Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,701	T→C	R117R (CGT→CGC)	yabI →	DedA family protein YabI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,701	0	T	C	100.0%	75.4 / NA	23	R117R (CGT→CGC)	yabI	DedA family protein YabI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/7);  total (16/7)

GCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTT  >  NC_000913/71489‑71943                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     gcgcTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGc                                                                                                                                                                                                                <  1:23455/251‑1 (MQ=255)      gATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGTCGGGaa                                                                                                                                                                                                           <  1:188347/251‑1 (MQ=255)      gATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGaa                                                                                                                                                                                                           <  2:90662/251‑1 (MQ=255)                ggcgAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCggggaagttggggct                                                                                                                                                                                                 >  2:2690/1‑239 (MQ=255)                             tCTGGCACGCCTGGCTGGCAGGGATTGTCGGATGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGCCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTCGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGACAATGGAGGCGGGAA‑TGCTGGATCTGCCGGTGGcaaa                                                                                                                                                                                    >  1:158514/1‑247 (MQ=255)                                             ggcagggattgtcggttgtctgctGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAat                                                                                                                                                                    <  1:114148/227‑1 (MQ=255)                                                          ggttgtctgctGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGctgcct                                                                                                                                                      >  1:235612/11‑251 (MQ=255)                                                                        cGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGt                                                                                                                                        >  1:66891/1‑251 (MQ=255)                                                                                                       tttAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGgcgc                                                                                                         >  1:144728/1‑251 (MQ=255)                                                                                                          aaaaaGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCCGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCgcggg                                                                                                      >  2:182661/1‑250 (MQ=255)                                                                                                                    ttcATCGCTGAGCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGATCATGCGTTGCATCAACACATCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGatat                                                                                             <  2:191078/248‑1 (MQ=255)                                                                                                                                cATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAAt                                                                                 <  1:41966/250‑1 (MQ=255)                                                                                                                                      tGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCagagg                                                                          >  1:85772/1‑250 (MQ=255)                                                                                                                                       aaagaaAAACAAATCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATTTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCAGGTGCCAATGG‑GGTGGGAAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATAGTATCGACTGCCTGCTGTTTCCACCGTTTTTCGTCCTGCCTGGGATTCTGGCGGGCGCTGCGGTCGATATTCCAGTGGGAATGCAGAGCg                                                                         <  2:245737/250‑1 (MQ=255)                                                                                                                                        aagaaAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTAAGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCgg                                                                        >  1:166446/1‑251 (MQ=255)                                                                                                                                             aaaCAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGt                                                                   >  1:31144/1‑251 (MQ=255)                                                                                                                                                               tAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCC‑GACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTTCAGCCGGAATGTCGAGCGGCGAGGTTAAATGGTTTCTGCTggg                                                >  1:196220/1‑250 (MQ=255)                                                                                                                                                                    cTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGGAGAGGGGCGAGTTTAAATGGTTGCTGCTGGcgacg                                            >  1:198240/1‑247 (MQ=255)                                                                                                                                                                                          caGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGAcggcgggatttgtggggggtggtg                      >  1:79230/1‑233 (MQ=255)                                                                                                                                                                                           aGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTAAGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGGTGCTGCTGGCGACGGCGGGATTTGGGT‑GGGTtggtgg                    >  1:70463/1‑251 (MQ=255)                                                                                                                                                                                              aTGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGGTCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGGTTTTTTGT‑GGGTTTGtggctg                 >  1:197781/1‑251 (MQ=255)                                                                                                                                                                                                           tGGTCGGTCGCTTTGTTGTCCCGACGCGTCCGCTGGTGCCAATGGCGGCGGGCA‑GTATGGATCTGCCGGTGGCAAAATTCATTTCGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCCGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGGGTTTAAATGGTTGCTGCTGGCGACGGCGG‑GATTTGGTTGGGTTGGTgggtggcttttgtggcgg    >  1:268400/1‑241 (MQ=255)                                                                                                                                                                                                             gtcggtcgCTTTGTTAGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTTCCTGGGATTCTGGCGGGAGCGGCGATCGATATTCCCGCCGGAATGCAGAGCGGCGAGGTTAAAAGGTTGCTGCTGGCGAAGGCGGTATTTTTGT‑GGGGTGGGGGCTGGCTTTGCTggggggt  >  1:217854/1‑246 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     GCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGT‑GGGTTGGTGGCTGGCTGTGCTGGCGGTT  >  NC_000913/71489‑71943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑