Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,639	C→T	L97L (CTA→TTA)	yabI →	DedA family protein YabI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,639	0	C	T	96.2%	79.7 / NA	26	L97L (CTA→TTA)	yabI	DedA family protein YabI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (11/14);  minor base A (1/0);  total (12/14)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGT  >  NC_000913/71394‑71879                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  aTTCATTGGTGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCattact                                                                                                                                                                                                                                               <  2:262906/250‑1 (MQ=255)         aTGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAAc                                                                                                                                                                                                                                      <  1:75519/251‑1 (MQ=255)           ggCGGTGGTGGTGGTGGCCTTTCAGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGGGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGGTTCTGGCACGGCTGGCTGGCAGGGGTTGTCGGGTGGCTGCTGGGCGACAGGATCTCCTTCTGGGTGGGCTGGGGTTTTTAAAAACCGTTGCATTGCTGGGCATTTTTGAAGAAAAAAAAAAGAATACTCGATaaaaatg                                                                                                                                                                                                                                    >  1:211933/1‑248 (MQ=255)               gtggtgTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGGCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACa                                                                                                                                                                                                                                <  1:164887/251‑1 (MQ=255)                      tggtggCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGtt                                                                                                                                                                                                                          <  2:89535/250‑1 (MQ=255)                       ggtggCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGAATCTACCAGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGCTTGGCAGCGGCGAGTTAAGTTTCTGGAACGCCTGGCTGGCAGGGGATGTCGGGTGTCTGCTGGGCGACTGGGTCTCCTTCTGGCTGGGCTGGCGGTTTAAAAAGCCGCTGCATCGACGGTCATTTCTGGAGAAAAAACAAGCCTTACTCGGTAAAACAGAACATGAGTTGc                                                                                                                                                                                                                        >  1:44097/1‑251 (MQ=255)                                  tGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACGCGATAAAACTGAACATGCGTTGCATCAACACAg                                                                                                                                                                                                              <  2:106320/250‑1 (MQ=255)                                              cgctggTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCAtt                                                                                                                                                                                                 <  1:184893/251‑1 (MQ=255)                                                      ggTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGGTGGGGGCGCTGATTGGCAGCGGCGAGTTTAGTTTCTGGGACGCCTGGCTGGCCGGGGTTGTCGGTTGGCTGCTGGGCGGCCGGATCTCCTTCTGGCTGGGCTGGCGTTTTTAAAAGACGTTGCATCGCTGGGCATTTTTGTAGAAAAAACAAGCATTACTCGCTAAAACTGAACCTGCGTTGCATCAACACAGCCTGTTCACCATTCTtgtcgg                                                                                                                                                                                         >  2:111874/1‑251 (MQ=255)                                                          tGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGgtcggtcg                                                                                                                                                                                      <  1:132457/250‑1 (MQ=255)                                                            gttCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGTATTGTCGGTTGTCTGCTGGTCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAGACTGAACATGCGTTGCATCAACACAGCATGTTCTCCATTCTGgtcggtcgctt                                                                                                                                                                                   <  2:80310/250‑4 (MQ=255)                                                                                                gcgcTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGc                                                                                                                                               <  1:23455/251‑1 (MQ=255)                                                                                                     gATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGTCGGGaa                                                                                                                                          <  1:188347/251‑1 (MQ=255)                                                                                                     gATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGaa                                                                                                                                          <  2:90662/251‑1 (MQ=255)                                                                                                               ggcgAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCggggaagttggggct                                                                                                                                >  2:2690/1‑239 (MQ=255)                                                                                                                            tCTGGCACGCCTGGCTGGCAGGGATTGTCGGATGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGCCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTCGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGACAATGGAGGCGGGAA‑TGCTGGATCTGCCGGTGGcaaa                                                                                                                   >  1:158514/1‑247 (MQ=255)                                                                                                                                            ggcagggattgtcggttgtctgctGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAat                                                                                                   <  1:114148/227‑1 (MQ=255)                                                                                                                                                         ggttgtctgctGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGctgcct                                                                                     >  1:235612/11‑251 (MQ=255)                                                                                                                                                                       cGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGt                                                                       >  1:66891/1‑251 (MQ=255)                                                                                                                                                                                                      tttAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGgcgc                                        >  1:144728/1‑251 (MQ=255)                                                                                                                                                                                                         aaaaaGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCCGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCgcggg                                     >  2:182661/1‑250 (MQ=255)                                                                                                                                                                                                                   ttcATCGCTGAGCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGATCATGCGTTGCATCAACACATCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGatat                            <  2:191078/248‑1 (MQ=255)                                                                                                                                                                                                                               cATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAAt                <  1:41966/250‑1 (MQ=255)                                                                                                                                                                                                                                     tGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCagagg         >  1:85772/1‑250 (MQ=255)                                                                                                                                                                                                                                      aaagaaAAACAAATCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATTTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCAGGTGCCAATGG‑GGTGGGAAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATAGTATCGACTGCCTGCTGTTTCCACCGTTTTTCGTCCTGCCTGGGATTCTGGCGGGCGCTGCGGTCGATATTCCAGTGGGAATGCAGAGCg        <  2:245737/250‑1 (MQ=255)                                                                                                                                                                                                                                       aagaaAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTAAGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCgg       >  1:166446/1‑251 (MQ=255)                                                                                                                                                                                                                                            aaaCAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGt  >  1:31144/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  ATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAA‑TGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGT  >  NC_000913/71394‑71879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑