/1‑246 (MQ=255)                                                                                          gttgGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCgg                                                                                                                                                      <  1:47816/251‑1 (MQ=255)                                                                                                     gggaccGCTGGGGCCCATTGTGGCGGTGA‑TGCTGGAGATGGCGGTGGCGAAATTCATTACGCCGAATATTCTGGCCTGCCTGCGGTGGCCTCTGTTTTGCTCCCTGCCGAGGG‑TGCTGGCGGGCGCGGTGATCGGTATTCCAGCGGGAATGCAGAGCGGCGAGTGTAAATGGTTGCTGCTGGGGACGGCGGTGTGTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGcaaccg                                                                                                                                           <  2:61791/247‑6 (MQ=255)                                                                                                         ccGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGcaaccgatc                                                                                                                                        <  1:229292/250‑9 (MQ=255)                                                                                                                          ggCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTg                                                                                                                       <  1:15759/250‑1 (MQ=255)                                                                                                                                aa‑TGCTGGATCTGCCGGTGGCAAAAGTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCGGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTcgcgcg                                                                                                                <  2:240090/251‑1 (MQ=255)                                                                                                                                             gccggtgatacaaTTCATTACGCCGAATATTATCGGCTGCCGGCTGTGGCCACCGGTTTACTTCCTGCC‑GGGGATACTGGCGGGCGCGGCGATCGATATTCCAGGCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTGTGGCGCAGCGGTAAAGCACCCGATCGTTTAAGTCATTGTTTGTCGCGCGGCCG‑Attgtt                                                                                                     <  2:268400/238‑1 (MQ=255)                                                                                                                                                       aaaTTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTTTGGCGCAGCGGGAAAGCAACCCATCGTTTTAGTCATTATTTTTTGCGCGGGCG‑TTTTTTGTTGCttgcgcc                                                                                         >  1:217954/1‑245 (MQ=255)                                                                                                                                                                  gCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGGGCAGCGGGAAAGGAACCGATCGTTTTAGGCATTATTTTTCTCGCGGCCGGTTTGT‑GTGGGTGACGCCggtgttttttg                                                                              >  1:196775/1‑241 (MQ=255)                                                                                                                                                                                   gcctgcTGTGGCCACCGTTTTTCTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGgc                                                              <  2:211933/250‑1 (MQ=255)                                                                                                                                                                                          gtgGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtggt                                                        <  2:144728/249‑1 (MQ=255)                                                                                                                                                                                              ccaccgTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGtt                                                  <  2:85772/248‑1 (MQ=255)                                                                                                                                                                                               caccgTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTa                                                 <  2:197781/249‑1 (MQ=255)                                                                                                                                                                                                                    gggATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATg                               <  2:196775/249‑1 (MQ=255)                                                                                                                                                                                                                     ggATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCg                            <  2:165959/251‑1 (MQ=255)                                                                                                                                                                                                                     ggATTCTGGCGGGCGCGGCGATCGATATGCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTGGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCg                            <  2:130313/251‑1 (MQ=255)                                                                                                                                                                                                                                 gcgcGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGat                <  2:31144/251‑1 (MQ=255)                                                                                                                                                                                                                                     ggcgATCGGTATGCCGGGCGGAATGCAGAGCGGCGGGTTTAAATGGTTGCTGCTGGCGACGGCGGTAGTTGTGT‑GGGTTGGGGGGTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTGTAAGTCATTATTTGGCGCGCGGCCG‑TTTGTTGTGGCTGACTCCGTTGATTTCTGCCATCGGCGTGGTGTCGCTGGTGGTTTTAATTCGCCGCTCGTTGATGCCGGTGTATATTGATAttt            <  2:44097/251‑1 (MQ=255)                                                                                                                                                                                                                                       cgatcgatATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTg          <  1:257885/251‑1 (MQ=255)                                                                                                                                                                                                                                               atTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGt  <  1:139366/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                        CATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCC‑AGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCG‑TTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGT  >  NC_000913/71616‑72102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑