Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,644	T→C	L98L (CTT→CTC)	yabI →	DedA family protein YabI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,644	0	T	C	100.0%	83.3 / NA	26	L98L (CTT→CTC)	yabI	DedA family protein YabI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/14);  total (12/14)

ATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGT  >  NC_000913/71402‑71879                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             aTGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAAc                                                                                                                                                                                                                                      <  1:75519/251‑1 (MQ=255)   ggCGGTGGTGGTGGTGGCCTTTCAGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGGGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGGTTCTGGCACGGCTGGCTGGCAGGGGTTGTCGGGTGGCTGCTGGGCGACAGGATCTCCTTCTGGGTGGGCTGGGGTTTTTAAAAACCGTTGCATTGCTGGGCATTTTTGAAGAAAAAAAAAAGAATACTCGATaaaaatg                                                                                                                                                                                                                                    >  1:211933/1‑248 (MQ=255)       gtggtgTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGGCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACa                                                                                                                                                                                                                                <  1:164887/251‑1 (MQ=255)              tggtggCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGtt                                                                                                                                                                                                                          <  2:89535/250‑1 (MQ=255)               ggtggCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGAATCTACCAGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGCTTGGCAGCGGCGAGTTAAGTTTCTGGAACGCCTGGCTGGCAGGGGATGTCGGGTGTCTGCTGGGCGACTGGGTCTCCTTCTGGCTGGGCTGGCGGTTTAAAAAGCCGCTGCATCGACGGTCATTTCTGGAGAAAAAACAAGCCTTACTCGGTAAAACAGAACATGAGTTGc                                                                                                                                                                                                                        >  1:44097/1‑251 (MQ=255)                          tGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACGCGATAAAACTGAACATGCGTTGCATCAACACAg                                                                                                                                                                                                              <  2:106320/250‑1 (MQ=255)                                      cgctggTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCAtt                                                                                                                                                                                                 <  1:184893/251‑1 (MQ=255)                                              ggTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGGTGGGGGCGCTGATTGGCAGCGGCGAGTTTAGTTTCTGGGACGCCTGGCTGGCCGGGGTTGTCGGTTGGCTGCTGGGCGGCCGGATCTCCTTCTGGCTGGGCTGGCGTTTTTAAAAGACGTTGCATCGCTGGGCATTTTTGTAGAAAAAACAAGCATTACTCGCTAAAACTGAACCTGCGTTGCATCAACACAGCCTGTTCACCATTCTtgtcgg                                                                                                                                                                                         >  2:111874/1‑251 (MQ=255)                                                  tGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGgtcggtcg                                                                                                                                                                                      <  1:132457/250‑1 (MQ=255)                                                    gttCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGTATTGTCGGTTGTCTGCTGGTCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAGACTGAACATGCGTTGCATCAACACAGCATGTTCTCCATTCTGgtcggtcgctt                                                                                                                                                                                   <  2:80310/250‑4 (MQ=255)                                                                                        gcgcTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGc                                                                                                                                               <  1:23455/251‑1 (MQ=255)                                                                                             gATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGTCGGGaa                                                                                                                                          <  1:188347/251‑1 (MQ=255)                                                                                             gATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGaa                                                                                                                                          <  2:90662/251‑1 (MQ=255)                                                                                                       ggcgAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCggggaagttggggct                                                                                                                                >  2:2690/1‑239 (MQ=255)                                                                                                                    tCTGGCACGCCTGGCTGGCAGGGATTGTCGGATGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGCCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTCGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGACAATGGAGGCGGGAA‑TGCTGGATCTGCCGGTGGcaaa                                                                                                                   >  1:158514/1‑247 (MQ=255)                                                                                                                                    ggcagggattgtcggttgtctgctGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAat                                                                                                   <  1:114148/227‑1 (MQ=255)                                                                                                                                                 ggttgtctgctGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGctgcct                                                                                     >  1:235612/11‑251 (MQ=255)                                                                                                                                                               cGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGt                                                                       >  1:66891/1‑251 (MQ=255)                                                                                                                                                                                              tttAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGgcgc                                        >  1:144728/1‑251 (MQ=255)                                                                                                                                                                                                 aaaaaGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCCGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCgcggg                                     >  2:182661/1‑250 (MQ=255)                                                                                                                                                                                                           ttcATCGCTGAGCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGATCATGCGTTGCATCAACACATCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGatat                            <  2:191078/248‑1 (MQ=255)                                                                                                                                                                                                                       cATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAAt                <  1:41966/250‑1 (MQ=255)                                                                                                                                                                                                                             tGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCagagg         >  1:85772/1‑250 (MQ=255)                                                                                                                                                                                                                              aaagaaAAACAAATCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATTTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCAGGTGCCAATGG‑GGTGGGAAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATAGTATCGACTGCCTGCTGTTTCCACCGTTTTTCGTCCTGCCTGGGATTCTGGCGGGCGCTGCGGTCGATATTCCAGTGGGAATGCAGAGCg        <  2:245737/250‑1 (MQ=255)                                                                                                                                                                                                                               aagaaAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTAAGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCgg       >  1:166446/1‑251 (MQ=255)                                                                                                                                                                                                                                    aaaCAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGt  >  1:31144/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             ATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGT  >  NC_000913/71402‑71879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑