Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,764	T→A	A138A (GCT→GCA)	yabI →	DedA family protein YabI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,764	0	T	A	95.7%	71.7 / ‑6.8	23	A138A (GCT→GCA)	yabI	DedA family protein YabI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (14/8);  minor base G (0/1);  total (14/9)
Fisher's exact test for biased strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCC‑AGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCG  >  NC_000913/71533‑71993                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                        ggcagggattgtcggttgtctgctGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAat                                                                                                                                                                                                                       <  1:114148/227‑1 (MQ=255)              ggttgtctgctGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGctgcct                                                                                                                                                                                                         >  1:235612/11‑251 (MQ=255)                            cGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGt                                                                                                                                                                                           >  1:66891/1‑251 (MQ=255)                                                           tttAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGgcgc                                                                                                                                                           >  1:144728/1‑251 (MQ=255)                                                              aaaaaGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCCGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCgcggg                                                                                                                                                        >  2:182661/1‑250 (MQ=255)                                                                        ttcATCGCTGAGCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGATCATGCGTTGCATCAACACATCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGatat                                                                                                                                               <  2:191078/248‑1 (MQ=255)                                                                                    cATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAAt                                                                                                                                   <  1:41966/250‑1 (MQ=255)                                                                                          tGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCagagg                                                                                                                            >  1:85772/1‑250 (MQ=255)                                                                                           aaagaaAAACAAATCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATTTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCAGGTGCCAATGG‑GGTGGGAAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATAGTATCGACTGCCTGCTGTTTCCACCGTTTTTCGTCCTGCC‑TGGGATTCTGGCGGGCGCTGCGGTCGATATTCCAGTGGGAATGCAGAGCg                                                                                                                           <  2:245737/250‑1 (MQ=255)                                                                                            aagaaAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTAAGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCgg                                                                                                                          >  1:166446/1‑251 (MQ=255)                                                                                                 aaaCAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGt                                                                                                                     >  1:31144/1‑251 (MQ=255)                                                                                                                   tAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCC‑GACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTTCAGCCGGAATGTCGAGCGGCGAGGTTAAATGGTTTCTGCTggg                                                                                                  >  1:196220/1‑250 (MQ=255)                                                                                                                        cTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGGAGAGGGGCGAGTTTAAATGGTTGCTGCTGGcgacg                                                                                              >  1:198240/1‑247 (MQ=255)                                                                                                                                              caGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGAcggcgggatttgtggggggtggtg                                                                        >  1:79230/1‑233 (MQ=255)                                                                                                                                               aGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTAAGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGGTGCTGCTGGCGACGGCGGGATTTGGGT‑GGGTtggtgg                                                                      >  1:70463/1‑251 (MQ=255)                                                                                                                                                  aTGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGGTCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGGTTTTTTGT‑GGGTTTGtggctg                                                                   >  1:197781/1‑251 (MQ=255)                                                                                                                                                               tGGTCGGTCGCTTTGTTGTCCCGACGCGTCCGCTGGTGCCAATGGCGGCGGGCA‑GTATGGATCTGCCGGTGGCAAAATTCATTTCGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑CGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGGGTTTAAATGGTTGCTGCTGGCGACGGCGG‑GATTTGGTTGGGTTGGTgggtggcttttgtggcgg                                                      >  1:268400/1‑241 (MQ=255)                                                                                                                                                                 gtcggtcgCTTTGTTAGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTTCC‑TGGGATTCTGGCGGGAGCGGCGATCGATATTCCCGCCGGAATGCAGAGCGGCGAGGTTAAAAGGTTGCTGCTGGCGAAGGCGGTATTTTTGT‑GGGGTGGGGGCTGGCTTTGCTggggggt                                                    >  1:217854/1‑246 (MQ=255)                                                                                                                                                                             gttgGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCgg                                        <  1:47816/251‑1 (MQ=255)                                                                                                                                                                                        gggaccGCTGGGGCCCATTGTGGCGGTGA‑TGCTGGAGATGGCGGTGGCGAAATTCATTACGCCGAATATTCTGGCCTGCCTGCGGTGGCCTCTGTTTTGCTCCCTGCCGAGGG‑TGCTGGCGGGCGCGGTGATCGGTATTCCAGCGGGAATGCAGAGCGGCGAGTGTAAATGGTTGCTGCTGGGGACGGCGGTGTGTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGcaaccg                             <  2:61791/247‑6 (MQ=255)                                                                                                                                                                                            ccGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGcaaccgatc                          <  1:229292/250‑9 (MQ=255)                                                                                                                                                                                                             ggCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTg         <  1:15759/250‑1 (MQ=255)                                                                                                                                                                                                                   aa‑TGCTGGATCTGCCGGTGGCAAAAGTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCGGCC‑TGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTcgcgcg  <  2:240090/251‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                        GGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCC‑AGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCG  >  NC_000913/71533‑71993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑